Saratov JOURNAL of Medical and Scientific Research


Analysis of the genes polymorphisms distribution of the MMP1, XRCC1, HFE (2), GSTT in patients with non-neoplastic melanin hyperpigmentation

Year: 2017, volume 13 Issue: №3 Pages: 590-594
Heading: Dermatovenerology Article type: Original article
Authors: Ikonnikova EV, Kruglova LS, Shatokhina EA, Talybova AM.
Organization: Central State Medical Academy of the ADP of the Russian Federation

Objective: to study the factors of increased risk of hyperpigmentation, with the polymorphism of MMP1, XRCC1, HFE (2), GSTT genes and analysis of their association in patients with various clinical forms of non-neoplastic melanin hyperpigmentation. Material and methods. The study included 38 patients with hyperpigmentation (melasma / chlo-asma, post-traumatic/ post-inflammatory hyperpigmentation, solar lentigo). All patients were underwent buccal swabs with sterile cotton buds to take the material for the study. All patients were underwent genetic analysis of polymorphism of HFE (2), XRCC1, MMP1, and GSTT genes by genotyping single-nucleotide substitutions. Results. In patients with melasma / chloasma, the high value of the alleles cipher of MMP1, XRCC1, and GSTT genes was noted, while the cipher of HFE (rs1799945) and HFE (rs1800562) allele index showed average values. In patients with post-traumatic / post-inflammatory pigmentation, the high value ofthe alleles cipher of MMP1, HFE (rs1799945) and HFE (rs1800562), GSTT genes was noted, while XRCC1 allele cipher showed average values. In patients with solar lentigo, the high value ofthe alleles cipher of MMP1, XRCC1, and GSTT genes was noted, while the cipher of HFE (rs1799945) and HFE (rs1800562) allele index showed average values. Conclusion. Was revealed the similarity ofthe results of genetic research in patients with melasma / chloasma and in patients with solar lentigo.

2017_03-1_590-594.pdf293.3 KB

Antiradiation properties of melanin

Year: 2014, volume 10 Issue: №4 Pages: 828-832
Heading: radiation medicine Article type: Original article
Authors: Bushmanov A.Ju., Ivanov А.А., Andrianova I.E., Stavrakova N.M., Bulinina T.M., Dorozhkina O.V.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency

Aim: to examine the therapeutic efficiency of melanin administered to mice after irradiation at lethal and sublethal doses. Material and methods: Survival and hematological states were studied on CD-1 mice receiving on acute or fractionated whole-body doses on X-rays or gamma-irradiation. Melanin soluble was given with water ad libitum from the first to the 30th-day after irradiation. Results. It was shown that melanin produced a significant therapeutic and protective-therapeutic action against acute radiation injury in the dose range 6,5-7,5 Gy (LD80-LD under our conditions). Cumulative survival melanin treated mice was increased to 14,4%, in control group — 1,9%. After fractionated injury (1 Gy daily, total dose 10 Gy) all mice which consumed melanin remained alive, versus 43,7% in control. Melanin decreased radiation-induced damage and stimulated the hematopoiesis recovery after sublethal exposure (5Gy). Conclusion. The results permit to regard melanin as a therapeutic agent for treatment of radiation injuries.

2014_04-01_828-832.pdf347.57 KB


Year: 2008, volume 4 Issue: №4 Pages: 53-55
Heading: Ophtalmology Article type: Original article
Authors: E. V. Koblova, T. G. Kamenskikh, A. N. Bashkatov, E. A. Genina, V. V. Tuchin
Organization: Saratov National Research University n.a. N. G. Chernyshevsky, Saratov State Medical University

Based on the experimental data obtained in vivo from digital analysis of color images of human irises, the mean melanin content in human eye irises has been estimated. For registration of color images the digital camera Olympus C-5060 has been used. The images have been obtained from irises of healthy volunteers as well as from irises of patients with open-angle glaucoma. The computer program has been developed for digital analysis of the images. The result has been useful for development of novel methods and optimization of already existing ones for non-invasive glaucoma diagnostics.

2008_04_053_055.pdf227.95 KB