Saratov JOURNAL of Medical and Scientific Research

Ivanov A.N.

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery of Saratov State Medical University n.a. V.I. Razumovsky, Department of Fundamental, Clinical and Experimental Studies, Head of Laboratory Diagnostics Department, Leading Research Assistant, Doctor of Medical Sciences

The relation of carbohydrate metabolism disorders and markers of endothelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap

Year: 2019, volume 15 Issue: №2 Pages: 379-38
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Popykhova E.B., Ivanov A.N., Stepanova T.V., Pronina E.A., Lagutina D.D.
Organization: Saratov State Medical University
Summary:

The purpose of this work is to study the relationship of carbohydrate metabolism disorders and markers of endo-thelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap. Material and Methods. The study was conducted on 60 male white outbred rats, which were divided into: an intact control group, a two comparative and an experimental group. In animals of the comparison group, alloxan diabetes mellitus (DM) was caused, in rats from the experimental group, an autotransplantation of the skin fap was performed against the background of developed alloxan diabetes. The levels of glycated hemoglobin (HbA1c), glucose, C-reactive protein (CRP) and vascular endothelial growth factor (VEGF) were determined. Results. Autotransplantation of the skin fap causes a normalization of the glycemic profle in animals of the experimental group, and also reduces subclinical infammation caused by hyperglycemia and stabilizes the expression of VEGF.Diabetes causes an increase in HbA1c by 69 % and CRP by 11 %, while аutotransplantation of the skin fap leads to a decrease in the concentration of HbA1c by 28 % and CRP by 7 %. Conclusion. Autotransplantation of the skin fap reduces the risk of microcirculatory disorders due to hyperglycemia and chronic subclinical infammation.

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Intense light deprivation as a factor in stress disorders of behavioral reactions and cognitive functions in the experiment

Year: 2019, volume 15 Issue: №2 Pages: 363-368
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Antipova O.N., Ivanov A.N., Zlobina O.V., Kirichuk V.F., Surovtseva K.A., Ankina V.D., Bondar G.D., Zenkina T.M., Polyukova M.V.
Organization: Saratov State Medical University
Summary:

Objective: to study the efect of light desynchronosis on behavioral reactions and cognitive functions of white male rats. Material and Methods. The study was conducted on 36 intact non-pedigree white male rats, which were divided into 2 groups: experimental and control. Animals were exposed to artifcial illumination 24 hours a day provided by the fuorescent lamp of 60 kW capacity. The same animals of the experimental group on the 10th, 21st and 30th days were tested in the installations «Dark-light camera» and «Open feld». Results. On the 10th day of intensive light deprivation, the indicators refecting the research-orientation activity reached the maximum values, which indicated the development of stress damage to the animal organism. On the 21st day of the experiment, the

Keywords: pneumocysts
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Comparative characteristics of microcirculatory changes in white rats during subcutaneous implantation tests of polycaprolactone scaffolds containing vaterite and hydroxyapatite

Year: 2019, volume 15 Issue: №1 Pages: 98-103
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Chibrikova Yu.A., Kustodov S.V., Tyapkina D.A., Bugaeva I.O., Norkin IA.
Organization: Saratov State Medical University
Summary:

Objective: comparative evaluation of skin microcirculatory functional changes over the implantation area of polycap-rolactone scaffolds with mineral carriers: vaterite and hydroxyapatite. Material and Methods. The experimental study was performed on 43 rats divided into three groups: two test groups and one negative control group. Polycaprolactone PCL-scaffolds containing ovalbumine and of no biocompatibility were implanted into the rats of negative control group (n=15). The implantation of PCL- scaffolds containing vaterite under their skin was performed on rats of the first test group, and animals of the second test group were implanted with PCL-scaffolds containing hydroxyapatite. The method of laser Doppler flowmetry was used to study the microcirculatory flow. Results. The constant increase in the perfusion index has been observed in the area of non-biocompatibile scaffolds implantation. Thechanges in local skin blood flow modulation have been also detected. The transient character of local microcirculatory responses with total normalization of skin perfusion and its mechanisms has been noticed by the 21st day of the trial following PCL-scaffolds with vaterite and hydroxyapatite implantation. Conclusion. The dynamic monitoring has allowed finding that mineralization of PCL-scaffolds with vaterite as well as hydroxyapatite leads to no prominent changes in skin perfusion at subcutaneous implantation tests on white rats suggesting the biocompatibility of these scaffold types.

