Saratov JOURNAL of Medical and Scientific Research

antioxidant systems

Activity of processes of a lipoperoksidation at patients with a long postoperative pain syndrome against the combined application of application of low-intensive infrared laser therapy at impact on thymus area and pantovegin electrophoresis


The aim of the study was to optimize the treatment of postoperative gynecological patients using physiotherapy method. Material and methods. It was examined 220 patients in postoperative period. It was investigated antioxidant status and lipid peroxidation. Results. It is demonstrated that patients with prolonged postoperative pain disorders are more pronounced as compared with the control group. The article presents data on the effectiveness of the impact on the antioxidant system and lipid peroxidation in postoperative period of combined use of low-intensity infrared laser therapy when exposed area of the thymus and in pantovegin electrophoresis in patients sustained postoperative pain after gynecological laparotomy. Conclusion. It is shown that this method helps to reduce pain in the postoperative wound.

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Molecular and cellular mechanisms of destabilization of membranes and prenatal discharge of amniotic fluid

Year: 2013, volume 9 Issue: №4 Pages: 644-648
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Mikhaylov А.V., Dyatlova L.I., Chesnokova N.P., Ponukalina Е.V., Glukhova T.N.
Organization: Perinatal Centre, Saratov , Saratov State Medical University

Purpose: to establish the role of activation of lipid peroxidation and antioxidant deficiency of blood and amniotic fluid in the destabilization of membranes with early prenatal discharge of amniotic fluid. Material and methods. The analysis of lipid peroxidation products content in blood and amniotic fluid — diene conjugates (DC), malondialdehyde (MDA) and the quantitative determination of total peroxides (OxyStat), the estimation of the total antioxidant status (TAS), the activity of SOD and the content of ceruloplasmin in 20 pregnant with premature rupture of membranes in the gestation period of 22-34 weeks, were hospitalized at the Department of Pathology of Pregnancy of Saratov Perinatal Center in 2012. DC and MDA determination was carried out by conventional spectrophotometric methods of research, quantitative determination of peroxides (OxyStat) was carried out by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», 2008 using chemicals made by the company «Bender Medsystems»). The study of antioxidant status of serum and amniotic fluid was performed by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», England, 2008). Reagents («Bender Medsystems», Austria) were used to determine the total antioxidant status (TAS) and the SOD activity. To assess the content of ceruloplasmin reagent «Sentinel» (Italy) was used. Results. Indicators of lipid peroxidation in blood and amniotic fluid and anti-radical protection of biological membranes of cells and body fluids in the "mother-placenta-fetus" increased. Conclusion. Systemic activation of lipid peroxidation has been proved to be a
pathogenetic factor in the failure of membranes in prenatal discharge of amniotic fluid. The increase in the blood levels of lipid peroxidation products on the background of the activation of antioxidant system is considered as a prognostic sign of abortion.

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Current Aspects Of Estimation Of Bioradical Imbalance Of Patients With Multiple Sclerosis In The City Of Bryansk (Oxidant Stress And Multiple Sclerosis As An Example Of The Bryansk Population)

Year: 2009, volume 5 Issue: №4 Pages: 584-587
Heading: Neurology Article type: Review
Authors: N.P. Gribova, I.V. Khudyakova
Organization: Smolensk State Medical Academy

Article examines the role of free-radical oxidation of lipids and antioxidant systems in the development and characteristics of MS disease course. Oxidant-antiradical status was assessed in problem research laboratory of Clinical Biophysics, and antioxidant therapy Smolensk State Medical Academy with two direct methods of control - hemolumin-sention and radiospectroscopy and mathematical definition of the integral indexof the bioradical imbalance (coefficient K). Increase of degree EDSS was shown by activation of lipid peroxidation against background of tension in the antioxidant defense system, as well as free radical imbalance, which progressively intensified in direct proportion to rise on disease progression and the gravity of clinical manifestations of disease. Detected changes was determined by disease course and gravity of clinical manifestations of disease. Determined a direct correlation intensity of lipid peroxidation et antioxidant activity processes in patients with MS in ecologically unfavorable areas of the city of Bryansk

200904_584_587.pdf310.58 KB