Saratov JOURNAL of Medical and Scientific Research

Lagutina D.D.

Saratov State Medical University n. a. V. I. Razumovsky, Assistant of Central Scientifc Research Laboratory

The role of histaminergic system in the effects of skin flap autotransplantation on microcirculation at absolute insulin deficiency

Year: 2020, volume 16 Issue: №2 Pages: 521-525
Heading: Рathophysiology Article type: Original article
Authors: Antipova O.N., Lagutina D.D., Stepanova T.V., Savkina A.A., Kuznetsova N.A., Andronova Т.А., Kiriyazi T.S, Ivanov A.N.
Organization: Saratov Medical University "Reaviz", Saratov State Medical University
Summary:

The objective of the article is to estimate the impact of the histaminergic system in the effects of skin flap autotrans-plantation (SFA) on microcirculation in white rats with alloxan insulin deficiency. Material and Methods: The studies were carried out in 60 white rats, divided into 3 groups: 20 control intact animals, 20 animals with alloxan diabetes — the comparison group, 20 animals with alloxan diabetes and SFA— the experimental group. The mechanisms of blood circulation modulation and perfusion of posterior paw dorsal skin, the concentration of histamine in the blood serum, and morphological changes in the SFAarea were evaluated. Results: The significant increase of skin perfusion on 43%, redistribution of microcirculation modulation active mechanisms and the increase in histamine serum concentration in 2,5 times underthe influence of SFA were observed in rats of the experimental group. Conclusion: The distant stimulating effect of SFA on microcirculation in conditions of absolute insulin deficiency is realized with increasing histamine serum concentration. Moreover, the stimulation of the blood flow regulation active mechanisms by increasing the histamine concentration is not equivalent — the effects on endothelium-dependent and neurogenic regulation mechanisms prevail, and the activation of histamine smooth muscle cell receptors is much less pronounced.

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The relation of carbohydrate metabolism disorders and markers of endothelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap

Year: 2019, volume 15 Issue: №2 Pages: 379-38
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Popykhova E.B., Ivanov A.N., Stepanova T.V., Pronina E.A., Lagutina D.D.
Organization: Saratov State Medical University
Summary:

The purpose of this work is to study the relationship of carbohydrate metabolism disorders and markers of endo-thelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap. Material and Methods. The study was conducted on 60 male white outbred rats, which were divided into: an intact control group, a two comparative and an experimental group. In animals of the comparison group, alloxan diabetes mellitus (DM) was caused, in rats from the experimental group, an autotransplantation of the skin fap was performed against the background of developed alloxan diabetes. The levels of glycated hemoglobin (HbA1c), glucose, C-reactive protein (CRP) and vascular endothelial growth factor (VEGF) were determined. Results. Autotransplantation of the skin fap causes a normalization of the glycemic profle in animals of the experimental group, and also reduces subclinical infammation caused by hyperglycemia and stabilizes the expression of VEGF.Diabetes causes an increase in HbA1c by 69 % and CRP by 11 %, while аutotransplantation of the skin fap leads to a decrease in the concentration of HbA1c by 28 % and CRP by 7 %. Conclusion. Autotransplantation of the skin fap reduces the risk of microcirculatory disorders due to hyperglycemia and chronic subclinical infammation.

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