Saratov JOURNAL of Medical and Scientific Research

Afanasieva G.A.

Saratov State Medical University n.a. VI. Razumovsky, Head of Department of Pathological Physiology, Assistant Professor, DSc.

Professor VitaliyViktorovich Morrison (to the birth anniversary)

Year: 2019, volume 15 Issue: №1 Pages: 124-126
Heading: anniversary Article type: Short message
Authors: Chesnokova N.P., Zavyalov A.l., Afanasyeva G.A.
Organization: Saratov State Medical University

The article is devoted to the 75th birth anniversary of Vitaliy Viktorovich Morrison, Professor and Merited Worker of Higher Education of the Russian Federation. Keywords: V.V. Morrison, anniversary.

2019_1_124-126.pdf450.5 KB

The teaching continuity of basic and clinical disciplines in the study of internal diseases

Year: 2016, volume 12 Issue: №3 Pages: 415-417
Heading: Pedagogics and Education Article type: Author's opinion
Authors: Ponomareva E.Yu., Rebrov А.Р., Afanasyeva G.A.
Organization: Saratov State Medical University

The article discusses the succession of teaching of basic and clinical (therapeutic) disciplines in higher medical school: the urgency and necessity of interdisciplinary cooperation, its form, expected results, existing obstacles and ways to overcome them. The experience cathedral groups in the implementation of the process and prospects of further interdisciplinary cooperation in teaching basic science education and internal medicine.

2016_03_415-417.pdf281.59 KB

The assessment of molecular markers of cell interaction and lipid peroxidation in rats with alloxan diabetes and trans- planted liver cancer after intravenous injection of gold nanorods

Year: 2015, volume 11 Issue: №2 Pages: 107-112
Heading: Biochemistry Article type: Original article
Authors: Bucharskaya А.В., Dikht N.I., Afanasyeva G.A., Terentyuk G.S., Zakharova N.B., Maslyakova G.N., Khlebtsov B.N., Khlebtsov N.G.
Organization: Institute of Biochemistry and Physiology of Plants and Microorganisms of Russian Academy of Sciences, Saratov State Medical University

Цель: оценить воздействие однократного внутривенного введения покрытых полиэтиленгликолем золотых наностержней на молекулярные маркеры межклеточного взаимодействия и показатели перекисного окисления в сыворотке крови четырех групп крыс: интактных, с аллоксановым диабетом, с перевитым раком печени; с перевитым раком печени на фоне аллоксанового диабета. Материалы и методы. Активность перекисного окисления липидов оценивалась по показателям гидроперекисей липидов, малонового диальдегида и молекул средней массы с использованием общепринятых спектрофотометрических методов, исследования молекулярных маркеров — фактора некроза опухоли TNF-a, инсулиноподобного фактора роста IGF-1, васкулоэн-дотелиального фактора роста VEGF-C проводились в сыворотке крови методом иммуноферментного анализа. Результаты. Выявлено, что при однократном внутривенном введении золотых наностержней не происходит значительного изменения количества маркеров межклеточного взаимодействия и показателей перекисного окисления липидов. в периферической крови. Заключение. Полученные результаты позволяют рекомендовать использование покрытых полиэтиленгликолем золотых наностержней в экспериментальной онкологии.

2015_02_107-112.pdf407.38 KB

Haemopoiesis and its regulation at various stages of haemopoietic cell differentiation of bone marrow

Year: 2012, volume 8 Issue: №3 Pages: 711-719
Heading: Physiology and Pathophysiology Article type: Review
Authors: Chesnokova N.P., Morrison V.V., Ponukalina Е.V., Zhevak Т.N., Afanasieva G.A., Polutova N.V., Newazhay Т.A.
Organization: Saratov State Medical University

Analysis of literature review has presented authors' opinion about regularities of consistent change of haemopoietic cell differentiation steps in bone marrow. Particular attention has been paid to the role of numerous cytokines (stem cell factor, fit-3ligand, interleukine-3, interleukine-4, interleukine-6, interleukine-7, tumor necrosis factor, colony stimulating factors, erythropoietin, thrombopoietin) in mechanisms of myelo-, erythro- and thrombocytopoiesis in the normal conditions. Results of investigation have showed the important significance of blood cytokine profile disturbances in pathogenesis of tumor progression in chronic lymphocytic leukaemia.

201203-711-719.pdf577.73 KB

Experimental and clinical application of laser doppler flowmetry in gastric and duodenal ulcerative bleedings

Year: 2011, volume 7 Issue: №4 Pages: 962-965
Heading: Surgery Article type: Original article
Authors: Kapralov S.V., Shapkin Y.G., Frolov I.A., Afanasieva G.A.
Organization: Saratov State Medical University

The research goal is to develop a new objective diagnostic method of prerecurrence syndrome that will prognose bleeding recurrence from gastroduodenal ulcers. Materials and methods. Method of laser Doppler flowmetry (LDF) of the regional perfusion of tissue has been used. The experimental part has been done on 30 white laboratory rats. Characteristics of regional tissue perfusion in the simulation and laser hemostasis of bleeding have been studied. Gastroduodenal endoscopy has been performed with laser Doppler flowmetry (ELDF) in clinical conditions to predict the recurrence of ulcerative bleeding. The prognostic method of gastroduodenal ulcerative bleeding was used in 58 patients hospitalized with such pathology and activity of bleeding Forrest II. Results. The study of microcirculation parameters and experimental hemostasis has showed the possibility of using LDF to measure its performance. Effective hemostasis has been accompanied by a significant decrease in perfusion. On the basis of microcirculation parameters in ulcerative bleeding, medical adrenaline test has been proposed for an objective verification pre-recurrence syndrome. To evaluate the effectiveness of endoscopic hemostasis perfusion has been measured before and after its implementation. Conclusion. ELDF has objectified the prognosis of ulcerative bleeding recurrence, verified pre-recurrence syndrome and evaluated the efficacy of endoscopic hemostasis

201104_962-965.pdf342.18 KB