Saratov JOURNAL of Medical and Scientific Research

2014, volume 10

Psychological characteristics of patients with functional and inflammatory bowel disorders

Year: 2014, volume 10 Issue: №1 Pages: 80-85
Heading: Internal Diseases Article type: Original article
Authors: Kozlova I.V., Myalina Yu.N., Lekareva L.I., Badieva O.E., Tikhonova Т.А.
Organization: Saratov State Medical University

Objective: to study the psychological characteristics of patients with irritable bowel syndrome (IBS), ulcerative colitis (UC), Crohn's disease (CD). Material and methods. The study group included 98 patients with IBD (inflammatory bowel disease) and IBS, the control group included 30 healthy individuals. Set of psychological tests included questionnaire (multifactorial systemic examination of the person), the Luscher color test, Beck Depression Inventory, a test on health, activity, mood. Results. Premorbid personality traits, communication disorders with stress have been revieled. According to the nosology different types of emotional response to the disease, changes in health and activity have been marked. There is a high level of frustration needs, increased frequency of anxiety and depression in all patients. Conclusion. Psychological mechanisms of pathology are similar in functional and organic bowel diseases with the greatest influence on the course of functional disorders.

Keywords: inflammatory bowel disease, irritable bowel syndrome (IBS)

Ultrasound Diagnostics of Premature Birth

Year: 2014, volume 10 Issue: №1 Pages: 74-79
Heading: Obstetrics and Gynecology Article type: Review
Authors: Chekhonatskaya M.L., Vasilevich L.K., Petrosyan N.O., Kolesnikova E.A.
Organization: Clinical hospital n.a. S. R. Mirotvortsev SSMU, Saratov Clinical Hospital № 8, Saratov State Medical University

The purpose of the article is to analyze the data of native and foreign literature and to consider the modern aspects of ultrasound diagnostics of premature birth, and new additional criteria for early diagnostics and prediction of preterm delivery. Hemodynamic characteristics in the second and third trimesters of the pregnancy have been covered.

Keywords: Doppler study, prematurely-born, ultrasound diagnostics

The contemporary methods of patients with intraperitoneal bleeding of genital origin treatment objective efficacy assessment

Year: 2014, volume 10 Issue: №1 Pages: 70-74
Heading: Obstetrics and Gynecology Article type: Original article
Authors: Nazarenko O.Ya., Timofeyeva S.V.
Organization: Military-Medical Clinical Centre of the South Region, (Odessa, Ukraine), Ukrainian Scientific Research Institute of transport medicine, Odessa (Ukraine)

The aim of the article: the peculiarities of the modern methods of treatment efficiency objective assessment in considerable contingent of patients with intraperitoneal bleeding of a genital origin are investigated. Material et metods: The original data of intra- and postoperative medical interventions efficiency are given and analyzed in 78 patients who were operated laparoscopically owing to intraperitoneal bleedings up to 500 ml of blood. Results. Circulating blood volume in these patients was restituted by blood Autohaemoreinfusion together with donors' blood and blood-substituting compounds injection. Red blood cells and hemoglobin content together with heart rate variability indexes were taken as the markers of the patients' organism functional condition and rehabilitation efficacy estimation. One month after miniinvazive treatment in women with acute intraperitoneal blood loss above 500 ml one could see certain sympathetic activation with reciprocal parasympathetic activity suppression together with pertinent activation degree value normalization. Conclusions. The conclusion was made that HRV data analysis allows to make an objective estimation of women with acute genital intraperitoneal bleeding (above 500 ml) treatment efficacy. The last is very important in the prognostic aspect because there are few objective methods of afteroperational state analysis in patients with bleeding of different origin.

Keywords: cardio-intervalometry, compensatory-adaptative mechanisms, extrauteral pregnancy, haemoperitoneum, heart rate variability, ovarian apoplexy

Ultrasound diagnostics of postpartum endometritis

Year: 2014, volume 10 Issue: №1 Pages: 65-69
Heading: Obstetrics and Gynecology Article type: Review
Authors: Bratchikova О.А., Chekhonatskaya M.L., Yannaeva N.E.
Organization: Saratov State Medical University

The purpose of the article is to present data on diagnostics of postpartum infectious diseases in women in childbirth. The aim of the conducted survey is to study modern ultrasound diagnostic methods of the given pathology.

