Saratov JOURNAL of Medical and Scientific Research

ZIobina O.V.

Saratov State Medical University n.a. V. I. Razumovsky Department of Histology, Cytology, Embryology, Assistant Professor, Candidate of Medical Sciences

Intense light deprivation as a factor in stress disorders of behavioral reactions and cognitive functions in the experiment

Year: 2019, volume 15 Issue: №2 Pages: 363-368
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Antipova O.N., Ivanov A.N., Zlobina O.V., Kirichuk V.F., Surovtseva K.A., Ankina V.D., Bondar G.D., Zenkina T.M., Polyukova M.V.
Organization: Saratov State Medical University
Summary:

Objective: to study the efect of light desynchronosis on behavioral reactions and cognitive functions of white male rats. Material and Methods. The study was conducted on 36 intact non-pedigree white male rats, which were divided into 2 groups: experimental and control. Animals were exposed to artifcial illumination 24 hours a day provided by the fuorescent lamp of 60 kW capacity. The same animals of the experimental group on the 10th, 21st and 30th days were tested in the installations «Dark-light camera» and «Open feld». Results. On the 10th day of intensive light deprivation, the indicators refecting the research-orientation activity reached the maximum values, which indicated the development of stress damage to the animal organism. On the 21st day of the experiment, the

Keywords: pneumocysts
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Morphofunctional state of spleening under light desynchronosis in the experiment

Year: 2018, volume 14 Issue: №3 Pages: 533-536
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Karetnikova A.Yu., Terekhina E.S., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

 Aim: to study the effects of light desynchronosis on the morphofunctional state of the spleen of white male rats. Material and Methods. The study of light desynchronosis was carried out on 48 male rats using the Light Light model with an illumination power of 300 Ix in the daylight and 500 Ix in the dark. The laboratory animals in the experiment were divided into four groups: the control and three experimental ones, in which they were exposed to light for 1 day, 10 days, 21 days respectively. In the course of morphometry of histological sections of the spleen, the following parameters were assessed: the area of white pulp lymphoid follicles, B- and T-clependent zones of white pulp, the number of germinative centers and immunoblasts in B- and T-zones. Results. On day 1 of the experiment, an increase in the area of the lymphoid follicle, periarterial and B-dependent zones, an increase in the number of immunoblasts and reproduction centers was registered. By the 10th day there was a partial restoration of these indicators relative to the control values. On the 21st day of the experiment, a decrease in the growth dynamics of all the morphometric parameters of the spleen was observed. Conclusion. Light desynchronosis induces by the Light Light model leads to the development of the pathology of the immune system with an extensive shift of all measurable indicators. Key words: morphofunctional state of the spleen, morphometric criteria, light desynchronosis, immunity.

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The study on the refraction of morphofunctional changes in kidneys of white male rats with experimental light desynchronosis

Year: 2018, volume 14 Issue: №3 Pages: 363-367
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Antonova V.M., Milashevskaya T.V., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

The goal is to study the morphofunctional changes in the kidneys under the influence of light desynchronosis, an analysis of the degree of reversibility of these changes. Material and Methods. The study was carried out in two stages on 72 white male rats. In the first series of experiments, animals underwent round-the-clock light exposure for 10 and 21 days. In the second series after round-the-clock exposure to light for 10 and 21 days, the beings were exposed to natural light for 21 days. The animals of the control group were kept in a natural light regime. Morphological and morphometric analysis of histological sections of kidneys stained with hematoxylin and eosin was performed. Results. It was found that on the 10th day of the experiment, cellular infiltration of the cortex occurs, and the edema of the interstitial tissue develops. These changes progress to the 21st day of the experiment and are combined with a significant decrease in the area of the glomerular capillary network and expansion of the lumen of the capsule of the glomerulus of cortical nephrons. When assessing the long-term consequences after 10-day illumination, the diameter of the renal corpus is reduced along the long and short axis. The changes registered on the 21st day do not reach the control values, which indicate that they are irreversible. Conclusion. The revealed structural and functional changes in the kidney indicate a negative effect of light desynchronosis: 10-day lighting leads to a breakdown of compensatory possibilities and the development of distant morphological changes; long-term light exposure (within 21 days) leads to the development of pronounced irreversible structural disorders.

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Morphological changes in liver of laboratory animals at prolonged intake of gold nanoparticles

Year: 2014, volume 10 Issue: №2 Pages: 250-256
Heading: Macro- and Micromorphology Article type: Original article
Authors: Pakhomy S.S., Bucharskaya А.В., Navolokin N.A., Maslyakova G.N., Zlobina O.V., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University
Summary:

Aim: to evaluate the severity of morphological changes in the liver of laboratory animals with prolonged intake of pegilated gold nanoparticles of different sizes from 2 to 50 nm. Material and methods. The experiment was performed on 240 albino rats divided into 4 groups. The animals of experimental groups were orally administered the gold nanoparticles in a specific pattern. Results. Morphological changes in liver depend on the particle size and the duration of administration. Developing pathological changes in the liver are reversible, as evidenced by the gradual recovery of the liver structure 14 days after administration. Conclusion. The most significant changes in the liver were found in the experimental groups with a 30-day administration of 2 and 50 nm gold nanoparticles that show the size-dependent nature of their impact.

