Saratov JOURNAL of Medical and Scientific Research

histamine

The role of histaminergic system in the effects of skin flap autotransplantation on microcirculation at absolute insulin deficiency

Year: 2020, volume 16 Issue: №2 Pages: 521-525
Heading: Рathophysiology Article type: Original article
Authors: Antipova O.N., Lagutina D.D., Stepanova T.V., Savkina A.A., Kuznetsova N.A., Andronova Т.А., Kiriyazi T.S, Ivanov A.N.
Organization: Saratov Medical University "Reaviz", Saratov State Medical University
Summary:

The objective of the article is to estimate the impact of the histaminergic system in the effects of skin flap autotrans-plantation (SFA) on microcirculation in white rats with alloxan insulin deficiency. Material and Methods: The studies were carried out in 60 white rats, divided into 3 groups: 20 control intact animals, 20 animals with alloxan diabetes — the comparison group, 20 animals with alloxan diabetes and SFA— the experimental group. The mechanisms of blood circulation modulation and perfusion of posterior paw dorsal skin, the concentration of histamine in the blood serum, and morphological changes in the SFAarea were evaluated. Results: The significant increase of skin perfusion on 43%, redistribution of microcirculation modulation active mechanisms and the increase in histamine serum concentration in 2,5 times underthe influence of SFA were observed in rats of the experimental group. Conclusion: The distant stimulating effect of SFA on microcirculation in conditions of absolute insulin deficiency is realized with increasing histamine serum concentration. Moreover, the stimulation of the blood flow regulation active mechanisms by increasing the histamine concentration is not equivalent — the effects on endothelium-dependent and neurogenic regulation mechanisms prevail, and the activation of histamine smooth muscle cell receptors is much less pronounced.

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Neuroamines — regulators of local processes at bone marrow autotransplantation

Year: 2015, volume 11 Issue: №4 Pages: 524-526
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Vorobyova O.V.
Organization: Chuvash State University n.a. I.N. Ulyanov
Summary:

Purpose: to determine the content of neuroamines (histamine, serotonin, catecholamines) in bioamine structures of bone marrow after autotransplantation. Material and methods. Animals were injected into the tail vein of bone marrow suspension obtained from the femur of the same mouse. It was taken from the femoral bone marrow of 1 ml and placed in 2 ml of physiological saline and thoroughly stirred. 1 ml of bone marrow suspension was injected into the tail vein. Cryostat sections were treated with luminescent-histochemical methods. Results. After bone marrow autotransplantation marked changes were observed in the neurotransmitters of the bone marrow — an increasing number of granular luminescent cells decrease in the amount of granules in them, and a decrease in the number of mast cells because of their degranulation. A weak luminescence was determined in nuclei in neutrophils. Perhaps this proves the activation of the immune response. Conclusion. The bone marrow autotransplantation leads to a redistribution of the structures of the bone marrow of histamine, catecholamines and serotonin, which changes the direction of cytodifferentiation, the content of neuroamines in granular luminescent cells and mast cells (cells of autonomic regulation).

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