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Sexual dimorphism of dynamics of concentration of endothelium alteration factors in blood in surgical treatment of chronic generalized periodontitis.

Summary:

Aim: to study the sexual dimorphism of the concentration in the blood of endothelial alteration factors in the dynamics of the surgical treatment of moderate chronic generalized periodontitis (CGP) in comparison with clinically healthy volunteers. Material and Methods. A total of 20 clinically healthy volunteers (average age is 30 (24; 37), 50% are men) and 31 patients with moderate chronic generalized periodontitis (average age is 31 (27; 38), 40% are men) were examined. Serum concentrations of homocysteine and C-reactive protein were determined. Patients with chronic generalized periodontitis were treated following the surgical scheme. Results. In patients with chronic generalized periodontitis, prior to treatment, an increased level of homocysteine and C-reactive protein (CRP) was detected in the serum. Conservative treatment in the preoperative period caused a decrease in the level of homocysteine in patients, while there was no significant change in the rate of C-reactive protein. 10 days after the surgery, an increase in the concentration of homocysteine and C-reactive protein in serum was observed in patients of both sexes, indicating a reactive change. At the same time, reactive changes were more pronounced in women. Long-term results of surgical treatment of chronic generalized periodontitis indicate a decrease in C-reactive protein and homocysteine levels in patients of both sexes. Sexual dimorphism was manifested by the fact that women had lower homocysteine content than men: 9.4 (8.9; 9.7) versus 13.3 (10.8; 14.1) umol/l (p=0,004). Conclusion. Patients of both sexes with moderate chronic generalized periodontitis have elevated C-reactive protein and homocysteine levels. Sexual dimorphism is manifested by higher levels of homocysteine, but not C- reactive protein in men. The acute period of surgical treatment of chronic generalized periodontitis is characterized by an increase in the concentration of factors affecting the endothelium in the blood, which are more pronounced in women. Long-term surgical treatment results of chronic generalized periodontitis demonstrate a decrease of homocysteine and C-reactive protein levels in blood with homocysteine levels being lower in women than in men.

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Morphofunctional state of spleening under light desynchronosis in the experiment

Year: 2018, volume 14 Issue: №3 Pages: 533-536
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Karetnikova A.Yu., Terekhina E.S., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

 Aim: to study the effects of light desynchronosis on the morphofunctional state of the spleen of white male rats. Material and Methods. The study of light desynchronosis was carried out on 48 male rats using the Light Light model with an illumination power of 300 Ix in the daylight and 500 Ix in the dark. The laboratory animals in the experiment were divided into four groups: the control and three experimental ones, in which they were exposed to light for 1 day, 10 days, 21 days respectively. In the course of morphometry of histological sections of the spleen, the following parameters were assessed: the area of white pulp lymphoid follicles, B- and T-clependent zones of white pulp, the number of germinative centers and immunoblasts in B- and T-zones. Results. On day 1 of the experiment, an increase in the area of the lymphoid follicle, periarterial and B-dependent zones, an increase in the number of immunoblasts and reproduction centers was registered. By the 10th day there was a partial restoration of these indicators relative to the control values. On the 21st day of the experiment, a decrease in the growth dynamics of all the morphometric parameters of the spleen was observed. Conclusion. Light desynchronosis induces by the Light Light model leads to the development of the pathology of the immune system with an extensive shift of all measurable indicators. Key words: morphofunctional state of the spleen, morphometric criteria, light desynchronosis, immunity.

