Saratov JOURNAL of Medical and Scientific Research

The role of histaminergic system in the effects of skin flap autotransplantation on microcirculation at absolute insulin deficiency

Year: 2020, volume 16 Issue: №2 Pages: 521-525
Heading: Рathophysiology Article type: Original article
Authors: Antipova O.N., Lagutina D.D., Stepanova T.V., Savkina A.A., Kuznetsova N.A., Andronova Т.А., Kiriyazi T.S, Ivanov A.N.
Organization: Saratov Medical University "Reaviz", Saratov State Medical University

The objective of the article is to estimate the impact of the histaminergic system in the effects of skin flap autotrans-plantation (SFA) on microcirculation in white rats with alloxan insulin deficiency. Material and Methods: The studies were carried out in 60 white rats, divided into 3 groups: 20 control intact animals, 20 animals with alloxan diabetes — the comparison group, 20 animals with alloxan diabetes and SFA— the experimental group. The mechanisms of blood circulation modulation and perfusion of posterior paw dorsal skin, the concentration of histamine in the blood serum, and morphological changes in the SFAarea were evaluated. Results: The significant increase of skin perfusion on 43%, redistribution of microcirculation modulation active mechanisms and the increase in histamine serum concentration in 2,5 times underthe influence of SFA were observed in rats of the experimental group. Conclusion: The distant stimulating effect of SFA on microcirculation in conditions of absolute insulin deficiency is realized with increasing histamine serum concentration. Moreover, the stimulation of the blood flow regulation active mechanisms by increasing the histamine concentration is not equivalent — the effects on endothelium-dependent and neurogenic regulation mechanisms prevail, and the activation of histamine smooth muscle cell receptors is much less pronounced.

1. Dedov II, SHestakova MV, Vikulova OK. Epidemiology of diabetes mellitus in Russian Federation: clinical and statistical report according to the federal diabetes registry. Diabetes Mellitus 2017; 20(1): 13-41.
2. Akanov ZhA, Sejdinova ASh, Zhunusbekova NZh, et al. Epidemiology of overweight and obesity as major factors risk of hypertension and type 2 diabetes in modern conditions. Vestnik KazNMU 2015; (4): 289-93.
3. Kulikov DA, Glazkov АА, Kovaleva YuA, et al. Prospects of laser doppler flowmetry application in assessment of skin microcirculation in diabetes. Diabetes Mellitus 2017; 20 (4): 279-85.
4. Rassohin AV. Tissue placental therapy. SPb.: ELBI-SPb, 2014; 208 p.
5. IvanovAN, Shutrov IE, Norkin IA. Skin flap autografting as a method of microcirculation biostimulation in the conditions of normal and impaired innervation. Regional blood circulation and microcirculation 2015; 14 (3): 59-65 p.
6. Solgalova AS, Soldatov VO, Pershina MA, Pokrovskaya TG. Piracetam and betahistine: possible mechanisms of endothelioprotection. Kursk scientific and practical bulletin Man and his health 2018; (2): 61-9. 7. Buxtiyarova IP, DrogovozSM, Shhekina EG. A study of the hypoglycemic properties of ralec on a model of alloxan diabetes in rats. Vestnik KazNMU 2014; (4): 301-4.
8. KrupatkinAI, Sidorov VV. Laser Doppler flowmetry of blood microcirculation: a guide for doctors. Moscow: Medicina, 2005; 256 p.
9. Popyhova ЕВ, Ivanov AN, Stepanova TV, et al. The relation of carbohydrate metabolism disorders and markers of endothelial dysfunction in animals with absolute insulin deficiency at biostimulation by autotransplantation of the skin flap. Saratov Journal of Medical Scientific Research 2019; 15 (2): 379-82.
10. Unuofin JO, Lebelo SL. Antioxidant Effects and Mechanisms of Medicinal Plants and Their Bioactive Compounds for the Prevention and Treatment of Type 2 Diabetes: An Updated Review. Oxid Med Cell Longev 2020; (13): 1356893.
11. Lespagnol E, Dauchet L, Pawlak-Chaouch M, et al. Early Endothelial Dysfunction in Type 1 Diabetes Is Accompanied by an Impairment of Vascular Smooth Muscle Function: A Meta-Analysis. Front Endocrinol (April 17, 2020). URL: https://
12. Feng W, Shi R, Zhang C, et al. Visualization of skin microvascular dysfunction of type 1 diabetic mice using in vivo skin optical clearing method. J Biomed Opt 2018; 24 (3): 1-9.
13. Marco GS, Colucci JA, Fernandes FB, et al. Diabetes induces changes of catecholamines in primary mesangial cells. Int J Biochem Cell Biol 2008; 40 (4): 747-54.
14. Watson AMD, Gould EAM, Penfold SA, et al. Diabetes and Hypertension Differentially Affect Renal Catecholamines and Renal Reactive Oxygen Species. Front Physiol 2019; (10): 309.
15. IvanovAN, Lagutina DD, Gladkova EV, etal. Mechanisms of distant stimulatory action of skin autotransplantation in cases of peripheral nerve injury. Russian Journal of Physiology 2018; 104(11): 1313-24.
16. Hramcova YuS, Artashyan OS, Yushkov BG, et al. The influence of mast cells on reparative regeneration of tissues characterized by various degrees of immune privilege. Cell and Tissue Biology 2016; 58 (5): 356-63.
17. Hattori Y, Hattori К, Matsuda N. Regulation of the Cardiovascular System by Histamine. Handb Exp Pharmacol 2017; (241): 239-58.

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