Saratov JOURNAL of Medical and Scientific Research


The assessment of molecular markers of cell interaction and lipid peroxidation in rats with alloxan diabetes and trans- planted liver cancer after intravenous injection of gold nanorods

Year: 2015, volume 11 Issue: №2 Pages: 107-112
Heading: Biochemistry Article type: Original article
Authors: Bucharskaya А.В., Dikht N.I., Afanasyeva G.A., Terentyuk G.S., Zakharova N.B., Maslyakova G.N., Khlebtsov B.N., Khlebtsov N.G.
Organization: Institute of Biochemistry and Physiology of Plants and Microorganisms of Russian Academy of Sciences, Saratov State Medical University

Цель: оценить воздействие однократного внутривенного введения покрытых полиэтиленгликолем золотых наностержней на молекулярные маркеры межклеточного взаимодействия и показатели перекисного окисления в сыворотке крови четырех групп крыс: интактных, с аллоксановым диабетом, с перевитым раком печени; с перевитым раком печени на фоне аллоксанового диабета. Материалы и методы. Активность перекисного окисления липидов оценивалась по показателям гидроперекисей липидов, малонового диальдегида и молекул средней массы с использованием общепринятых спектрофотометрических методов, исследования молекулярных маркеров — фактора некроза опухоли TNF-a, инсулиноподобного фактора роста IGF-1, васкулоэн-дотелиального фактора роста VEGF-C проводились в сыворотке крови методом иммуноферментного анализа. Результаты. Выявлено, что при однократном внутривенном введении золотых наностержней не происходит значительного изменения количества маркеров межклеточного взаимодействия и показателей перекисного окисления липидов. в периферической крови. Заключение. Полученные результаты позволяют рекомендовать использование покрытых полиэтиленгликолем золотых наностержней в экспериментальной онкологии.

2015_02_107-112.pdf407.38 KB

Molecular-genetic assessment of angiotensin-converting enzyme hyperactivity determinancy risk at hemorrhagic fever with renal syndrome

Year: 2010, volume 6 Issue: №1 Pages: 014-017
Heading: Biochemistry Article type: Original article
Authors: A.A. Baygildina, D.V. Islamgulov, F.Kh. Kamilov, T.A. Khabelova, I.R. Minniakhmetov
Organization: Ufa scientific centre of the RAS, Bashkir State Medical University of the Russian Health Care

The research aimed to explore the changes of angiotensin-converting enzyme (ACE) blood activity and polymorphism of the angiotensin II type 1 receptor gene as disease predictor at hemorrhagic fever with renal syndrome (HFRS). In exami¬nation 409 patients at the age of 15-65 years were involved. ACE blood activity with the help of Bbhlmann (Switzerland) ACE kinetic test was determined. Blood genomic DNA by phenol- chloroformic extraction was isolated, genetic locuses by polymerase chain reaction of DNA synthesis were researched. It was shown that the ACE blood activity at HFRS is risen and these changes the significanter the severer form of disease. Nonsignificant rising of enzyme activity only in feveric period of both ungravic and gravic forms was observed. In gravic form significant changes of ACE activity are observed, in complicated form - stable high enzyme activity during all disease took place. Analysis of angiotensin II type 1 receptor gene polymorphism shown that alleles *A1166 and *C1166, genotypes *A1166/*A1166 and *C1166/*C1166 are not associated with HFRS severity. Made conclusion that high ACE activity is not adaptive reaction due to defect in angiotensin II binding and it is an adequate metabolic response of an organism to endotheliotropic virus action

201001_014_017.pdf408.93 KB

Comparative study of antibacterial action of iron and copper nanoparticles on clinical Staphylococcus aureus strains

Year: 2010, volume 6 Issue: №1 Pages: 011-014
Heading: Biochemistry Article type: Original article
Authors: I.V. Babushkina, V.B. Borodulin, G.V. Korshunov, D.M. Puchinjan
Organization: Saratov State Medical University, Saratov Scientific Research Institute of Traumatology and Orthopedics

