Saratov JOURNAL of Medical and Scientific Research

Morphological aspects of restoring a rat's eye socket defect with elastin-based biomaterial

Year: 2020, volume 16 Issue: №2 Pages: 614-618
Heading: Ophtalmology Article type: Original article
Authors: Lebedeva A.I., Nigmatullin R.T., Kutushev R.Z.
Organization: Bashkir State Medical University of the Russian Health Care, Russian Eye and Plastic Surgery Center

The purpose of the study is to identify morphological aspects of replacement of xenogenic decellularized elastin matrix (DXEM) transplanted into a bone defect of the upper wall of the rat's eye socket. Materials and Methods. The experiment was performed on 60 Wistar rats, which produced a 7x4 mm defect in the upper edge of the orbit. DXEM was placed in the defect zone, in the experimental group (n=30). The size was similar to the defect and was fixed with a suture material-silk 50 microns. Soft tissues were sutured in layers in the control group (n=30). Tissue excision was performed after 1, 3 and 12 months. Histological, immunohistochemical and electron microscopic methods were used. Results. DXEM was gradually replaced by bone tissue against the background of a pronounced reaction of CD68+/MMP-9+ macrophages. This indicated its resorption and lysis. Osteogenesis occurred endesmally, periostally, which was preceded by centripetal migration of endothelial kidneys with subsequent differentiation into hemocapillaries and growth of loose fibrous connective tissue with progenitor cells. The microenvironment could contribute to differentiation of progenitor cells in the osteogenic direction and osteogenesis represented by reticulin fibers, TGFb, sulfated glycosaminoglycans in the control group, the defect remained open throughout the experiment. Conclusion. Decellularized biomaterial based on elastin matrix has osteoconductive and osteoinductive characteristics and can serve as an adequate biomimetic for bone defects restoration.

1. Sjostrom M, Sennerby L, Nilson H, et al. Reconstruction of the atrophic edentulous maxilla with free iliac crest grafts and implants: a 3-year report of a prospective clinical study. Clin Implant Dent Relat Res 2007; 9 (1): 46-59.
2. Joshi A. An investigation of post-operative morbidity following chin graft surgery. Brit Dent J 2004; 196 (4): 215-8.
3. Xenogenic biomaterial for regenerative surgery: Patent №2440148/Muldashev ER, Nigmatullin RT, Galimova VU, et al.; priority 21.12.2009; publ. 20.01.2012, Bull. 2.
4. Sevastyanov VI. Biocompatibility. Moscow, 1999; 367 p.
5. Benyon RC, Iredate JP Is liver fibrosis reversible? Gut 2000; 46: 443-6.
6. Van Ginderachter JA, Movahedi K, Hassanzadeh Ghassabeh G, et al. Classical and alternative activation of mononuclear phagocytes: picking the best of both worlds for tumor promotion. Immunobiology 2006; 211 (6-8): 487-501.
7. Rachmiel A, Leiser Y. The molecular and cellular events that take place during craniofacial distraction osteogenesis. Plast Reconstr Surg Glob Open 2014; 2: e98.
8. Jia Y, Zhu Y, Qiu Sh, Xu J, et al. Exosomes secreted by endothelial progenitor cells accelerate bone regeneration during distraction osteogenesis by stimulating angiogenesis. Stem Cell ResTher2019; 10: 12.
9. Pavlova SV, Rozanova IA, Chepeleva EV, et al. Angiogenic potential of cardiac stem and mesenchymal stromal cells of rat bone marrow. Circulatory Pathology and Cardiac Surgery 2015; 19 (4-2): 77-84.
10. Ни ВТ, Chen WZ. MOTS-c Improves Osteoporosis by Promoting Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells via TGF-p/Smad Pathway. Eur Rev Med Pharmacol Sci 2018; 22 (21): 7156-63.
11. Ngai D, Lino M, Bendeck MP Cell-Matrix Interactions and Matricrine Signaling in the Pathogenesis of Vascular Calcification. Front Cardiovasc Med 2018; 5: 174.
12. Gurumurthy B, Bierdeman PC, JanorkarAV. Composition of elastin like polypeptide — collagen composite scaffold influences in vitro osteogenic activity of human adipose derived stem cells. Dental Materials 2016; 32 (10): 1270-80.

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