Endothelial dysfunction in the simulation of experimental periodontitis in white rats

The aim of the present work is to assess the dynamics of markers of endothelial dysfunction in the blood of white rats with experimental periodontitis at 3 and 5 weeks. Material and methods. The studies were carried out on 60 rats, divided into the following groups: control, which included 20 intact rats, and two comparison groups: the first — 20 animals with experimental periodontitis (3 weeks); the second — 20 animals with a ligature model of periodontitis (5 weeks). The concentration of markers of endothelial dysfunction was determined by enzyme-linked immunosorbent assay. Results. In white rats, against the background of experimental periodontitis, the concentrations of markers of inflammatory activation and markers of impaired endothelial vasomotor function significantly increase. At the 5th week of the experiment, the animals showed an increase in the concentrations of sE-selectin and VEGF — 2.5 times, asymmetric dimethylarginine and endothelin-1 was higher than the values of animals in comparison group 1 by 1.2 and 1.5 times, respectively. Conclusion. The modeling of experimental periodontitis in white rats was associated with the signs of inflammatory activation of the endothelium and its dysfunction, which was manifested by an increase in the concentration of blood of its markers at 3rd week of the experiment. In the period from the 3rd to the 5th week, despite the absence of a ligature, restoration of normal concentrations of endothelial dysfunction markers in the bloodstream were not found in animals with experimental periodontitis, and the progression of the endothelial vasomotor function disorders was observed.