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Morphological changes in the organs of laboratory animals during the prolonged oral administration of gold nanoparticles

Year: 2013, volume 9 Issue: №2 Pages: 208-213
Heading: Macro- and Micromorphology Article type: Original article
Authors: Maslyakova G.N., Pakhomiy S. S., Bucharskaya А.В., Zlobina O.V., Navolokin N.A., Ponukalin A.N., Khlebtsov N.B., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University, Институт биохимии и физиологии растений и микроорганизмов РАН
Summary:

The purpose of the article is to assess the severity of the morphological changes in the internal organs of laboratory animals with prolonged oral administration of gold nanoparticles of different sizes. Material and methods: The experiment was carried out on 40 albino rats which were divided into 4 groups. Animals of the experimental group received oral gold nanoparticles by the appropriate scheme. Results: At comparing the morphological changes in the internal organs of laboratory animals it was established that the introduction of gold nanoparticles of different sizes leads to the same type of disturbances of morphological structure, the gold nanoparticles of 1-3 and 50 nm cause the most expressive changes. Conclusion: The most significant morphological modifications in the internal organs were caused by gold nanoparticles of 1-3 and 50 nm. It points out to the size-dependence of the effect of gold nanoparticles.

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Analysis of accumulation and biodistribution of gold nanoparticles in mesenteric lymph nodes by oral administration

Year: 2013, volume 9 Issue: №1 Pages: 17-20
Heading: Macro- and Micromorphology Article type: Original article
Authors: Zlobina O.V., Pakhomiy S. S., Bucharskaya A.B., Bugaeva I.O., Maslyakova G.N., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University, Институт биохимии и физиологии растений и микроорганизмов РАН
Summary:

The aim of the article is to conduct analysis of accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats. Particular attention is paid to the size and duration of oral administration. Material and Methods: Dark field microscopy with Leica DM 2500 microscope has been used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher has been used to confirm gold nanoparticles identification. Results: It has been found that regardless of the duration of administration gold nanoparticles of 1-3 nm have not been detected by these methods in the lymph nodes. Gold nanoparticles of 15 and 50 nm have been found out in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It has been established that the gold nanoparticles of 15 and 50 nm has been detected in the form of clusters in the cytoplasm of macrophages and lymphocytes.

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Morphokinetics of mesenterial lymphatic node cell populations in exposure of gold nanoparticles within experimental work

Year: 2011, volume 7 Issue: №2 Pages: 353-357
Heading: Macro- and Micromorphology Article type: Original article
Authors: Zlobina O.V., Bugaeva I. О., Maslyakova G.N., Firsova S.S., Bucharskaya А.В., Khlebtsov N.G., Khlebtsov B.N., Dykman L.A.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky, Saratov State Medical University
Summary:

The research goal is to investigate the influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals. Experiment included 24 white rats. The investigation was conducted in 4 groups of animals. The animals were administered the gold nanoparticles orally for 15 days. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological alterations in the mesenterial lymphatic nodes assisted in activation of migration processes, the proliferation and differentiation. The immu-nomodulatory action of gold nanoparticles was proved

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Kinetiks of cellular populations under influence of infra-red low-power laser irradiation

Year: 2010, volume 6 Issue: №1 Pages: 023-026
Heading: Macro- and Micromorphology Article type: Original article
Authors: I.O. Bugaeva, A.V. Egorova, O.V. Zlobina
Organization: Saratov State Medical University
Summary:

The morphokinetiks of cellular populations under the influence of infra-red low-power laser irradiation has been studied. our researchers evidenced, that the number of cortical thymocytes decreases under the influence of infra-red low-power laser irradiation. Invasion of Mast cells in to the stroma of thymus and their contact with the reticuloepithelio-cytes are the important factors for the stimulation of proliferation and differentiation of thymocytes

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HISTOFUNCTION TRANSFORMATION IN ENDOCRINE AND IMMUNE ORGANS UNDER VARIOUS REGIMEN INFLUENCE OF ELECTROMAGNETIC RADIATION

Year: 2009, volume 5 Issue: №1 Pages: 36-40
Heading: Macro- and Micromorphology Article type: Original article
Authors: E.B. Rodzaevskaya, J.V. Polina, I.A. Uvarova, V.D. Tupikin, L.I. Naumova, N.V. Bogomolova, M.O. Kurtukova, O.V. ZIobina
Organization: Saratov State Medical University, Astrakhan State Medical University
Summary:

The effects of resonance and no-resonance of the low-intensify EMW frequency regimes were examined in Vistar rats,males. The microscopic structures and histochemical changes in thyroid, thymus,adrenals,ovaries,and other organs were studied. Our researchers evidenced, that the resonance EMW frequency regime slightly stimulates cell mitotic activity and exchange of substances in organ’s parenchyma and micro-circulation in connective tissue stroma. Treatment the course of no-resonance frequency EMW regime deteriorates the ratio between parenchyma and mesenchyme components correlation is detected in organ.

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