Method for modeling a skin wound in pigs in experiment
Year: 2021, volume 17 Issue: №1 Pages: 46-50
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Bogdanov S.B., Karakulev A.V., Bogdanova Yu.A., Sotnichenko A.S., Gilevich I.V., Melkonyan K.I., Alad’ina V.A.
Organization: Research Institute — Regional Clinical Hospital No. 1 n. a. Professors. V. Ochapovsky, Kuban State Medical University
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Bogdanov S.B., Karakulev A.V., Bogdanova Yu.A., Sotnichenko A.S., Gilevich I.V., Melkonyan K.I., Alad’ina V.A.
Organization: Research Institute — Regional Clinical Hospital No. 1 n. a. Professors. V. Ochapovsky, Kuban State Medical University
Summary:
Aim: development of an experimental model for studying the course of the wound process and the effectiveness of applying wound dressings both in clean conditions and on a purulent wound in one animal. Material and Methods. Pig wound modeling is used as an in vivo model. In the operating room, under general anesthesia in the lateral position, the laboratory animal is marked with the future burn surface. A hot steel contact pad applies a thermal injury to the skin of a given size and the required depth of thermal injury (IIIB and IV grades according to Vishnevsky’s classification). Then necrectomy was performed with primary and delayed plasty using the studied wound dressings. After this, non-viable tissues are removed with primary and delayed autodermoplasty and plastic wound dressings. The research subject — in vivo study included 4 Landrace pigs (animal weight from 18 to 24 Kg). Results. With burns IIIB Art. under the decellularized scaffolds, scar tissue was formed by the 12th day, under the recellularized ones by the 8th day, epithelialization of the wound occurred. With burns IV Art. granulation tissue without aseptic inflammation on the 8th day was formed. Conclusion. The developed method for modeling a wound in an experiment allows one animal to analyze the engraftment of wound coverings, both in early and in staged surgical treatment of burns.
Bibliography:
1. Ostrovsky NV, Kuspits EV. Problems of organization of first aid and interaction with a specialized burn center. In: New technologies of medical care. Materials of the scientific and practical conference. Moscow, 2016; 22-3.
2. Bogdanov SB, Afaunova ON. The use of wound coverings in the surgical treatment of borderline burns of the extremities in functionally active areas. Vrach-aspirant 2016; 79 (6): 4-9.
3. Climov М, Medeiros Е, Farkash ЕА, et al. Bioengineered self-assembled skin as an alternative to skin grafts. Plastic and Reconstructive Surgery Global Open 2016; 4 (6): e731.
4. MacNeil S. Progress and opportunities for tissue-engineered skin. Nature 2007; 445 (7130): 874-80.
5. Burd A, Ahmed K, Lam S, et al. Stem cell strategies in burns care Burns 2007; 33 (3): 282-291.
6. Chua A. WC, Khoo YC, Tan BK, et al. Skin tissue engineering advances in severe burns: review and therapeutic applications. Burns Samp; trauma 2016; 4 (1): 3.
7. Leclerc T, Thepenier C, Jault P, et al. Cell therapy of burns. Cell proliferation 2011; (44): 48-54.
8. Golinski P, Menke H, Hofmann M, et al. Development and characterization of an engraftable tissue-cultured skin autograft: alternative treatment for severe electrical injuries. Cells Tissues Organs 2014; 200 (3-4): 227-39.
9. Zeller N, Valesky E, Butting M, et al. Clinical application of a tissue-cultured skin autograft: an alternative for the treatment of non-healing or slowly healing wounds? Dermatology 2014; 229(3): 190-8.
10. Bogdanov SB, Babichev RG, Marchenko DN, et al. Plastic surgery with full-layer skin autografts of various etiologies of the Russian Academy of Sciences. Innovative medicine of the Kuban 2016; (1): 30-7.
11. Climov М, Medeiros Е, Farkash ЕА, et al. Bioengineered self-assembled skin as an alternative to skin grafts. Plastic and Reconstructive Surgery Global Open 2016; 4 (6): e731.
12. Keck M, Haluza D, Lumenta DB, et al. Construction of a multi-layer skin substitute: simultaneous cultivation of keratinocytes and preadipocytes on a dermal template. Burns 2011; 37 (4): 626-30.
13. Wormald JC, Fishman JM, Juniat S. Regenerative medicine in otorhinolaryngology. J Laryngol Otol 2015; 129 (8): 732-9.
14. Gallico GG 3rd, O'Connor NE, Compton CC, et al. Permanent coverage of large burn wounds with autologous cultured human epithelium. New England Journal of Meditsine 1984; 311 (7): 448-51.
15. Van der Veen VC, van derWal MB, van Leeuwen MC, et al. Biological background of dermal substitutes. Burns 2010; 36 (3): 305-21.
16. Shevchenko RV, JamesSE, Reed MJ, et al. Pork experimental model as an effective tool for transferring scientific knowledge to the clinic to replenish the combustiologist's arsenal. Combustiology 2007;(30)URL:http://combustiolog.ru/journal/svinaya-e-ksperimen-tal-naya-model-kak-e-ffektivny-j-instrument-perenosa-nauchny-h-znanij-v-kliniku-dlya-popolneniya-arsenala-kombustiologa/(26 Jan 2020).
17. Andreev SV. Modeling of diseases. Moscow: Medicine, 1973; 336 p.
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