Saratov JOURNAL of Medical and Scientific Research

Identify of argyrophilic cells of astrocytic glia to determine the hypoxic changes in nerve tissue


Aim: identification of astrocytic glia cells impregnated to determine the hypoxic changes in nerve tissue. Material and methods. Condition of hypoxia was simulated in laboratory rats with "jar hypoxia". For the impregnation of histo-logical sections were used as a basis for the method of Rio-Gortega. Results. Silver impregnation method revealed several morphological forms of astrocytic cells in hypoxic changes in the nervous tissue of the brain. Conclusion. There were marked morphological brain astrocyte cell forms reflecting their functional state. Traced compensatory changes astrocytic cell depletion of these processes and the appearance of pathological morphological characters, up to the fragmentation and destruction of cells.

1. Roitbak A.l. Glia and its role in neural activity. Saint-Petersburg: Science, 1993; 352 p.
2. Hudoerkov R.M., Voronkov D.N. Quantification of neurons and glia by computer morphometry. Byull eksper boil and med 2010; 149(1): 109-112
3. Tolstuhina T.l., Flerov M.A. ATPase activity of neurons and glia in with cramps caused by picrotoxin. Questions med himii 1999; 45(2): 145-149
4. Kligunenko E.N., Dzyak L.A., Ploschenko Y.A., et al. Neuroprotection in anesthesia and intensive care. Medical emergency conditions 2008; 15(2): 28-37
5. Akmaev I.G., Grinevich V.V. From neuroendocrinology to neyroimmunoendokrinology. Byull eksper biol and med 2001; 131(1): 22-32
6. Cherny T.V. The use of quantitative EEG to assess the efficacy of neuroprotective effects on neuroglial level. Medical emergency conditions 2013; 52(5): 117-123
7. Droblenkov A.V., Naumov N.V., Monid M.V., et al. Response of cellular elements on rat cerebral circulatory hypoxia. Medical academic journal 2013; 13(4): 19-28
8. Romeys В. Microscopic technique. Moscow: Foreign Literature Publishing House, 1953; 718 p. Russian (Ромейс Б. Микроскопическая техника. М.: Иностранная Литература, 1953; 718 с). 9. Ivaschenko V.V., Kirpatovsky V.I. Features adaptogenic effect of sodium hypochlorite in acute hypoxia, physical activity and thiopental anesthesia in the experiment. Experimental and clinical urology 2012; (2): 24-27
10. Datsenko A.V., Kazmin V.I. Complex microscopic express-analysis for biomedical full-scale investigation. Saratov Journal of Medical Scientific Research 2013; 9(4): 805-808.

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