Saratov JOURNAL of Medical and Scientific Research

Rastorgueva A.A.

State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, the Center of Biomedicine Technologies, Laboratory Technician

Application of cell technologies in thermal burn damage to skin (Practical experience in State Research Center— Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency of Russia)

Year: 2019, volume 15 Issue: №4 Pages: 999-1004
Heading: Тhematic supplement Article type: Original article
Authors: Samoilov A.S., Astrelina Т.А., Aksenenko A.V., Kobzeva I.V., Suchkova Yu.B., Nikitina V.A., Usupzhanova D.Yu., Brunchukov V.A., Brumberg V.A., Rastorgueva A.A., Makhova A.E., Karaseva T.V., Lomonosova Е.Е., Dobrovolskaya E.l., Udalov Yu.D.
Organization: Moscow Helmholtz Research Institute of Eye Diseases, State Research Center— Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to present the results of the use of autologous regenerative cells of adipose tissue (AT) in treatment of patients with deep thermal burns. Material and Methods. The use of cellular technologies was carried out on 5 patients (4 men and 1 woman) aged 23-45 years, with deep thermal burns of III—IV degree of various etiology and localization, lesions from 1 % to 80%. Patients received conventional conservative therapy for burn wounds. After stabilization of the patient's general condition and positive dynamics of the local process in the wound during the planned surgical treatment of burn surfaces, the biological material of the AT was obtained. The volume of lipoaspirate AT was 195.0±74.2 ml. For clinical use of AT, the stromal-vascular fraction (SVF) of AT was obtained. The patients received intradermal injection of autologous SVF in the amount of 10 million to 50 million cells in a volume of 5 ml in 10-15 points around the damage to skin. Results. Upon examination (2.5 months after the point of introduction of SVF of AT), patients showed good cosmetic and functional results. The skin is soft, elastic, there are no rough hypertrophic and keloid scars, no contractures. Scars do not stand out. Movement in the joints is saved. Conclusion. The use of SVF AT in the early periods after injury contributed to activation of reparative processes in the dermis, which reduced the local inflammatory response, accelerated epithelialization, restoring skin elasticity with a decrease in the severity of fibrous scars and resulted in the lack of mobility in the joints.

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Elaborating of a cryoconservation protocol of blood vessels for the tissue-specific matrices

Year: 2017, volume 13 Issue: №4 Pages: 891-900
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Rastorgueva A.A., Lomonosova Е.Е., Kobzeva I.V., Makhova A.E., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to create a protocol for complex cryoconservation of blood vessels in polydimethylsiloxane to create tissue-specific matrices. Material and Methods. The material ofthe study was the iliac arteries from postmortem donors, seized in the multi-organ donation, rejected for unclaimed and disposed of later. Polydimethylsiloxane was used as a coolant (PDMS) with a viscosity of 5-25 centistokes (Spectroplast, Russia). Bench testing of cooling and heating ofthe blood vessel in PDMS, testing of heating and cooling of PDMS up to operating temperatures (-80°C) in different ways and variants of its thermal insulation, assessment of different cooling rates ofthe vessel during cryoconservation, modeling of 3 types of working chambers for cryoconservation, sterilization and defrosting of up to 10 vessels, modeling of cryoconservation and thawing of vessels in PDMS under different conditions, selection of substrates from silicone and plastic for uniform fixation of vessels which are of size or size in the working chambers or without them. Results. The developed protocol allows cryopreserved from 1 to 10 or more vessels of all basic sizes both immediately after removal and after transportation to the laboratory, to vary the cooling rate from 1.5 to 300°C/min and above, to be used with different levels of laboratory equipment, as with liquid nitrogen, dry ice, and only with a medical freezer. Conclusion. The developed protocol allows to apply it in cases of complex cryoconservation of blood vessels to create tissue-specific matrices and can be recommended for preclinical testing.

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