Saratov JOURNAL of Medical and Scientific Research

Neuroamines — regulators of local processes at bone marrow autotransplantation

Year: 2015, volume 11 Issue: №4 Pages: 524-526
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Vorobyova O.V.
Organization: Chuvash State University n.a. I.N. Ulyanov

Purpose: to determine the content of neuroamines (histamine, serotonin, catecholamines) in bioamine structures of bone marrow after autotransplantation. Material and methods. Animals were injected into the tail vein of bone marrow suspension obtained from the femur of the same mouse. It was taken from the femoral bone marrow of 1 ml and placed in 2 ml of physiological saline and thoroughly stirred. 1 ml of bone marrow suspension was injected into the tail vein. Cryostat sections were treated with luminescent-histochemical methods. Results. After bone marrow autotransplantation marked changes were observed in the neurotransmitters of the bone marrow — an increasing number of granular luminescent cells decrease in the amount of granules in them, and a decrease in the number of mast cells because of their degranulation. A weak luminescence was determined in nuclei in neutrophils. Perhaps this proves the activation of the immune response. Conclusion. The bone marrow autotransplantation leads to a redistribution of the structures of the bone marrow of histamine, catecholamines and serotonin, which changes the direction of cytodifferentiation, the content of neuroamines in granular luminescent cells and mast cells (cells of autonomic regulation).

1. Semenov E.V., Stancheva N.V., Bondarenko S.N. Quality of patients with lymphoma in different terms after high-dose chemotherapy with autologous hematopoietic stem cell transplantation. Clinical.oncohematology: Basic research and clinical practice 2014; 7 (4): 577-582
2. Abedi М., Foster ВМ., Wood KD., et al. Haematopoietic stem cells participate in muscle regeneration. Br J Haematol. 2007; 138 (6): 792-801
3. Lyubovtseva E.V. Bioaminsoderzhaschie structure of the bone marrow in systemic blood diseases. Morphology 2012, 3: 95-96
4. Makarenkova V.P., Coast N.V., Schurin M.R. The system of dendritic cells: their role in the induction of immunity in the pathogenesis of infectious diseases, autoimmune diseases and cancer. Immunology 2002; 2: 68-76
5. Stavinskaya О.А. The role of histamine and serotonin in the maintenance of immune homeostasis. In: National Conference "Allergology and Clinical Immunology: interdisciplinary problems". Allergic Russian magazine 2008; 1: 283-284
6. Karkischenko N.N., Grachev S.V. Guide to laboratory animals and alternative models in biomedical technologies. M.: Medicinein, 2010; 344 p.
7. Cross SAM, Ewen SWB, Rost EWDA. Stindi of the methods available for the cytochhem: cal localization of histamine by fluorescence induced with ophthalaldehyde or acetadehude. J. Histochem 1971; 6: 471-476
8. Gordon B.M. Mathematical justification specificity lyuminestsentno- histochemical reactions autolyuminestsentnyh structures. Morphology and histochemistry of normal tissue, pathology and exsperimente 1982; 2: 71-76.

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