Saratov JOURNAL of Medical and Scientific Research


Metal nanoparticles in treatment of experimental purulent wounds

Year: 2011, volume 7 Issue: №2 Pages: 530-533
Heading: Surgery Article type: Original article
Authors: Babushkina I. V.
Organization: Saratov Scientific Research Institute of Traumatology and Orthopedics

The research objective was to study the dynamics of the bacterial contamination and the healing rate of the experimental purulent wound when applying the suspension of copper, iron, zinc nanoparticles locally. Iron, copper and zinc nanoparticles received by a plasmochemical method were used under the influence of a plasmic stream with the temperature 5000-6000°K. 40 white rats — males with purulent wounds were used in the investigation. Planimetric and bacteriological methods of study helped evaluate the traumatic process. They were carried out on the 3rd, 5th, 7th, 10th and 14th days. Application of the suspension of copper nanoparticles ensured the elimination of the causative agent which had contaminated the wound in a short period of time in comparison with the groups in which iron and zinc nanoparticles had been applied and the control group. The shortest term of wound healing was detected when using the suspension of copper nanoparticles; a rather less healing rate was observed when using the suspension of zinc nanoparticles. Regenerating action of iron nanoparticles was less evident considerably, but significantly differed from the control group. In conclusion it was stated that the use of the suspensions of metal nanoparticles in the isotonic solution was proved to be effective in the local treatment of purulent wounds. Suppression of microbial flora and regenerative action were detected to various degrees. The most evident antiseptic and regenerative action was determined in copper nanoparticles

201102_530-533.pdf342.97 KB

Comparative study of antibacterial action of iron and copper nanoparticles on clinical Staphylococcus aureus strains

Year: 2010, volume 6 Issue: №1 Pages: 011-014
Heading: Biochemistry Article type: Original article
Authors: I.V. Babushkina, V.B. Borodulin, G.V. Korshunov, D.M. Puchinjan
Organization: Saratov State Medical University, Saratov Scientific Research Institute of Traumatology and Orthopedics

Research objective is to study antibacterial action of nanoparticles of iron and copper on polyantibiotically resistant clinical Staphylococcus aureus strains.
Materials and methods include antibacterial action of nanoparticles of copper and iron on 10 Staphylococcus au¬reus strains, isolated from patients with purulent complications stayed in the in-patient department of traumatology and orthopedics. Solutions of powders of iron and copper have been prepared directly before the experiment in concentra¬tion from 0,001 to 1 mg/ml.
it has been revealed that the influence of nanoparticles on growth of clinical strains and the intensity of antibacterial effect depends on the form of nanoparticles, their concentration and action time. concentration of 0,1 mg/ml and 1 mg/ ml of iron nanoparticles has provoked the decrease in quantity of microbe cells from 3 to 34 % (p <0,01). in smaller concentrations the reliable antibacterial effect has not been observed. Antibacterial activity of copper nanoparticles has been expressed in a wide range of concentrations from 0,001 mg/ml to 1 mg/ml, even during short-term action (30 minutes) it has provoked reduction of quantity of the microbe cells grown on the firm nutrient medium, 97-100 % in comparison with the control (p <0,001).
in conclusion it is to point out that copper nanoparticles have more expressed inhibitory effect on growth of clini¬cal strains of golden staphylococcus than iron nanoparticle suspension. inhibition degree depends on superdispersed powder dosage and incubation period

201001_011_014.pdf357.61 KB