Saratov JOURNAL of Medical and Scientific Research

2010, volume 6

Kinetiks of cellular populations under influence of infra-red low-power laser irradiation

Year: 2010, volume 6 Issue: №1 Pages: 023-026
Heading: Macro- and Micromorphology Article type: Original article
Authors: I.O. Bugaeva, A.V. Egorova, O.V. Zlobina
Organization: Saratov State Medical University
Summary:

The morphokinetiks of cellular populations under the influence of infra-red low-power laser irradiation has been studied. our researchers evidenced, that the number of cortical thymocytes decreases under the influence of infra-red low-power laser irradiation. Invasion of Mast cells in to the stroma of thymus and their contact with the reticuloepithelio-cytes are the important factors for the stimulation of proliferation and differentiation of thymocytes

Keywords: thymus, infra-red low-power laser irradiation, cellular populations

Risk assessment of ecotoxicant effect on children's health

Year: 2010, volume 6 Issue: №1 Pages: 018-022
Heading: Hygiene Article type: Original article
Authors: M.N. Ivanchenko, I.N. Lutsevich, V.V. Zhukov
Organization: Saratov State Medical University
Summary:

Assessment of health risk level is the major methodological approach for hygienic monitoring planning, for cause-effect basis of interrelation between environmental pollution and health. Pollution with heavy metals (cadmium, nickel) of Zavodskoy district in Saratov results in increasing risk and this requires development of health protection ways and approaches

Keywords: heavy metals, health risk

Molecular-genetic assessment of angiotensin-converting enzyme hyperactivity determinancy risk at hemorrhagic fever with renal syndrome

Year: 2010, volume 6 Issue: №1 Pages: 014-017
Heading: Biochemistry Article type: Original article
Authors: A.A. Baygildina, D.V. Islamgulov, F.Kh. Kamilov, T.A. Khabelova, I.R. Minniakhmetov
Organization: Ufa scientific centre of the RAS, Bashkir State Medical University of the Russian Health Care
Summary:

The research aimed to explore the changes of angiotensin-converting enzyme (ACE) blood activity and polymorphism of the angiotensin II type 1 receptor gene as disease predictor at hemorrhagic fever with renal syndrome (HFRS). In exami¬nation 409 patients at the age of 15-65 years were involved. ACE blood activity with the help of Bbhlmann (Switzerland) ACE kinetic test was determined. Blood genomic DNA by phenol- chloroformic extraction was isolated, genetic locuses by polymerase chain reaction of DNA synthesis were researched. It was shown that the ACE blood activity at HFRS is risen and these changes the significanter the severer form of disease. Nonsignificant rising of enzyme activity only in feveric period of both ungravic and gravic forms was observed. In gravic form significant changes of ACE activity are observed, in complicated form - stable high enzyme activity during all disease took place. Analysis of angiotensin II type 1 receptor gene polymorphism shown that alleles *A1166 and *C1166, genotypes *A1166/*A1166 and *C1166/*C1166 are not associated with HFRS severity. Made conclusion that high ACE activity is not adaptive reaction due to defect in angiotensin II binding and it is an adequate metabolic response of an organism to endotheliotropic virus action

Keywords: gene of angiotensin II type 1 receptor, angiotensin-converting enzyme

Comparative study of antibacterial action of iron and copper nanoparticles on clinical Staphylococcus aureus strains

Year: 2010, volume 6 Issue: №1 Pages: 011-014
Heading: Biochemistry Article type: Original article
Authors: I.V. Babushkina, V.B. Borodulin, G.V. Korshunov, D.M. Puchinjan
Organization: Saratov State Medical University, Saratov Scientific Research Institute of Traumatology and Orthopedics
Summary:

Research objective is to study antibacterial action of nanoparticles of iron and copper on polyantibiotically resistant clinical Staphylococcus aureus strains.
Materials and methods include antibacterial action of nanoparticles of copper and iron on 10 Staphylococcus au¬reus strains, isolated from patients with purulent complications stayed in the in-patient department of traumatology and orthopedics. Solutions of powders of iron and copper have been prepared directly before the experiment in concentra¬tion from 0,001 to 1 mg/ml.
it has been revealed that the influence of nanoparticles on growth of clinical strains and the intensity of antibacterial effect depends on the form of nanoparticles, their concentration and action time. concentration of 0,1 mg/ml and 1 mg/ ml of iron nanoparticles has provoked the decrease in quantity of microbe cells from 3 to 34 % (p <0,01). in smaller concentrations the reliable antibacterial effect has not been observed. Antibacterial activity of copper nanoparticles has been expressed in a wide range of concentrations from 0,001 mg/ml to 1 mg/ml, even during short-term action (30 minutes) it has provoked reduction of quantity of the microbe cells grown on the firm nutrient medium, 97-100 % in comparison with the control (p <0,001).
in conclusion it is to point out that copper nanoparticles have more expressed inhibitory effect on growth of clini¬cal strains of golden staphylococcus than iron nanoparticle suspension. inhibition degree depends on superdispersed powder dosage and incubation period

Keywords: Staphylococcus aureus, nanoparticles, iron, copper