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The correlation between structural changes in articular hyaline cartilage and the parameters of systemic manifestations of inflammatory reaction in the pathogenesis of early stages of primary osteoarthritis

Year: 2018, volume 14 Issue: №3 Pages: 518-523
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Gladkova Е.М., Ivanov A.N.
Organization:
Summary:

Aim: to study the correlations of structural disorders of articular hyaline cartilage with changes in concentration of pro- and anti-inflammatory cytokines at the early stages of osteoarthritis. Material and Methods. Ultrasound and MRI investigations of knee joints (T2*-relaxometry) were performed to 67 females of main group aged 35-50 with initial manifestations of (0-1 stages) of primary osteoarthritis and 30 conventionally healthy individuals. The level of interleukin-1 (3 was determined in blood serum with multi-parameter system MAGPIX (Luminex Corporation), Urine CTX-II was identified in urine and YKL- 40, interleukin-4 — in blood serum of the subjects by ELISA. Results. The levels of Urine CTX-II: 32.5 (30.9; 35.1) ng/ml, interleukin-1 (3: 6.09 (4.81; 7.69) pg/ml and YKL-4064.1 (58.1; 77.0) ng/ml of main group patients were higherthan in the comparison group. The correlation force between Urine CTX-II and cartilage glycoprotein YKL-40 (R=0.7); interleukin-1 (3 and cartilage glycoprotein YKL-40 (R=0.3) was lower; and the correlation force between YKL-40 and CTX-II (R=0.4 (p<0.05)) the connection was higher. Conclusion. Early stages of primary osteoarthritis are accompanied by degenerative changes inhyaline cartilages that is verified by T2*-relaxometry results, and increased excretion of Urine CTX-II on  the background of pro-inflammatory mediators hyperproduction, more significant increase in the concentration of cartilage glycoprotein YKL-40 compared to interleukin-1 (3 which comes together with the alteration of the system of correlations between the levels of pro-inflammatory cytokines and the parameters of degenerative dystrophic changes in articular cartilage.

 

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The estimation of biocompatibility of polycaprolactone matrices mineralized by vat-erite in subcutaneous implantation tests in white rats

Year: 2018, volume 14 Issue: №3 Pages: 451-456
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Kozadaev M.N., Tyapkina D.A., Kustodov S.V., Saveleva M.S., Bugaeva I.O., Parakhonsky B.V., Galashina E.A., Gladkova E.V., Norkin I.A.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

Aim: to estimate biocompatibility of matrices produced from polycaprolactone (PCL) and mineralized by vaterite (CaC03) by studying local and systemic manifestations of inflammatory reaction in subcutaneous implantation tests in white rats. Material and Methods. The experiment was conducted on 40 rats divided into four equal groups: control, comparison (rats with imitation of implantation), negative control (rats with implanted non-biocompatible matrices) and experimental group, comprised of animals with implanted PCL/CaC03-matrices. Local inflammatory manifestations were analyzed by morphological assay of implantation area tissues. Systemic inflammatory manifestations were estimated by TNF-a concentration and interleukin-lp (IL-1) in blood serum by ELISA. Results. The changes in cellular population content demonstrate that a PCL/CaC03-matriceonthe21 day after the implantation to rats is evenly colonizing by fibroblast cells and vascularizing. This type of matrices does not provoke intense inflammatory reaction seen in negative control animals and accompanied by systemic manifestations such as statistically significant rise in TNF-a and IL-1 concentrations. Conclusion. The data obtained in the study proving the biocompatibility of PLC/CaC03-scaffolds experimentally substantiate the potential for their use in tissue engineering.

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The study on the refraction of morphofunctional changes in kidneys of white male rats with experimental light desynchronosis

Year: 2018, volume 14 Issue: №3 Pages: 363-367
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Antonova V.M., Milashevskaya T.V., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

