Saratov JOURNAL of Medical and Scientific Research

Physiology and Pathophysiology

Peculiarities of microcirculatory reactions after subcutaneous implantation of polycaprolactone matrices mineralized by vaterite

Year: 2018, volume 14 Issue: №1 Pages: 35-41
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Norkin I.A., Ivanov A.N., Kurtukova M.O., Savelyeva M.S., Martyukova A.V., Gorin D.A., Parakhonsky B.V.
Organization: Saratov State Medical University, Saratov state university, Skolkovo Institute of Science and Technology
Summary:

The aim is to assess skin microcirculation changes arising during subcutaneous implantation of polycaprolactone scaffolds mineralized by vaterite. Material and Methods. The experiment was carried out on 30 albino rats divided into two groups: a negative control group and an experimental one. We implanted polycaprolactone scaffold with exhausted foreign protein subcutaneously into rats of the negative control group. We implanted polycaprolactone matrix mineralized by vaterite subcutaneously into the animals of the experimental group. Research methods include laser Doppler flowmetry and morphological examination of the tissues of the matrix implantation area. Results. Changes of skin microcirculation over the matrix allocation area correspond to the morphological pattern of tissue reactions. Biocompat-ibility disorders take the form of inflammation in the scaffold implantation area that is accompanied by stable perfusion rise associated with local bloodstream modulation changes. We did not observe inflammation signs in the surrounding scaffold tissues during implantation of polycaprolactone matrices mineralized by vaterite. Besides local microcircula-tory reactions possess a transient character disappearing completely by the 21st day after the implantation. Conclusion. Complex of the given functional and morphological studies allow us to ascertain high-grade biocompatibility of polycaprolactone matrices mineralized by vaterite thus giving rise to prospect of their use for tissue regeneration stimulation.

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The modified assessment technique of the condition of lungs at the experimental animals at extreme exposures

Summary:

Purpose: to develop a modified method of measuring a set of biophysical indices of the lungs elaborated, which allows to assess the extent of their lesions from mild, moderate to severe. This technique allows for a short time to conduct a rapid assessment of the lungs of a large number of experimental animals of different species under varying extreme influences for the subsequent extrapolation of these data to humans. Material and Methods. The study was conducted using Chinchilla rabbits weighing 2.5-3.0 kg, and sheep weighing 25-35 kg. Lung mass and volume were defined, and values of biophysical indices were calculated: specific density of air-filled and collapsed lungs; their ratio — ALPHA index; residual volume. Results. The values of main lung biophysical indices of experimental animals in norm were received. The scale of lung condition estimate and degree of injury of experimental animals as compared with the control group was developed. Conclusion. The given technique of rapid estimate of the lung condition according to biophysical indices allows to give a rapid and objective assessment of significant lung injuries in experimental animals. Easy realization and availability of necessary equipment enable to use this technique in the studies of experimental lung pathology.

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Investigation of the relationship between changes in thermographic and flowmetric parameters of skin peripheral hemodynamics in laboratory rats

Year: 2017, volume 13 Issue: №4 Pages: 901-907
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Datsenko A.V., Fomina T.V., Dyoshin I.A., Kazmin V.I.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to determine the quantitative patterns and gradations of the degree of manifestation of changes in IRT indicators, depending on LDF data, corresponding to different conditions and peripheral blood flow disorders in the skin of laboratory rats. Material and Methods. Microcirculation of blood in bio-objects in background studies and immediately after the experimental modeling of hypobaric hypoxia was determined with the help of LDF, while simultaneously performing remote dynamic infrared thermography of the rats'tail skin. A comparison of LDF and IRT data was carried out using statistical methods of correlation-regression analysis. Results. The decrease in tissue blood flow was accompanied by a decrease in temperature, with an increase in perfusion of blood along the microcirculation pathways, the temperature of the skin surface increased. The quantitative criteria of experimental diagnostics in the form of boundary values and ranges of temperature indicators for an estimation of a different degree of severity of violations of the microcirculation of blood (peripheral hemodynamics) are determined. With a decrease or increase in tissue blood flow to 10, 10-25, 25-40 and more than 40% of the baseline (background control), the negative or positive skin surface temperature increase was up to 0.6, 0.6-1.2, 1.2-1.8 and more than 1.8°C respectively. Conclusion. Results of thermal imaging can be used as a criterion for predictive assessment of the state and changes in cutaneous peripheral blood flow in laboratory rats.