Research objective is to study antibacterial action of nanoparticles of iron and copper on polyantibiotically resistant clinical Staphylococcus aureus strains.
Materials and methods include antibacterial action of nanoparticles of copper and iron on 10 Staphylococcus au¬reus strains, isolated from patients with purulent complications stayed in the in-patient department of traumatology and orthopedics. Solutions of powders of iron and copper have been prepared directly before the experiment in concentra¬tion from 0,001 to 1 mg/ml.
it has been revealed that the influence of nanoparticles on growth of clinical strains and the intensity of antibacterial effect depends on the form of nanoparticles, their concentration and action time. concentration of 0,1 mg/ml and 1 mg/ ml of iron nanoparticles has provoked the decrease in quantity of microbe cells from 3 to 34 % (p <0,01). in smaller concentrations the reliable antibacterial effect has not been observed. Antibacterial activity of copper nanoparticles has been expressed in a wide range of concentrations from 0,001 mg/ml to 1 mg/ml, even during short-term action (30 minutes) it has provoked reduction of quantity of the microbe cells grown on the firm nutrient medium, 97-100 % in comparison with the control (p <0,001).
in conclusion it is to point out that copper nanoparticles have more expressed inhibitory effect on growth of clini¬cal strains of golden staphylococcus than iron nanoparticle suspension. inhibition degree depends on superdispersed powder dosage and incubation period

201001_011_014.pdf357.61 KB

Role Of Adhesion Molecules Vcam-1 And Ve-Cadherin In Endothelium Dysfunction Development At Hemorrhagic Fever With Renal Syndrome

Year: 2009, volume 5 Issue: №4 Pages: 478-481
Heading: Biochemistry Article type: Original article
Authors: А.А. Baygildina
Organization: Bashkir State Medical University of the Russian Health Care

The research goal is to determine the changes in concentration of both sVCAM-1 and VE-cadherin in blood serum of patients suffered from hemorrhagic fever with renal syndrome (HFRS). 87 patients aged 15-65 were examined. Concentrations of both sVCAM-1 and VE- cadherin in blood serum by means of "Bender MedSystems" (Austria) ELISA test were determined. It was shown that in both medium severe and severe forms of HFRS statistically the significant rise of sVCAM-1 concentration in blood with high indices in oliguric period took place. Complicated form was characterized by high indices of sVCAM-1 level in fever period, extremely decreasing in concentration in oliguric period and tendency to normalizing in clinical convalescence period. VE-cadherin level in blood was predominantly lower than control in all the observed groups with the exception of fever period in group with medium severe disease form. Negative correlation of normal intensity between adhesion molecules levels in blood was revealed. In conclusion it is necessary to point out that high VCAM-1 expression by endotheliocytes evidences the development of an adhesion form of endothelial dysfunction, low VE-cadherin production in a base for development of angiogenic form of endothelial dysfunction and changes in expression of these adhesion molecules that have adaptive metabolic response to macroorganism of HFRS pathogenic action

200904_478_481.pdf351.14 KB

Interaction of Big Celandine Extract and Plasmid DNA, Excreted From the Clinical Strain Escherichia Coli

Year: 2009, volume 5 Issue: №3 Pages: 310-312
Heading: Biochemistry Article type: Original article
Authors: I.V. Babushkina., A.A. Svistunov, H.-M.P. Chomaev, E.G. Chebotarjova, N.A. Belskaya
Organization: Saratov Scientific Research Institute of Traumatology and Orthopedics, Saratov State Medical University

Search for compounds which possess antibacterial activity and have an effect on plasmid DNA, is one more of the important problems of applied medicine. Natural substances are the most desirable candidates for this role.
Plasmid DNA excretion was carried out from the strain Echerihiacoli, derived from patient N., who suffered from chronic pyelonephritis, in the bacteriological laboratory of the Regional clinical hospital of Saratov according to the modified method Birnboim and Doli. The calculation of alkaloid concentration was made, e.g. Sanguinarin and Cheler-etrin contained in celandine extract.
The devised method has been tested on five celandine tincture samples, stored for the research of the stability of the content of alkaloids and other quality factors of the given preparation. In so doing the content of the alkaloid sum in tincture changes within the limits of 1 %. Abatement of plasmid DNA concentration after the cell treatment by celandine extract is revealed.

200903_310_312.pdf432.72 KB