Keywords: ultrasound diagnostics, postpartum infections, endometritis

Procalcitonin as a diagnostic test for thermal injury

Year: 2014, volume 10 Issue: №1 Pages: 61-64
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Bozhedomov A.J.
Organization: Saratov State Medical University

Purpose: to determine the diagnostic value of procalcitonin in blood serum with probable development of multiple-organ-failure syndrome in thermal injury. Material and Methods. Procalcitonin test has been performed on the 1st, 3d, 7th, 15th and 30th days after the thermal injury. The expression of systemic inflammatory response syndrome (SIRS), leukocytic intoxication index (Lll) calculated according to Ya.Ya. Kalf-Kalif, levels of C-reactive protein, MCP-1, IL-6, VEGF, endothelin, and a number of circulating endothelial cells have been determined. Results. The increased level of procalcitonin (>0,5 ng/ml) in thermal injury has been observed in 42,7% of recovered patients and in 71,3% of dead patients. Conclusion. It has been found out that procalcitonin test in thermal injury may be used as an early marker for patients liable to develop polyorgan insufficiency syndrome.

Keywords: burns, procalcitonin, SIRS

Gender features of rheological properties of blood (plasma viscosity, aggregation and deformation of erythrocytes) in patients with chronic generalized periodontitis

Year: 2014, volume 10 Issue: №1 Pages: 56-61
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Kazantsev A.V., Suyetenkov D.Ye.,/Andronoy E.V./, J Firsova I.V.
Organization: Saratov State Medical University

The goal is to study gender features of rheological properties of blood in patients with chronic generalized periodontitis (CGP) from mild to moderate severity. Material and Methods. 80 patients (43.8% male) with CGP aged 42±5 years have been studied. 41 patients (43.9% male) experienced mild severity of CGP, and 39 patients (43.6% male) experienced moderate severity. 40 healthy adults (50% male), aged 31±7 years, have been included into the study. Plasma viscosity on shear rate values 300 sec1 to 5 sec1, and rheological features of erythrocytes (aggregation and deformation) have been evaluated. Results. In healthy women and women with mild severity of CGP, plasma viscosity was lower than in the similar group of men. The rheological features of erythrocytes have not gender differences among healthy adults and patients with mild CGP. Increased severity of CGP has been associated with increased plasma viscosity and rheological features of erythrocytes, particularly in men. Conclusion. Plasma viscosity differs in men and women (childbearing age) (healthy subjects and patients with CGP). In women, severity of pathological changes of plasma viscosity and rheological features of erythrocytes have been determined to be lower than in men.

Keywords: aggregation of erythrocytes, blood viscosity, chronic generalized periodontitis, deformation of erythrocytes

Variability of cephalometric parameters of men with orthognathic and direct occlusion

Year: 2014, volume 10 Issue: №1 Pages: 52-55
Heading: Macro- and Micromorphology Article type: Original article
Authors: Sheludko S.N., Muzurova L.V., Konnov V.V., Mikheeva M.V.
Organization: Saratov State Medical University

The aim: to study the variability of morphometric parameters of the head of men of 21-35 years old, with orthognathic and direct occlusion and identify their differences. Material and Methods. Cephalometry performed in 93 men aged 21 to 35 years from the Saratov region. Distribution groups conducted for the type of physiological occlusion: orthognathic (n=61) and direct (n=32). To determine the parameters used cephalometric anthropometric standard set of tools. On the head cephalometric points were determined between which measured morphometric parameters of the cranial and facial departments, as well as parameters of soft tissue structures. Results. In men with physiological occlusion parameters having statistically significant differences in orthognathic and direct bite were identified: head length, morphological face height, the height of nasal and maxillary division, mandibular body height, width of the face, the angular width of the face. Average values of these parameters are more prevalent in men who have a direct bite.
Conclusion. The data obtained should be considered during orthodontic and orthopaedic treatment, with lectures and practical training in the departments and faculties of the human anatomy with the dental profile.

