Saratov JOURNAL of Medical and Scientific Research

Kolmakova T.S.

Rostov State Medical University, Head of the Department of Medical Biology and Genetics, Docent, Doctor of Biological Sciences

Possible laboratory and instrumental markers of Parkinson's disease

Year: 2020, volume 16 Issue: №1 Pages: 336-341
Heading: Neurology Article type: Original article
Authors: Goncharova Z.A., Kolmakova T.S., Oksenyuk O.S., Morgul E.V., Gelpey M.A., Vlasova N.D., Smirnova O.B., Mutalieva Kh.M.
Organization:
Summary:

Objective: to evaluate the role of a complex of markers of inflammatory processes and free radical oxidation, an immunoreactivity profile, stabilographic indicators in the pathogenesis of Parkinson's disease (PD) for possible use as clinically significant markers of early diagnosis of the disease. Material and Methods. 139 patients with PD, 31 patients as the control, and 11 patients as the comparison with Parkinson's syndrome were examined. The following parameters were evaluated in blood serum: antidodies to NF-200, GFAP, S100, MBP, voltage-dependent-Ca-chan-nel, Na-Choline, glutamate, dopamine, serotonin, Mu-Opiate, GABA receptors, (3-Endorphin; total antioxidant activity; malondialdehyde's accumulation; determination of small, medium, large and giant circulating immune complexes and medium-weight molecules. Postural functions were evaluated using a biofeedback computer stabilizer. Results. Significant differences in the profiles of immunoreactivity, neuroinflammation and endogenous intoxication in patients and in the control group were revealed. The stabilometric indicator of the quality of the equilibrium function significantly varied depending on the stage of PD. Conclusion. The results indicate a possible role of activation of the immune system and neuroinflammation in the process of neurodegeneration in PD. The autoimmune response is specific in nature, progressively changing depending on the stage of the disease.

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Distinguishing features of the range of serum antibodies to nervous tissue antigens in Parkinson's disease

Year: 2019, volume 15 Issue: №1 Pages: 151-154
Heading: Neurology Article type: Original article
Authors: Goncharova Z.A., Kolmakova T.S., Oksenjuk O.S., Morgul E.V., Gelpey M.A., Vlasova N.D., Kalmykova Yu.A.
Organization:
Summary:

The goal of this study was to perform a multivariable analysis of immunobiochemical parameters which reflect the state of nervous system microstructure in Parkinson's disease (PD). Materials and Methods. The study included 54 patients of the main group with a diagnosis of PD and 6 patients of the control group. The immunoreactivity profiles of natural regulatory auto-AB in serum were determined by ELISA using the ELI-P-Complex assay. The following parameters were assessed: ABto NF-200, GFAP, S100 proteins, MBP, beta-endorphin, voltage dependent Ca2+channel, H-cholinergic receptors, glutamate, dopamine, serotonin, opiate, GABA receptors. Results. The study revealed significant abnormalities in auto-AB profiles of the examined patients. Conclusion. The findings on the difference in range of auto- AB dependent on the duration of the disease and the revealed associations with the main characteristics of the disease can be used both for early diagnosis and for predicting the course of PD in the future.

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Investigation of the oxidative status in patients with Parkinson's disease

Year: 2018, volume 14 Issue: №1 Pages: 132-135
Heading: Neurology Article type: Original article
Authors: Goncharova Z.A., Kolmakova T.S., Okseniuk O.S., Gelpey M.A., Isachkina N.S.
Organization:
Summary:

Currently, much attention is paid to the search for laboratory diagnostic tests for Parkinson's disease, which can be used to create a screening diagnostic panel. The aim of the research was to study markers of oxidative status in blood plasma and to correlate these indicators with the main clinical characteristics of Parkinson's disease. Material and Methods. The study included 32 patients of the main group and 21 patients as a control group. Blood was collected for the evaluation of total oxidative activity and general antioxidant status of the organism (OOAand OAA): determination of the activity of catalase, superoxide dismutase (SOD), malonic dialdehyde (MDA) in the blood serum by spectropho- tometric method. Results. The parameters of SOD, catalase, ОАА, OOA in patients with early stage of Parkinson's disease significantly differed from those of the control group Conclusion. Reduction of oxidative activity could slow down the development of the disease.

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