Characteristics of electrophoretic mobility of red blood cells in patients on long-term hemodialysis
Year: 2021, volume 17 Issue: №3 Pages: 453-457
Heading: Internal Diseases Article type: Original article
Authors: Boldyreva E.S., Kudryavtsev E.V.
Organization: Izhevsk State Medical Academy
Heading: Internal Diseases Article type: Original article
Authors: Boldyreva E.S., Kudryavtsev E.V.
Organization: Izhevsk State Medical Academy
Summary:
Objective: to analyze the electrophoretic properties of erythrocytes in patients receiving programmed hemodialysis treatment. Material and methods. The study included 100 patients with an established diagnosis of chronic kidney disease, stage 5D, receiving treatment with chronic (long-term) hemodialysis. Control group was composed of 17 healthy blood donors. Evaluated indicators of the electrophoretic activity of erythrocytes: vibration amplitude, proportion of motile cells, ellipticity and aggregation index. Results. In comparison with the control group, there were revealed significant changes in the amplitude of oscillations and the proportion of motile cells (p<0.001). No significant gender-defined differences were observed (p>0.05). Significant age differences between young adults and senior adults groups (p=0.048) were observed in the average vibration amplitude, and ellipticity between the middle-aged and senior (p=0.040), old and senior (p=0.035) adults groups. According to the duration of hemodialysis treatment, a decrease in the amplitude of fluctuations to the maximum from the minimum period was revealed: less than 1 year (p=0.029); 1 year (p=0.035), the indicators of the aggregation index have the highest values in the groups with the minimum (p=0.044) and maximum (p=0.035) periods of dialysis. Conclusion. In patients on long-term hemodialysis, the amplitude of red blood cells oscillations is significantly reduced with strong decreasing tendency associated with long duration of chronic hemodialysis treatment.
Bibliography:
1. Herzog CA, Asinger RW, Berger AK, et al. Cardiovascular disease in chronic kidney disease. A clinical update from Kidney Disease: Improving Global Outcomes (KDIGO). Kidney International 2011; 80 (6): 572-86.
2. Levin A, Stevens P, Bilous R, et al. KDIGO 2012 Clinical Practice Guideline for the evaluation and management of Chronic Kidney Disease. Kidney International Supplements 2013; 3 (1): 1-136.
3. Carracedo J, Alique M, Vida C, et al. Mechanisms of cardiovascular disorders in patients with chronic kidney disease: a process related to accelerated senescence. Frontiers in Cell and Developmental Biology 2020; (8): 185.
4. Martusevich AK, Martusevich AA, Deriugina AV, et al. Electrophoretic study of erythrocyte membranes under the action of reactive oxygen species and nitric oxide. Biomedicine 2019; 15 (1): 102-12.
5. Deryugina AV, Martusevich AA, Khlamova YuN, et al. Electrophorhetic mobility of erythrocytes in inflammation. Medical Newsletter of Vyatka 2016; 4 (52): 57-60.
6. Deryugina AV, Ivashchenko MN, Ignatiev PS, et al. Alterations in the phase portrait and electrophoretic mobility of erythrocytes in various diseases. Modern Technologies in Medicine 2019; 11 (2): 63-8.
7. Deryugina AV, Martusevich AA, Antipenko AA, et al. Effect of chronic stress on electrokinetic properties and oxidative metabolism of erythrocytes. Bioradicals and Antioxidants 2015; 2 (2): 26-35.
8. Obukhova LM, Erlykina EL, Kopytova TV, et al. The role of membrane lipids in forming the electrokinetic properties of red blood cells in solid tumors and psoriasis. Kazan Medical Journal 2015; 96 (5): 764-8.
9. Kazakova IA, levlev YN. Electrophoretic mobility of erythrocytes in patients with chronic renal disease of 5th dialysis stage with different level of arterial pressure. Therapy 2019; 5 (2): 30-5.
10. Krylov VN, Deryugina AV, Antipenko EA. The electrophoretic mobility of red blood cells as a way to assess the function of the adrenal cortex in stress and pathological states of the organism. Yu.A. Ovchinnikov bulletin of biotechnology and physical and chemical biology 2013; 9 (2): 39-42.
11. Olszewska M, Bober J, Wiatrow J, et al. The impact of hemodialysis on erythrocyte membrane cytoskeleton proteins. Advances in Hygiene & Experimental Medicine 2015; (69): 165-75.
12. Tukin VN, Fedorova MZ. Age-dependent changes in biochemical indices of blood and their relationship with the stiffness of membranes of hemocytes in the blood of healthy men and women. Belgorod State University Scientific Bulletin 2012; 18 (3): 155-60.
13. Kazantsev AV, Suyetenkov DYe, Andronov EV, et al. Gender features of rheological properties of blood (plasma viscosity, aggregation and deformation of erythrocytes) in patients with chronic generalized periodontitis. Saratov Journal of Medical Scientific Research 2014; 10 (1): 56-61.
14. Baskurt О, Meiselman Н. Erythrocyte aggregation: basic aspects and clinical importance. Clinical Hemorheology and Microcirculation 2013; 53 (1-2): 23-37.
15. Podzolkov VI, Koroleva TV, Pisarev MV, et al. Abnormal microcirculation and red blood cell function as a cardiovascular risk factor in metabolic syndrome. Rational Pharmacotherapy in Cardiology 2018; 14 (4): 591-7.
| Attachment | Size |
|---|---|
| 2021_03_453-457.pdf | 263.97 KB |
Русский
English