Saratov JOURNAL of Medical and Scientific Research

Grigoriev S.G.

Military Medical Academy n. a. S. M. Kirov, Senior Researcher of the Scientifc and Research centre, Professor, DSc

Dynamics of the neovascular process activity in proliferative diabetic reti-nopathy after panretinal laser coagulation using lasers with different wavelengths

Year: 2019, volume 15 Issue: №4 Pages: 843-847
Heading: Ophtalmology Article type: Original article
Authors: Gatsu M.V., Borzilova Yu.A., Grigoriev S.G.
Organization: North-West State Medical University n.a. I.I. Mechnikov, Department of Neuropathology Assistant Professor, Candidate of Medical Science

Purpose: comparative evaluation of the effectiveness of panretinal laser coagulation performed using laser radiation with wavelengths of 532 and 577 nm in the treatment of proliferative diabetic retinopathy. Material and Methods. The study included 32 patients (64 eyes) with stage 1-3 proliferative retinopathy. Patients were divided into two groups by random sampling depending on the wavelength of laser radiation used for PRC (577 nm — group 1, and 532 nm — group 2). The neovascular process activity was assessed by the dynamics of the fluorescence-angiographic picture and the level of vasoproliferative factor in the tear 3, 6 and 12 months after surgery. Results. The conducted studies have shown that laser coagulation performed by both wavelengths is an effective treatment for retinopathy, but gives a delayed effect. After 1-3 months in the first group and 1-6 months in the second one after its implementation, there is an increase in the neovascularization activity and an increase in the VEGF-A level in the lacrimal fluid. In terms of the number and severity of temporary side effects, PRC is the leader using a wavelength of 532 nm, which is eliminated only by 12 months of observation. Conclusion. When using a 577 nm laser, the neovascular process is most quickly stabilized (p<0.05), which makes it possible to consider the use of this wavelength as the most preferable.

2019_04_843-847.pdf690.48 KB

Experimental study of synthetic polymeric materials as a basis in the creation of the advaced carrier matrix for the cultivation of limbal stem cells

Year: 2019, volume 15 Issue: №2 Pages: 495-501
Heading: Ophtalmology Article type: Original article
Authors: Karpovich V.V., Kulikov A.N., Churashov S.V., Chernysh V.F., Grigoriev S.G., Blinova M.I., Nashchekina Yu.A., Aleksandrova O.I., Khorolskaya Yu.I., Nikonov P.O., Tsobkallo E.S., Moskalyuk O.A., Melnikov A.S., Serdobintsev P.Yu., Mashel' T.V., Pisugina G.A., Perepletchikova D.A., Khoroshikh D.A.
Organization: Institute of Cytology, Russian Academy of Sciences, St. Petersburg Polytechnic University, St. Petersburg State University, St. Petersburg State University of Industrial Technology and Design

Purpose: to study in the experiment the properties of three diferent types of synthetic polyester matrices, to conduct their comparative assessment and determine the optimum as a carrier for the cultivation and transplantation of limbal stem cells while eliminating limbal insufciency. Material and Methods. Transparency, mechanical properties (strength, elongation at break, modulus of rigidity), biocompatibility with cell cultures of the cornea, as well as the study of the timing of matrix biodegradation in vivo were carried out. Results. The study examined the optical and mechanical properties of matrices made from polylactide-glycolide (PLG), polylactide-caprolactone (PLC) and poly-e-caprolactone (PCL). It was experimentally shown that human and rabbit limbal stem cells, as well as human corneal epithelium cells, adhered on the surface of all types of matrices under investigation, and during cultivation they retained the typical structure of actin cytoskeleton, the ability to proliferate and migrate. Diferences in the interaction of diferent cell cultures with diferent types of carriers were revealed. The terms of biodegradation of PLC matrices with a thickness of 5 μm was about 30 days. Conclusion. The results indicate that it is possible to use 5 μm thick PLC matrices as a carrier of cultured limbal stem cells.

2019_02-1_495-501.pdf1.03 MB