Saratov JOURNAL of Medical and Scientific Research

Accelerating regeneration of eye socket bone defect by modifying xenogenic elastin biomaterial

Year: 2021, volume 17 Issue: №2 Pages: 350-356
Heading: Тhematic supplement Article type: Original article
Authors: Lebedeva A.I., Shangina O.R., Nigmatullin R.T., Gareev E.M., Kutushev R.Z.
Organization: Bashkir State Medical University of the Russian Health Care, Russian Eye and Plastic Surgery Center

Purpose: to identify the morphological aspects of the replacement of transplanted preserved and modified xenogenic elastin biomaterials in the rat eye socket defect. Material and Methods. Wistar rats were given a 7x4 mm defect in the upper wall of the eye socket. Wistar rats were treated with a 7x4 mm defect in the upper edge of the orbit. In the first experimental group (n=30), a preserved xenogenic elastin biomaterial (pXEBM) was placed in the defect zone, and in the second experimental group (n=30), a modified porous xenogenic elastin biomaterial (mXEBM) was placed. In the control group (n=30), soft tissues were sutured in layers. The tissues were excised after 1, 3, 6 and 12 months. Histological methods were used. Results. Grafts have osteoinductive, osteoconductive properties and are replaced by a full-fledged bone regenerate. After implantation, the mXEBM was replaced 2 times fasterthan the pXEBM. In the con- trol group, after 1 year, the bone defect did not spontaneously recover. Conclusion. Xenogenic biomaterials based on elastin are ultimate biomimetics. The porosity of the graft significantly accelerates the course of bone repair processes.

1. Echave MC, Burgo LS, Pedraz JL, et al. Gelatin as Biomaterial for Tissue Engineering. Curr Pharm Des 2017; 23: 3567-84. 10.2174/0929867324666170511123101.
2. Munhoz MAS, Pomini KT, Plepis AMG, et al. Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects. PLoS One 2020; 15 (4): e0231112. DOI: 10.1371/journal, pone. 0231112.
3. Benerji A. Medical statistics in clear language. M.: Practicheskaya medicina, 2007; 287 p.
4. Willeke FD, Quaglinob D. Signaling pathways in elastic tissues. Cellular Signalling 2019; 63: 109364. URL: https:// cellsig. 2019.109364.
5. Lebedeva Al, Nigmatullin RT, Kutushev RZ. Morphological aspects of restoration of the rat eye socket defect by biomaterial based on elastin. Saratov Journal of Medical Scientific Research 2020; 16 (2): 614-8.
6. Basalyga DM, Simionescu DT, Xiong W, et al. Elastin degradation and calcification in an abdominal aorta injury model: role of matrix metalloproteinases. Circulation 2004; 110: 3480-7.
7. Yu Q, Stamenkovic I. Cell surface-localized matrix metalloprotein-ase-9 proteolytically activates TGF-beta and promotes tumor invasion and angiogenesis. Genes Dev 2000; 14: 163-76.
8. Komaki H, Tanaka T, Chazono M, et al. Repair of segmental bone defects in rabbit tibiae using a complex of beta-tricalcium phosphate, type I collagen, and fibroblast growth factor-2. Biomaterials 2006; 27: 5118-26.
9. Chalmers J, Gray DH, Rush J. Observations on the induction of bone in soft tissues. J Bone Joint Surg Br 1975; 57 (1): 36-45.
10. IvanovAA, DanilovaTI, Popova OP, etal.The periosteum as a source of progenitor osteogenic cells for the restoration of resorbed alveolar bone. Russian Journal of Stomatology 2016, 4: 39-42.
11. Ngai D, Lino M, Bendeck MP Cell-Matrix Interactions and Matricrine Signaling in the Pathogenesis of Vascular Calcification. Front Cardiovasc Med 2018; 5: 174.
12. Kutushev RZ, Nigmatullin RT, Musina LA, etal. Anatomical and biomechanical justification of the graft for reconstructive surgery of the bone walls of the eye socket. Saratov Journal of Medical Scientific Research 2020; 16 (2): 610-4.
13. Polezhaev LV. Regeneration by induction. Moscow: Medicine, 1977; 184 p.
14. Crespo AR, Da Rocha АВ, Jotz GP, et al. Increased serum sFas and TNFalpha following isolated severe head injury in males. Brain Inj 2007; 21 (4): 441-7. DOI: 10.1080/02699050701311125.
15. Ragnoni E, Palombo F, Green E, et al. Coacervation of a-elastin studied by ultrafast nonlinear infrared spectroscopy PhysChem Chem Phys2016; 18: 27981-90.
16. McCarthy B, Yuan Y, Koria P. Elastin-like-polypeptide based fusion proteins for osteogenic factor delivery in bone healing. Biotechnol Prog 2016; 32 (4): 1029-37. DOI: 10.1002/btpr. 2269.
17. Sinha A, Vyavahare NR. High-glucose levels and elastin degradation products accelerate osteogenesis in vascular smooth muscle cells. Diab Vase Dis Res 2013; 10 (5): 410-9. DOI: 10.1177/1479164113485101.

2021_02-1_350-356.pdf1.21 MB

No votes yet