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Exotoxin A Pseudomonas aeruginosa influence on working efficiency of white rats

Year: 2017, volume 13 Issue: №3 Pages: 472-474
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Popovich V.I., Vasyutina V.O., Morrison A.V.
Organization: Central Scientific Research Experimental Institute 33, Saratov State Medical University
Summary:

Objective: to study exotoxin A Pseudomonas aeruginosa influence on physical and mental efficiency of white rats. Material and Methods. The experiments were carried out on white rats following intraperitoneal injections of exotoxin A Pseudomonas of different doses (0.1,0.5 and 1.0 LD50). Physical and mental efficiency of white rats was determined by the scheme of "Machfs camera". Results. Introducing exotoxin A Pseudomonas of 1 LD50 there was conditioned response damage in 20% of animals. The amount of cases of physical incapacity introducing highly effective doses of exotoxin A Pseudomonas overcomes the amount of cases of reaction destruction (44%). Conclusion. Exotoxin A Pseudomonas aeruginosa does not reveal a selective effect on the central nervous system and significant influence on physical efficiency of white rats.

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Different reaction of core histones H2A and H2B to the red laser radiation

Year: 2017, volume 13 Issue: №3 Pages: 469-472
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Brill G.E., Egorova A.V., Bugaeva I.O., Ushakova O.V., Matyushkina O.L.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Aim: to investigate the influence of red laser irradiation on the processes of self-assembly of core histones H2A and H2B. Material and Methods. Solutions of human histone proteins were used in the work. Self-assembly was studied by the method of wedge dehydration. Image facies analysis consisted in their qualitative characterization and calculation of quantitative indicators with subsequent statistical processing. Results. It was established that linearly polarized laser light of the red region of the spectrum (A=660 nm, 1 J/cm2) significantly modifies the process of self-assembly of core histone H2B, while the structure of the facies of H2A histone changing to a lesser extent. Conclusion. Red laser radiation influences on the on the processes of self-assembly of core histones H2A and H2B. There is a differential sensitivity of different classes of histones to laser action. Histone proteins used in the experiments are present in the form of aqueous salt solutions. Red light realizes the effect seems to be due to the formation of singlet oxygen by direct laser excitation of molecular oxygen.

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Systemic inflammatory response and cytokine profile at burn injury in dynamics

Year: 2017, volume 13 Issue: №2 Pages: 229-232
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Bozhedomov A.Yu., Simonyan M.A., Morrison A.V.
Organization: Leningrad Regional Clinical Hospital, Saratov State Medical University
Summary:

The aim of the article is to study the dynamics of indices of systemic inflammation in correlation with the following factors: the outcome of the disease, disturbances in proinflammatory cytokines rate and vascular endothelial dysfunction markers rate at burn injury in dynamics. Material and Methods. 152 patients with burn injuries over 30 units by Frank's index were under the study. The research on the level of interleukins, endothelial vessels growth factor, macrophage chemotactic protein, level of endothelin and C-reactive protein was performed with quantitative immuno-enzymatic detection. The amount of circulating of desquamated endothelial cells was accounted by phase-contrast microscopy. Results. Proinflammatory cytokines rate and vascular endothelial dysfunction markers rate significantly increase reaching their maximum indices to the 7-15th day of burn injury and indicating the expressiveness of systemic inflammatory response syndrome. Conclusion. The received data have become important in the determination of increased number of criteria of systemic inflammatory response syndrome depending on the outcomes of the burn injury and endothelial dysfunction development.

