Saratov JOURNAL of Medical and Scientific Research

Pseudomonas aeruginosa exotoxin A-induced hepatotoxicity in dynamics: an animal model in white mice

Year: 2015, volume 11 Issue: №4 Pages: 526-529
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison A.V., Popovich V.I., Morrison V.V.
Organization: Central Scientific Research Experimental Institute 33, Saratov State Medical University

Purpose: to study biochemical indices of peripheral blood which show organs functional changes. Material and Methods. The experiments were carried out on white mice in dynamics development of pseudomonas aeruginosa caused by intraperitoneal injection of various dosage of exotoxin A. Results. The dosage-dependent disturbances in activity of transaminases, a-HBDH, in content of proteins, lipids and cholesterol were revealed. The received data have determined the hepatotoxic effect of pseudomonas aeruginosa exotoxin A on changes of biochemical blood markers of liver function. Conclusion. The expressed hepatotoxic effect of Pseudomonas aeruginosa exotoxin A has been determined during all the phases of experiment even at sublethal toxin dose.

1. Rudnov V.A., Belsky D.V., Dekhnich F.V., Research group of resuscitation department. Infections at Resuscitation departments in Russia: results of national multicentral research. Clinical microbiology and microbial chemotherapy 2011; 13 (4): 294-303
2. Reshedko G.K., Ryabkova E.L., Krechnikova O.l., et al. Resistency to antibiotics of gram-negative agents of nosocomial infections at resuscitation department in general hospitals of Russia. Clinical microbiology and microbial chemotherapy. 2008; (10): 96-112
3. Driscoll JA, Brody SL, Kollef MH. The epidemiology pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 2007; 67 (3): 351-368
4. Miyazaki S, Matsumoto T, Tateda K, et al. Role of exotoxin A in inducing severe Pseudomonas aeruginosa infections in mice. J Med Microbiol 1995; 43 (3): 169-175
5. Nikbin VS, Aslani MM, Sharafi Z, et al. Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iran J Microbiol 2012; 4(3): 118-123
6. Morrison V.V., Morrison A.V. Qualitative and quantitative changes of cellular composition of peripheral blood in experimental animals effected by Pseudomonas aeruginosa exotoxin. Fundamental research 2008; (4): 19-25
7. Моррисон А.В., Попович В.И, Моррисон В.В., Иммунотоксические эффекты экзотоксина A Pseudomonas aeruginosa у белых мышей. Успехи современного естествознания 2015; 1 (6): 966-968
8. Borkar DS, Fleiszig SM, Leong С, et al. Association between cytotoxic and invasive Pseudomonas aeruginosa and clinical outcomes in bacterial keratitis. JAMA Ophthalmol 2013; 131 (2): 147-153
9. Chiu CC, Chen HH, Chuang HL, et al. Pseudomonas aeruginosa exotoxin A-induced hepatotoxicity: an animal model in rats. J Vet Med Sci 2009; 71 (1): 1-8
10. Tafesse FG, Guimaraes CP, Maruyama T, et al. GPR107, a G-protein-coupled Receptor Essential for Intoxication by Pseudomonas aeruginosa Exotoxin A, Localizes to the Golgi and Is Cleaved by Furin. J Biol Chem 2014; 289 (35): 24005-24018
11. Alhazmi Allaa Pseudomonas aeruginosa: pathogenesis and pathogenetic mechanisms. Int J Biol 2015; 7 (2): 44-67
12. Morrison V.V., Kudin G.B., Nefedova N.A. The state of processes of lipid peroxidation and antioxidant system in experimental pseudomonas aeruginosa intoxication in dynamics. Anesthesiology and Emergency medicine 2000; 3: 41-43
13. Morrison V.V., Morrison A.V. The changes in lipid peroxidation and the state of antioxidant system in experimental Pseudomonas aeruginosa intoxication dynamics. In: Oxygen and antioxidants. Volgograd, 2009; Vol. 1. P. 91-92.

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