Keywords: cephalometriy, orthognathic and direct occlusion

Pathomorphological cardial changes in the sudden infant death syndrome

Year: 2014, volume 10 Issue: №1 Pages: 48-52
Heading: Macro- and Micromorphology Article type: Original article
Authors: Rozumny D.V.
Organization: Samara State Medical University

In order to establish the relationship between the timing of fetal obliteration of communication and expression remodeling of the heart chambers, as one of the possible causative factors of SIDS, the selected material has been based on macromorphometric study. Material included 43 hearts from infants who died of SIDS. The control group consisted of 48 hearts of children suddenly dead from aspiration of gastric contents and infectious disease. The methods composed a macroscopic examination of the heart by the method of separate weighing of the complex morphometric studies, histological examination (used the following stains: hematoxylin and eosin, picrofuchsin by Van Gieson, Nissl's stain), immunohistochemical method using markers of neurofilament. The results revealed that in children who died of SIDS, obliteration of the fetal communication occurs essentially in the late period —to the age of 8 months, and the mass of the left and right ventricles of the heart exceeds the age limit of normal due to the prevalence of ventricular muscle mass and hypertrophy of cardiomyocytes. In all parts of the heart subendocardial fibrosis in the ventricles — pronounced interstitial fibrosis has been observed. In the interatrial septum at the location at the oval window morphometric study resulted in significant predominance area percentage of connective tissue in relation to the muscle tissue. On histological sections elements of the nervous tissue — randomly arranged nerve ganglia with degenerative changes and nerve fibers were identified. Conclusion. Finally, it should be noted that the growth of cardiosclerosis in all parts of the heart in newborns and infants, who died suddenly from SIDS, extends the terms of obliteration of fetal communications, and degenerative changes in the nerve ganglia that lead to the disturbance of supply and distribution of nerve impulses that pass through them. These factors contribute to the development of hemodynamic disturbances and may be important in the development of sudden death in children.

Keywords: sudden infant death syndrome, heart, fetal communications

The study of changes of biophysical properties of red blood cells in storage in erythrocyte-containing solutions using atomic force microscopy

Year: 2014, volume 10 Issue: №1 Pages: 44-48
Heading: Macro- and Micromorphology Article type: Original article
Authors: Lamzin I.M., Khayrullin R.М.
Organization: Ulyanovsk State University

Currently the departments of quality control of blood centers evaluate the suitability for clinical use of erythrocyte-containing solutions indirectly by routine methods of the determination of hemoglobin concentration, hematocrit level, or hemolysis at the end of the period of storing. These methods can not directly characterize the state of membranes of preserved erythrocytes. The aim of the work is to study the changes of elasticity and surface potential of membranes of red blood cells of erythrocyte-containing solutions in storage for 35 days. Material and Methods. Two series of dry cytological preparations (smears) of erythrocytes have been investigated. The first group consisted of 8 samples of erythrocyte-containing solutions prepared on the day of preservation, and the second group included 20 samples prepared after the long term storage for 35 days at t=+4°C. Blood was stored in bags of «Baxter» company (USA) conserving with «CPDA-1». For atomic force microscopy five typical erythrocytes were selected and their elasticity was measured at 9 points of the membrane of each erythrocyte. Total number of measurements was 1296. Results. Average value of Young's modulus of the first group of samples was 1,81±0,02 (M±m) KPa. The second group showed 3,22±0,02 KPa statistically higher (p

Keywords: atomic force microscopy, erythrocyte-containing solutions, erythrocytes, Young's modulus

Mathematical modeling of structural indices of thyroid gland in thyroid pathology

Year: 2014, volume 10 Issue: №1 Pages: 38-44
Heading: Macro- and Micromorphology Article type: Original article
Authors: Kalmin O.V., Kalmin О.О..
Organization: Penza State University

Purpose: the complete analysis of the morphological parameters of the thyroid gland in various forms of thyroid pathology by methods of mathematical modeling. Material and Methods. Histologically examined samples of operating thyroid glands of 199 patients of both sexes aged 21 to 74 years, were treated surgically. Experimental data were studied by factorial and discriminant analysis. Results. When the factor analysis of 60 experimental parameters were identified 14 major factors that indicate 14 main trends in the dynamics of the structure of the thyroid gland due to an increase in severity of pathological processes. Discriminant analysis showed that the study of quantitative structure of the thyroid gland there may be cases of erroneous inclusion in the theoretical option groups, however, the study of the entire set of indicators observed a 100% correct distribution of cases by groups of pathology. Conclusion. As a result, the factor of the original system of 60 morphological parameters of thyroid pathology in various forms has been minimized to 14 new uncorrelated parameters (main factors), reflecting changes in the morphology of the basic laws of the pathology of the gland. As a result of the discriminant analysis of morphological parameters of the thyroid gland in different types of its pathology the importance of semi-quantitative parameters of thyroid structure has been revealed.

Keywords: discriminant analysis, factor analysis, morphometry, thyroid gland