The goal is to study the morphofunctional changes in the kidneys under the influence of light desynchronosis, an analysis of the degree of reversibility of these changes. Material and Methods. The study was carried out in two stages on 72 white male rats. In the first series of experiments, animals underwent round-the-clock light exposure for 10 and 21 days. In the second series after round-the-clock exposure to light for 10 and 21 days, the beings were exposed to natural light for 21 days. The animals of the control group were kept in a natural light regime. Morphological and morphometric analysis of histological sections of kidneys stained with hematoxylin and eosin was performed. Results. It was found that on the 10th day of the experiment, cellular infiltration of the cortex occurs, and the edema of the interstitial tissue develops. These changes progress to the 21st day of the experiment and are combined with a significant decrease in the area of the glomerular capillary network and expansion of the lumen of the capsule of the glomerulus of cortical nephrons. When assessing the long-term consequences after 10-day illumination, the diameter of the renal corpus is reduced along the long and short axis. The changes registered on the 21st day do not reach the control values, which indicate that they are irreversible. Conclusion. The revealed structural and functional changes in the kidney indicate a negative effect of light desynchronosis: 10-day lighting leads to a breakdown of compensatory possibilities and the development of distant morphological changes; long-term light exposure (within 21 days) leads to the development of pronounced irreversible structural disorders.

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Peculiarities of microcirculatory reactions after subcutaneous implantation of polycaprolactone matrices mineralized by vaterite

Year: 2018, volume 14 Issue: №1 Pages: 35-41
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Norkin I.A., Ivanov A.N., Kurtukova M.O., Savelyeva M.S., Martyukova A.V., Gorin D.A., Parakhonsky B.V.
Organization: Saratov State Medical University, Saratov state university, Skolkovo Institute of Science and Technology
Summary:

The aim is to assess skin microcirculation changes arising during subcutaneous implantation of polycaprolactone scaffolds mineralized by vaterite. Material and Methods. The experiment was carried out on 30 albino rats divided into two groups: a negative control group and an experimental one. We implanted polycaprolactone scaffold with exhausted foreign protein subcutaneously into rats of the negative control group. We implanted polycaprolactone matrix mineralized by vaterite subcutaneously into the animals of the experimental group. Research methods include laser Doppler flowmetry and morphological examination of the tissues of the matrix implantation area. Results. Changes of skin microcirculation over the matrix allocation area correspond to the morphological pattern of tissue reactions. Biocompat-ibility disorders take the form of inflammation in the scaffold implantation area that is accompanied by stable perfusion rise associated with local bloodstream modulation changes. We did not observe inflammation signs in the surrounding scaffold tissues during implantation of polycaprolactone matrices mineralized by vaterite. Besides local microcircula-tory reactions possess a transient character disappearing completely by the 21st day after the implantation. Conclusion. Complex of the given functional and morphological studies allow us to ascertain high-grade biocompatibility of polycaprolactone matrices mineralized by vaterite thus giving rise to prospect of their use for tissue regeneration stimulation.

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Complex stimulation of peripheral nerve regeneration after deferred neurorrhaphy

Year: 2017, volume 13 Issue: №3 Pages: 732-737
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Ivanov A.N., Korshunova G.A., Matveyeva O.V., Ninel V.G., Shutrov I.E., Shchanitsyn I.N., Andronova Т.А., Norkin I.A.
Organization: Saratov Regional Child Clinical Hospital, Saratov State Medical University
Summary:

The aim is to study the complex stimulation effect including skin autotransplantation and electrical stimulation of the sciatic nerve on microcircular, electrophysiological and morphological changes after deferred neurorrhaphy in rats. Material and methods. The experiment was performed in 50 albino rats divided into control, comparative and experimental groups. In the experimental group, on the background of deferred neurorrhaphy, skin autotransplantation and electrical stimulation of the sciatic nerve had been carried out. In the comparative group only deferred neurorrhaphy was performed. Research methods included laser doppler flowmetry, electroneuromyography and morphological analysis of the operated nerve. Results. Complex stimulation including skin autotransplantation and direct action of electrical pulses on the sciatic nerve after its deferred neurorrhaphy causes restoration of bloodstream in the operated limb, promotes intensification of restoration of nerve fibers. Conclusion. Intensification of sciatic nerve regeneration after deferred neurorrhaphy in rats under the influence of complex stimulation including full-thickness skin graft autotransplantation and direct action of electrical pulses substantiates experimentally appropriateness of clinical testing of the given method for treatment of patients with peripheral nerve injuries.

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