Saratov JOURNAL of Medical and Scientific Research

Peculiarities of microcirculatory reactions in the area of experimental wound defect in white rats

Year: 2022, volume 18 Issue: №2 Pages: 272-275
Heading: Рathophysiology Article type: Original article
Authors: Loyko D.D., Savkina А.А., Stepanova T.V., Kiriiazi T.S., Osnovin O.V., Andronova Т.А., Abdrahmanova I.I., Fedorov A.N., Ivanov A.N.
Organization: Saratov State Medical University

Objective: to reveal changes of microbloodflow parameters by the laser Doppler flowmetry method in the process of wound defect healing and possibility of their application to modernization of the technologies forwound healing agents effectiveness evaluating. Material and methods. The studies were carried out on 25 white rats divided into 2 groups: 10 control intact rats, 15 animals with a full-thickness experimental skin defect. The microcirculatory parameters of the wound skin edges in rats were evaluated with laser Doppler flowmetry. Histological assay of the wound defect tissues were also performed. Results. It was established that changes in skin microcirculation at the wound defect edges were characterized by inflammatory hyperemia, which was manifested by 27% perfusion increase and increase of normalized amplitudes of myogenic, respiratory and cardiac oscillations. Changes in microcirculation are verified by the morphological picture of inflammation, which reflects an increase in the number of full-blooded vessels of the arterial and venous bed, as well as leukocyte infiltration of the bottom and edges of the wound. Conclusion. Monitoring of microcirculatory disorders occurring in the area of skin wounds allows us to assess the dynamics of the reparative process, which can be used to develop and evaluate the effectiveness of existing drug and non-drug methods of stimulating regeneration.

1. Sen CK. Human wound and its burden: updated 2020 compendium of estimates. Adv Wound Care (New Rochelle) 2021; 10 (5): 281-92.
2. Haller HL, Sander F, Popp D, et al. Oxygen, pH, lactate, and metabolism-how old knowledge and new insights might be combined for new wound treatment. Medicina (Kaunas) 2021; 57 (11): 1190.
3. Yousefi S, Qin J, Dziennis S, et al. Assessment of microcirculation dynamics during cutaneous wound healing phases in vivo using optical microangiography. J Biomed Opt 2014; 19 (7): 76015.
4. Sorensen MA, Petersen LJ, Bundgaard L, et al. Regional disturbances in blood flow and metabolism in equine limb wound healing with formation of exuberant granulation tissue. Wound Repair Regen 2014; 22 (5): 647-53.
5. Kulikov DA, Glazkov AA, Kovaleva YuA. et al. Prospects of laser Doppler flowmetry application in assessment of skin microcirculation in diabetes. Diabetes Mellitus 2017; 20 (4): 279-85.
6. Smotrin SM, Dovnar Rl, Vasil'kov AYU, et al. Effect of a dressing containing gold or silver nanoparticles on experimental wound healing. Journal of the Grodno State Medical University 2012; 1 (37): 75-80.
7. Humeau A, KoTtka A, Abraham Р, et al. Time-frequency analysis of laser Doppler flowmetry signals recorded in response to a progressive pressure applied locally on anaesthetized healthy rats. Phys Med Biol 2004; 49 (5): 843-57.
8. Bi H, Feng T, Li B, et al. In vitro and in vivo comparison study of electrospun pla and pla/pva/sa fiber membranes for wound healing. Polymers (Basel) 2020; 12 (4): 839.
9. Krupatkin Al. Blood flow oscillations — new diagnostic language in microvascular research. Regional Blood Circulation and Microcirculation 2014; 13 (1): 83-99.
10. Tecchio С, Cassatella MA. Neutrophil-derived cytokines involved in physiological and pathological angiogenesis. Chem Immunol Allergy 2014; (99): 123-37.
11. Wang Z, Qi F, Luo H, Xu G, et al. Inflammatory microenvironment of skin wounds. Front Immunol 2022; (13): 789274.

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