Saratov JOURNAL of Medical and Scientific Research

Experimental study of synthetic polymeric materials as a basis in the creation of the advaced carrier matrix for the cultivation of limbal stem cells

Year: 2019, volume 15 Issue: №2 Pages: 495-501
Heading: Ophtalmology Article type: Original article
Authors: Karpovich V.V., Kulikov A.N., Churashov S.V., Chernysh V.F., Grigoriev S.G., Blinova M.I., Nashchekina Yu.A., Aleksandrova O.I., Khorolskaya Yu.I., Nikonov P.O., Tsobkallo E.S., Moskalyuk O.A., Melnikov A.S., Serdobintsev P.Yu., Mashel' T.V., Pisugina G.A., Perepletchikova D.A., Khoroshikh D.A.
Organization: Institute of Cytology, Russian Academy of Sciences, St. Petersburg Polytechnic University, St. Petersburg State University, St. Petersburg State University of Industrial Technology and Design

Purpose: to study in the experiment the properties of three diferent types of synthetic polyester matrices, to conduct their comparative assessment and determine the optimum as a carrier for the cultivation and transplantation of limbal stem cells while eliminating limbal insufciency. Material and Methods. Transparency, mechanical properties (strength, elongation at break, modulus of rigidity), biocompatibility with cell cultures of the cornea, as well as the study of the timing of matrix biodegradation in vivo were carried out. Results. The study examined the optical and mechanical properties of matrices made from polylactide-glycolide (PLG), polylactide-caprolactone (PLC) and poly-e-caprolactone (PCL). It was experimentally shown that human and rabbit limbal stem cells, as well as human corneal epithelium cells, adhered on the surface of all types of matrices under investigation, and during cultivation they retained the typical structure of actin cytoskeleton, the ability to proliferate and migrate. Diferences in the interaction of diferent cell cultures with diferent types of carriers were revealed. The terms of biodegradation of PLC matrices with a thickness of 5 μm was about 30 days. Conclusion. The results indicate that it is possible to use 5 μm thick PLC matrices as a carrier of cultured limbal stem cells.

1 Global initiative for the elimination of avoidable blindness. Geneva: World Health Organization, 1997; p. 48
2 Pascolini D. Global estimates of visual impairment: 2010 Br J Ophthalmol 2012; 96 (5): 614–8
3 Lim AS. Mass blindness has shifted from infection (onchocerciasis, trachoma, corneal ulcers) to cataract. Ophthalmologica 1997; 211 (4): 270 p.
4 Libman ES, Shakhova EV. Blindness and visually impaired in the population of Russia. In: VIII Congress of Russian ophthalmologists. Moscow, 2005; p. 78–9
5 Kasparov AA. Treatment of the most important diseases of the cornea. Proceedings of the In: Eighth Congress of Ophthalmologists of Russia. Moscow, 2005; p. 450–1
6 Maychuk YuF. Major trends in epidemiology and treatment of eye infections. In: Abstracts of the VIII congress of ophthalmologists of Russia. Moscow, 2005; p. 92–3
7 Slonimsky AYu. Opportunities for end-to-end cornea transplantation in various pathologies of the anterior segment of the eye. Clinical Ophthalmology 2001; (3): 102–5
8 Reinhard Т. Corneal Disease: Recent Developments in Diagnosis and Therapy. Springer-Verlag-Berlin, Heidelberg, 2013; 121 p.
9 Chernysh VF, Boyko EV. Eye burns: State of the problem and new approaches. Moscow: GEOTAR-Media, 2017; 184 p.
10 Holland EJ, Schwartz GS. The evolution of epithelial transplantation for severe ocular surface disease and a proposed classifcation system. Cornea 1996; 15: 549–56
11 Grueterich M, Espana EM, Tseng SC. Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche. Surv Ophthalmol 2003; 4 (6): 631–46
12 Pellegrini G, Traverso CE, Franzi AT, et al. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997; 349 (9057): 990–3
13 Sefat F, et al. Production, Sterilization and Storage of Biodegradable Electrospun PLGA Membranes for Delivery of Limbal Stem Cells of the Cornea. Procedia Engineering 2013; 59: 101–16
14 Kulikov AN, Churashov SV, Chernysh VF, et al. Current approaches to the problem of selection carrier for cultivation limbal stem cells in the treatment of limbal stem cell defciency. Oftalmologiceskie vedomosti 2018; 11 (2): 48–56
15 Neroev VV, Gundorova RA, Makarov PV, et al. Eye burns: a guide for doctors. Moscow: GOTAR-Media, 2013; 224 p.

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