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Antibacterial activity of biosynthetized silver nanoparticles against Pseudomonas aeruginosa

Year: 2025, volume 21 Issue: №1 Pages: 100-103
Heading: Pharmacology, clinical pharmacology Article type: Original article
Authors: Kupryashina M.A., Ponomareva E.G., Mamonova I.A., Kul'shan' Т.А.
Organization: Saratov State Medical University, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, Russia
Summary:

Objective: to analyze the antibacterial effect of bioformed silver nanoparticles on the museum and clinical strain of Pseudomonas aeruginosa in vitro. Material and methods. Silver nanoparticles were obtained using metabolites of the non-pathogenic bacterium Azospirillum brasilense SR80. The size, shape, homogeneity and degree of aggregation of the obtained nanoparticles were characterized by electron microscopy, UV-visible spectroscopy and dynamic light scattering. The antibacterial effect of various concentrations of silver nanoparticles was assessed by the level of metabolic activity of cells using the resazurin test. Results. Biosynthesized silver nanoparticles exhibited antibacterial activity against the reference strain P. aeruginosa ATCC 27853 and the clinical strain P. aeruginosa. A decrease in the metabolic activity of bacterial cells was shown, depending on the concentration of nanoparticles. Maximum antibacterial activity (100% cell death) for the reference strain P. aeruginosa was achieved when the cells were treated with silver nanoparticles at a concentration of 12,5 ug/ml, for the clinical strain, complete suppression of cell growth was noted when adding nanoparticles at a final concentration of 25 ug/ml. Conclusion. Silver nanoparticles obtained by the "green" synthesis method using the cell-free extract of Л. brasilense SR80 exhibit pronounced concentration-dependent antimicrobial activity against the clinical and standard strains of P. aeruginosa in vitro.

Keywords: silver nanoparticles, Pseudomonas aeruginosa, Azospirillum brasilense, antibacterial activity, "green" synthesis

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