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The condition of the intracellular metabolism of peripheral blood leukocytes in arterial hypertension (experimental study)

Year: 2023, volume 19 Issue: №4 Pages: 404-409
Heading: Рathophysiology Article type: Original article
Authors: Romanova Т.Р., Bugaeva I.O., Uvarova I.A., Perevoznikova T.V., Shapoval O.G.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky, Saratov State Medical University
Summary:

Objective: the comparative analysis of the features of intracellular metabolism of polymorphonuclear leukocytes (PNL) in the peripheral blood of rats with the normal blood pressure and hereditary stress-induced arterial hypertension (ISIAH). Material and methods. Indicators of carbohydrate and lipid metabolism, activity of some key redox enzymes in leukocytes of rats as well as a level of PNL functional activity were examined by histochemical methods. Results. The study determined that the stable increased blood pressure is accompanied by hypertrophy of the left ventricle of the heart, degenerative changes in cardiomyocytes and the decreased microcirculatory bed density. In this case amount of intracellular glycogen in leukocytes was decreased in 23% at unchanged amount of intracellular lipids. Activities of intracellular ATP-ase and myeloperoxidase were reduced in 22% with non-significant increasing of succinate dehydrogenase activity (in 13%). Amount of PNL with positive NBT-test was increased in 35% in the rats of ISIAH. Conclusions. Analysis of the studied metabolic indicators of PNL shows disturbance of the energy-supplying and enzymatic processes in the leukocytes on the background of arterial hypertension.

Keywords: arterial hypertension, intracellular metabolism, polymorphonuclear leukocytes

Bibliography:
1. Zhou В, Carrillo-Larco RM, Danaei G, et al. Worldwide trends in hypertension prevalence and progress in treatment and control from 1990 to 2019: A pooled analysis of 1201 population-representative studies with 104 million participants. Lancet. 2021; 398 (10304): 957-80. DOI:10.1016/S0140-6736 (21) 01330-1
2. Sabirov IS, Murkamilov IT, Fomin VV, et al. Arterial hypertension at a young age: The current state of the problem. The Scientific Heritage. 2021; (72-2): 15-23.
3. Kosova VYu, Medvedev IN. Modern view on the epidemiology, pathogenesisand classification of arterial hypertension. Bulletin of Science and Education. 2019; 9-1 (63): 87-90.
4. Shabalin AV, Khodykina EN, Sentyakova TN. Oxidative metabolism of polymorphonuclear leukocytes in hypertension in patients of different ages. Siberian Scientific Medical Journal. 2007; (6): 78-83.
5. Vaskina ЕА. Arterial hypertension: oxidative stress and endothelial dysfunction. DSc abstract. Novosibirsk, 2004; 36 p.
6. Tong S, Neboori HJ, Tran ED, Schmid-Schonbein GW. Constitutive expression and enzymatic cleavage of ICAM-1 in the spontaneously hypertensive rat. J Vase Res. 2011; 48 (5): 386-96. DOI:10.1159/000323474
7. Nesterova IV, Chudilova GA, Kovaleva SV, et al. Methods for a comprehensive assessment of the functional activity of granulocytes in normal and pathology. Guidelines for immunol-ogistsallergists, physicians and biologists of clinical laboratory diagnostics. Krasnodar, 2017; 51 p.
8. Romanova ТР. Method of modeling intracerebral hema-tomas in arterial-rial hypertension. Pathological Physiology and Experimental Therapy. 1989; (3): 80-81.
9. Romanova ТР, Arkhangelsky AV Early morphofunctional changes in blood and myocardium with stress against the background of arterial hypertension. Collection of scientific works for the 100th anniversary of L. S. Schwartz. Moscow, 2003: p. 122-7.
10. Romanova ТР, Uvarova IA. Cardio- and cerebrovascu-lar changes under the influence of different types of a stress at arterial hypertension. Bulletin of Medical Internet Conferences. 2014; 4 (6): 946.
11. Romanova ТР, Uvarova IA. Morphofunctional changes in the vessels of the microvasculature of the myocardium of hypertensive rats under stress. Izvestia of Saratov University. New series. Series: Chemistry. Biology. Ecology. 2017; 17 (2): 208-11. DOI: 10.18500/1816-9775-2017-17-2-208-211
12. Attaeva MJ, Gurizheva MV, Vasilenko VM. Stimulated tet-razolium activity of neutrophils in patients with chronic pyelonephritis complicated by arterial hypertension. Modern Problems of Science and Education. 2017; (5): 38.
13. Boltaev DE, Madaminov AS. Comparative study of the indicators of lysosomal cationic proteins in patients with urolithiasis. Bulletin of Emergency Medicine. 2011; (2): 29-31.
14. Magomedova KM, Arslanova RM, Osmanova РМ, et al. Modern ideas about the structure and mechanisms of functioning of succinate hydrogenase. International Student Scientific Bulletin. 2020; (3): 146.
15. Bucket GG, Khrapova MV, Dushkin Ml. Features of lipid and glucose metabolism of the hypertensive line of NISAG rats. Siberian Scientific Medical Journal. 2013; (4): 5-11.
16. Petrukhin IS, Lunina EYu. Actual problems of prevention of cardiovascular diseases in Russia. Upper Volga Medical Journal. 2012; 10 (1): 3-8.
17. Kostin IV, Shangina ОА, Shelikhov VG. Metabolic therapy in cardiology from the standpoint of evidence-based medicine. Fundamental and Clinical Medicine. 2021; 6 (1): 60-8. DOI:10.23946/2500-0764-2021 -6-1 -60-68

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