№3, 2013, volume 9

Method of the epidermal surface biopsy (ESB) with the adhesive compositions is the alter-native to the classic his-tological examination. Materials and Methods. In this study medical adhesive "Sulfacrylate" was used, small portions of which had been spread on an object glass, and then on different skin areas. To study the structural organization of the samples ESB the technique of optical coherence tomography (ОСТ) was used. Results. We obtained the pictures that visualize the structural organization of the different layers of the epidermis in lichen planus, hyperkeratosis, scabies and other skin diseases. Conclusion. This technique allows non-invasive measurement of high-precision structure of different layers of the epidermis, which can be useful both for research and for the practical dermatology.

Objective: to select the optimal, clinically and economacally effective immunocorrector for the treatment of immune-EBV — mononucleosis in children. Materials: 400 patients with primary Epstein — Barr virus infection aged 1 to 14 years. Results. The therapy by cycloferon of primary Epstein — Barr virus infection observed a statistically significant reduction in the length of stay of patients in hospital, on average, 3 bed-days. Inclusion of cycloferon, viferon in the treatment of infectious mononucleosis, reduces the severity and duration of symptoms of intoxication, the temperature reaction, hepatotoxicity, splenomegaly, lymphoproliferative syndrome. Against the background of cycloferon lower incidence of post-infection anemia was observed. Conclusion. The inclusion of immunocorrectors into therapeutic interventions in primary Epstein — Barr virus infection has been clinically and economically proved.

The purpose of the research was detection of markers of systemic inflammatory response syndrome together with levels of markers of gram-negative endotoxinemia in HIV-patients for evaluation of role of endotoxin in immune system hyperactivation and progression of disease. Material and methods. Evaluation of levels of endotoxin, sCD14, interleu-kin 1(3, TNF-a and C-reactive protein was done in 232 HIV-positive patients. LAL-test, ELISA and biochemical tests were used. Results. The statistically significant increase of levels of all markers of systemic inflammatory response which were investigated was detected in the group of HIV-positive patients, comparing to group of healthy individuals. Direct correlation of sCD14 level with most of markers of systemic inflammatory response was found. Increased level of sCD14 was associated with more prominent clinical and laboratory signs of immunosupression. Conclusion. These results confirm a role of endotoxin which is a marker of microbial translocation and highly active biological substance in pathogenesis of immune system hyperactivation and progression of HIV-infection.

Purpose is to study the clinical, laboratory and instrumental characteristics of the course of rotavirus infection in children up to 3 years in the acute phase and during the convalescence period. Material and methods. A clinical, laboratory and instrumental examination of 320 children up to 3 years with moderate to severe rotavirus infection. Results. The presence of long-term persistence of rotavirus recovering from rotavirus infection. Identified functional disorders of the digestive system in the acute period and the period of convalescence in children up to 3 years with rotavirus infection. Conclusion. For a long period (up to 3 months), almost half of patients may experience intestinal dysfunction, possibly related to the long-term persistence of rotavirus.

Objective: to study the causes and clinical manifestations of disease in children referred for hospitalization in children infectious diseases hospital in Saratov with a diagnosis of «acute flaccid paralysis». Material and methods: 157 children with the diagnosis on admission of the guide «acute flaccid paralysis». Conducted clinical examination and laboratory tests included a general analysis of cerebrospinal fluid, urine, virological examination of nasal swabs and faeces, with the definition of a serological ELISA method and RPHA immunoglobulins to influenza, rubella, and enterovirus, immunological study of blood, cerebrospinal fluid PCR, electromyography of the affected limbs. Results. 77 patients (49%) with the disease associated with the violation of the musculoskeletal system were registered. In the other cases revealed polyneuropathy was not of poliovirus etiology. Etiological nature of the disease could be explained by 54% of patients. In 37 (46%) patients the diagnosis was formulated in accordance with the severity of paralysis. One child was diagnosed with a vaccine-associated poliomyelitis. Conclusion. The diagnosis of «acute flaccid paralysis» used as administered requires a detailed interpretation in a hospital.

The aimofthe article is to provide assessment of the role of West Nile virus in the infectious pathology on the territory of the Saratov region. Material and methods. Clinical samples (blood, serum, cerebrospinal fluid, urine) taken from 27 patients with symptoms resembling West Nile fever, receiving treatment in infectious departments of hospitals in Saratov and the Saratov region were analyzed. Results. In 2012 forthe first time in the Saratov region 11 cases ofWest Nile fever were detected. Conclusion. Analysis of the current epidemiological situation on West Nile fever demonstrates the necessity of strengthening of prophylactic and counter-epidemic measures to prevent cases of this disease in the region.

The aim of the study is to estimate prophylactic and clinical efficacy of cycloferon concerning acute respiratory viral infection among military men in the period of formation of military units. Material and methods. 1300 military men were under observation in the period of formation of military units. There was calculated efficiency coefficient. Index of efficiency, and severity of disease, frequency of development and character of complications were established. Results. It is established that application of both prophylactic and clinical course of tablet of cycloferon in the period of seasonal increase of morbidity of acute respiratory viral infection in the period of formation of military units enables to increase significantly efficiency of prophylactic and clinical activities, to decrease morbidity, frequency of severe and complicated forms of disease. Conclusion. According to these results, the perspectives of cycloferon use in prophylaxis and treatment of acute respiratory viral infection in the closed units were worked out.

The aim: to search the possible ways for increase of individual occlusive splints»s protective properties. Material and methods: 42 occlusive splints have been made with reinforced protective characteristics by the original technique. Results. Study of the level of comfort of splints and estimation of periodontal tissues proved the need for individual splints with increased protective properties. Conclusion. The individual splints should be made with reinforced protective properties for the long-term use.

Objective: to determine the level of dental health in children and adolescents living in the Saratov region. Material and methods. To assess the intensity and prevalence of major dental diseases 1510 patients have been examined by the method recommended by the WHO Collaborating Centre in Russia. Results. A comparative analysis of caries and occurrence of different types of teeth-maxillary anomalies among children aged from 3 to 18 years. Conclusion. The epidemiology of major dental diseases in the Saratov region is characterized by the significant spread of caries from 70 to 94,6% in the different age groups. The proportion of people with malocclusion among the surveyed reaches 46,6%.

Research objective is to increase quality of diagnostics and treatment of patients with a pain dysfunction syndrome of temporomandibular joint by the choice of diagnostic and medical actions. Material and Methods: 172 patients with a syndrome of pain dysfunction of temporomandibular joint have been underthe survey. All patients have been examined according to the scheme including subjective, objective and express methods. Results of the research allowed to reveal that at 30% of patients the syndrome of pain dysfunction of temporomandibular joint has been caused by disk dislocation. Upon termination of the treatment which has been carried out by the scheme, the normalization of relationship of joint elements has been observed. Conclusion: When the diagnosis of syndrome of pain dysfunction of temporomandibular joint is proved, it is necessary to apply complex treatment.