Saratov JOURNAL of Medical and Scientific Research


Peritoneal fluid cells reactivity in modeling of aseptic inflammation

Year: 2020, volume 16 Issue: №3 Pages: 810-813
Heading: Рathophysiology Article type: Original article
Authors: Godovalov А.Р., Morozov I.A., Karpunina T.N.
Organization: Perm State University of Medicine n.a. Academician Ye.A. Vagner of Ministry of Health of the Russian Federation

The aim of investigation was to evaluate the characteristics of the response of rat peritoneal leukocytes and mast cells (MC) when using experimental aseptic inflammation. Material and Methods: Rats of the 1st group (n=7) were intra-peritoneally injected with 1 % thioglycolate broth, animals ofthe2nd(n=7)— the same broth, diluted 1:10. Control group rats (n=8) received an equal volume of saline. After removing animals from the experiment in the peritoneal fluid, the number of leukocytes was counted and their viability was evaluated. MC degranulation was detected by toluidine blue technique. All MCs were divided according to the degree of degranulation. Results: A model of aseptic inflammation of the peritoneum was reproduced. It was found that the number of leukocytes of peritoneal fluid, especially the number of non-viable cells, increases by more than 2 times with an increase in the concentration of the eluting agent (p<0.05). In experimental animals, the number of MCs with signs of degranulation increased to 89.5±2.2% compared with their level in the control group (49.0±3.9%; p<0.05). Conclusion: With the introduction of thioglycolate broth into the rats peritoneal cavity, an adequate model of inflammation is reproduced, suitable for studying the activity of different types of cells and humoral factors. The clinical picture of inflammation varies depending on the degree of degranulation of MC, which confirms their participation in the development of the pathological process.

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Neuroamines — regulators of local processes at bone marrow autotransplantation

Year: 2015, volume 11 Issue: №4 Pages: 524-526
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Vorobyova O.V.
Organization: Chuvash State University n.a. I.N. Ulyanov

Purpose: to determine the content of neuroamines (histamine, serotonin, catecholamines) in bioamine structures of bone marrow after autotransplantation. Material and methods. Animals were injected into the tail vein of bone marrow suspension obtained from the femur of the same mouse. It was taken from the femoral bone marrow of 1 ml and placed in 2 ml of physiological saline and thoroughly stirred. 1 ml of bone marrow suspension was injected into the tail vein. Cryostat sections were treated with luminescent-histochemical methods. Results. After bone marrow autotransplantation marked changes were observed in the neurotransmitters of the bone marrow — an increasing number of granular luminescent cells decrease in the amount of granules in them, and a decrease in the number of mast cells because of their degranulation. A weak luminescence was determined in nuclei in neutrophils. Perhaps this proves the activation of the immune response. Conclusion. The bone marrow autotransplantation leads to a redistribution of the structures of the bone marrow of histamine, catecholamines and serotonin, which changes the direction of cytodifferentiation, the content of neuroamines in granular luminescent cells and mast cells (cells of autonomic regulation).

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Clinical and diagnostic importance of changes of colon at chronic prostatitis

Year: 2010, volume 6 Issue: №2 Pages: 453-457
Heading: Urology Article type: Original article
Authors: V.M. Popkov
Organization: Saratov State Medical University

The aim of researches was studying clinical, microbiological and morphological characteristic of colon at patients at chronic prostatitis, definition of method of pathogenetic therapy on the basis of the received results.
Material and methods of investigation. 50 patients at chronic bacterial prostatitis, 50 patients at asymptomatic inflammatory prostatitis and 30 practically healthy males were inspected. Microflora of prostata's secret and colon, morphology and structure of components of diffuse neuroendocrine system of colon were studied. Clinical, microbiological, иммуногистохимические methods and morphometrical analysis were applied.
Results. It is defined, that at 74% patients with asymptomatic inflammatory prostatitis irritable bowel syndrome and at 26% - chronic nonulcerative colitis were diagnosed. At all patients at chronic bacterial prostatitis chronic nonulcerative colitis were detected. These variants were correlleted with different types of intestinal dysbiosis and degree of microbe producing of prostate. Use probiotic Bactistatin® at patients with a chronic prostatitis raises clinical efficiency of antibacterial therapy, promotes reduction of inflammatory changes, restoration of its microbic landscape and neuroendocrine homeostasis of colon.
inclusion. At chronic prostatitis structural and functional pathology of colon are often registered, they are connected with clinical variant of prostatitis and can mask of prostata's pathology. Using Bactistatin® at patients with a chronic prostatitis is proved and effective

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Year: 2008, volume 4 Issue: №2 Pages: 132-136
Heading: Stomatology Article type: Original article
Authors: S.A. Vostrikova, A.V. Lepilin, Ya.G. Karabushina,, E.K. Makletsova
Organization: Saratov State Medical University, Saratov Stomatologic Clinic «Denta-Med»

Results oftreatment ofpatients with periodontitis using ultrasonic system "Vector"or sealer and Grace! curetes were compared. Microflora of periodontal pockets and gingival mastcells were studied. It is shown that the use of ultrasonic system “Vector" has clinical morphological efficiency and allows to achieve stable remission of periodontitis during 6 months of watching at 91,2% patients. After periodontitis treatment with use of sealer and Gracei curetes functional activity of mast cells was increased and relapse of periodontitis at 30% patients developed.

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