Saratov JOURNAL of Medical and Scientific Research

Tyapkina D.A.

Saratov State Medical University n.a. V I. Razumovsky, Department of General Medicine, Student

Comparative characteristics of microcirculatory changes in white rats during subcutaneous implantation tests of polycaprolactone scaffolds containing vaterite and hydroxyapatite

Year: 2019, volume 15 Issue: №1 Pages: 98-103
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Chibrikova Yu.A., Kustodov S.V., Tyapkina D.A., Bugaeva I.O., Norkin IA.
Organization: Saratov State Medical University
Summary:

Objective: comparative evaluation of skin microcirculatory functional changes over the implantation area of polycap-rolactone scaffolds with mineral carriers: vaterite and hydroxyapatite. Material and Methods. The experimental study was performed on 43 rats divided into three groups: two test groups and one negative control group. Polycaprolactone PCL-scaffolds containing ovalbumine and of no biocompatibility were implanted into the rats of negative control group (n=15). The implantation of PCL- scaffolds containing vaterite under their skin was performed on rats of the first test group, and animals of the second test group were implanted with PCL-scaffolds containing hydroxyapatite. The method of laser Doppler flowmetry was used to study the microcirculatory flow. Results. The constant increase in the perfusion index has been observed in the area of non-biocompatibile scaffolds implantation. Thechanges in local skin blood flow modulation have been also detected. The transient character of local microcirculatory responses with total normalization of skin perfusion and its mechanisms has been noticed by the 21st day of the trial following PCL-scaffolds with vaterite and hydroxyapatite implantation. Conclusion. The dynamic monitoring has allowed finding that mineralization of PCL-scaffolds with vaterite as well as hydroxyapatite leads to no prominent changes in skin perfusion at subcutaneous implantation tests on white rats suggesting the biocompatibility of these scaffold types.

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The estimation of biocompatibility of polycaprolactone matrices mineralized by vat-erite in subcutaneous implantation tests in white rats

Year: 2018, volume 14 Issue: №3 Pages: 451-456
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Kozadaev M.N., Tyapkina D.A., Kustodov S.V., Saveleva M.S., Bugaeva I.O., Parakhonsky B.V., Galashina E.A., Gladkova E.V., Norkin I.A.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

Aim: to estimate biocompatibility of matrices produced from polycaprolactone (PCL) and mineralized by vaterite (CaC03) by studying local and systemic manifestations of inflammatory reaction in subcutaneous implantation tests in white rats. Material and Methods. The experiment was conducted on 40 rats divided into four equal groups: control, comparison (rats with imitation of implantation), negative control (rats with implanted non-biocompatible matrices) and experimental group, comprised of animals with implanted PCL/CaC03-matrices. Local inflammatory manifestations were analyzed by morphological assay of implantation area tissues. Systemic inflammatory manifestations were estimated by TNF-a concentration and interleukin-lp (IL-1) in blood serum by ELISA. Results. The changes in cellular population content demonstrate that a PCL/CaC03-matriceonthe21 day after the implantation to rats is evenly colonizing by fibroblast cells and vascularizing. This type of matrices does not provoke intense inflammatory reaction seen in negative control animals and accompanied by systemic manifestations such as statistically significant rise in TNF-a and IL-1 concentrations. Conclusion. The data obtained in the study proving the biocompatibility of PLC/CaC03-scaffolds experimentally substantiate the potential for their use in tissue engineering.

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