Saratov JOURNAL of Medical and Scientific Research


Comparative characteristics of microcirculatory changes in white rats during subcutaneous implantation tests of polycaprolactone scaffolds containing vaterite and hydroxyapatite

Year: 2019, volume 15 Issue: №1 Pages: 98-103
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Chibrikova Yu.A., Kustodov S.V., Tyapkina D.A., Bugaeva I.O., Norkin IA.
Organization: Saratov State Medical University

Objective: comparative evaluation of skin microcirculatory functional changes over the implantation area of polycap-rolactone scaffolds with mineral carriers: vaterite and hydroxyapatite. Material and Methods. The experimental study was performed on 43 rats divided into three groups: two test groups and one negative control group. Polycaprolactone PCL-scaffolds containing ovalbumine and of no biocompatibility were implanted into the rats of negative control group (n=15). The implantation of PCL- scaffolds containing vaterite under their skin was performed on rats of the first test group, and animals of the second test group were implanted with PCL-scaffolds containing hydroxyapatite. The method of laser Doppler flowmetry was used to study the microcirculatory flow. Results. The constant increase in the perfusion index has been observed in the area of non-biocompatibile scaffolds implantation. Thechanges in local skin blood flow modulation have been also detected. The transient character of local microcirculatory responses with total normalization of skin perfusion and its mechanisms has been noticed by the 21st day of the trial following PCL-scaffolds with vaterite and hydroxyapatite implantation. Conclusion. The dynamic monitoring has allowed finding that mineralization of PCL-scaffolds with vaterite as well as hydroxyapatite leads to no prominent changes in skin perfusion at subcutaneous implantation tests on white rats suggesting the biocompatibility of these scaffold types.

2019_1_098-103.pdf1.07 MB

Study of Zn-hydroxyapatite crystals formation and growth on dentine base at ultrasonic influence

Year: 2017, volume 13 Issue: №2 Pages: 260-265
Heading: Stomatology Article type: Original article
Authors: Kazakova L.N., Tereschuk O.S., Kuligin A.V., Suetenkov D.Ye., Pichkhidze S.Ya., Garievskaya A.V.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov

The objective: the study analyses the features of spontaneous growth of hydroxyapatite crystals on dentinal substrate using (by the use of) artificially grown Zn-hydroxyapatite combined with ultrasonic treatment. Material and Methods. A dynamic study of dentinal substrate structure was conducted in a certain time interval after standard primary surface treatment of dentin micro-sections, divided into control and experimental groups. Dentin surfaces in the second group were covered by finely dispersed Zn-hydroxyapatite in combination with ultrasonic treatment. The investigation was performed by use of analytical complex based on scanning electron microscope with high resolution SEM Mira II LMU (Tescan) and energy dispersive analysis system. Results. There were revealed the structural changes of dentinal substrate surface overtime period due to directional growth of Zn-hydroxyapatite crystals. Conclusion. The conducted study showed the possibility of formation of the stable structures in the dentinal substrate when using Zn-hydroxyapatite in combination with ultrasonic treatment.

2017_02_260-265.pdf1.85 MB

Particle morphology of hydroxyapatite and its influence on the properties of biocomposite plasma coatings

Year: 2013, volume 9 Issue: №3 Pages: 441-445
Heading: Stomatology Article type: Original article
Authors: Melnikova I.P., Lyasnikova A.V., Lyasnikov V.N.
Organization: Saratov State Technical University

The purpose of the article is to identify patterns of change in the properties of biocompatible coatings during modernization of its structure by changing the morphology and crystallinity of the starting powder particles of hydroxyapatite (HA) for agglomeration and subsequent grinding. Material and methods. We investigated the morphology, degree of crystallinity and internal tension in HA powder with a particle size of 40-90 microns in the initial state and after the agglomeration process and structure piasmasprayed HA coatings application methods ray analysis (XRF and XRD) on DRON-3, infrared spectroscopy (FT-IR spectrometer Nicolet 6700), optical (MIM-8) and atomic force microscopy (SMM-2000), the laser microprobe (Spectrum 2000). Results: It was shown that change in particle morphology HA agglomerated and subsequently grinding increases the uniformity of the porous structure, its crystallinity, reduce internal stresses developing surface morphology of the coating and its nanostructuring. Conclusion. It is recommended for the improvement of characteristics of the porous structure (uniformity, strength, adhesion), and the surface morphology of implant to use agglomerating starting powders and their subsequent grinding.

2013-03_441-445.pdf264.02 KB

Scientific fundamentals for the development of dental implants

Year: 2013, volume 9 Issue: №3 Pages: 431-434
Heading: Stomatology Article type: Author's opinion
Authors: Lyasnikov V.N., Lepilin A.V., Protasova N.V.
Organization: Saratov State Technical University, Saratov State Medical University

The article deals with topical issues of development, production and application of modern biocompatible materials and coatings based on them to clinical practice. Particular attention is paid to the intraosseous dental implants, which considered to be the most popular and well established methods to address the defects of dentition with partial and fully edentulous state.

2013-03_431-434.pdf284.89 KB