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Acousto-optical method of blood typing: the comparison of application of anti-A and anti-B monoclonal antibodies with standard hemagglutinating sera

Year: 2017, volume 13 Issue: №1 Pages: 022-028
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Doubrovski V.A., Medvedeva M.F.
Organization: Saratov State Medical University
Summary:

The aim of the article is a comparison of resolution of the acousto-optical method for blood typing for two types of reagents: monoclonal antibodies and standard hemagglutinating sera. Materials and Methods: The influence of the concentrations of reagents (monoclonal antibodies and standard hemagglutinating sera), of blood sample, which is to be tested, as well as of the duration of the ultrasonic action on the biological object upon the resolution of acousto-optical method were investigated. The peculiarity of this work is the application of digital photo images processing by pixel analysis previously proposed by the authors. Results: The optimal experimental conditions to obtain maximum of the resolution of the acousto-optical method were found, it creates the prerequisites for a reliable blood typing. Conclusions: The present paper is a further step in the development of acousto-optical method for determining human blood groups.

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Microgliocyte argentation on histological sections of hippocampus of rabbits

Summary:

Purpose: to obtain morphological data to identify microglia on histological sections of hippocampus of rabbits using silver salt impregnation. Material and methods. Histological sections of hippocampus of rabbits impregnated with salt deposited silver. Using the Rio Hortega method for detecting fibrous glia, choose the desired chemical composition and acid-base parameters of the silver solution and reducing agent for the detection of microglia. Results. In histological sections of the hippocampus revealed microglia contrast with branched spikes and cells without shoots with thin flagellums. Conclusion. In visiting the experiments carried out in natural conditions, it is crucial to conduct biomedical research in the shortest possible time. We need to get not only the functional results of research, but also by their morphological, substantiating the data. The present method of impregnation microglia can be used as a rapid method for the morphological study of cerebral blood flow and neural tissue hypoxia.

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Use of a remote infrared thermography in experimental medicine at extreme influences

Summary:

The study presents an analysis of published data on the use of remote infrared thermography in medicine and veterinary science and evaluated the main opportunities, methods and ways of thermovision registration in experimental medical studies on biological objects, including at extreme influences. The following resources of the bibliographic electronic databases had been used: eLibrary, PubMed, Clinical Key, Science. Now methods of infrared thermography in insufficient degree are used in experimental medicine, including when studying adverse biological effect of various harmful and dangerous extreme factors, which main manifestation, especially in the early period after exposure, are systemic microvascular disorders, determining the state of capacity of human biomodels. These dynamic remote ther-mographic studies of experimental biological objects can be used to evaluate disorders of the peripheral circulation, working capacity and an emotional condition of the biomodels exposed to extreme factors, including early after experimental studies in laboratory and field work.

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Cells identification and counting in blood native state on the basis of digital microscopy.

Year: 2016, volume 12 Issue: №4 Pages: 549-555
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Doubrovski V.A., Zabenkov I.V, Torbin SO, Tsareva O.E.
Organization: Saratov State Medical University
Summary:

The research goal is to develop an algorithm for the processing of photo images of native blood samples to determine the concentration of erythrocytes, leukocytes and platelets without individual separate preparation of cell samples. Materials and Methods. The objects of investigation were the samples of the whole donated blood, diluted 400 times by saline. Special "photo templates", the effect of "highlighting" of leukocytes, which was detect by authors, and the resolution of platelets from leukocytes by the areas of their photo images were suggested for identification of the cells. Results. 80 photo images of native blood solutions were selected for computer processing, while the total number of cells counted was: erythrocytes — 4184, platelets — 292 and leukocytes — 84, total — 4560 blood cells. Comparison of the results achieved with ones obtained by "manual" account or by the device for formed elements counting Sysmex XT-400i gives satisfactory results. Conclusion. It is shown that the accuracy of counting of the native blood cells may be comparable with the accuracy of similar studies by means of smears. At the same time the proposed analysis of native blood simplifies greatly the samples preparation in comparison to smears, permits to move from the detection of blood cells ratios to the determination of their concentrations in the sample.

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