Saratov JOURNAL of Medical and Scientific Research

Pronina E.A.

Saratov State Medical University n.a. V. I. Razumovsky Senior Research Assistant of Central Scientific Research Laboratory; Yuri Gagarin State Technical University of Saratov, Professor of Department of Physical Materials Science and Biomedical Engineering, Associate Professor, DSc

The relation of carbohydrate metabolism disorders and markers of endothelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap

Year: 2019, volume 15 Issue: №2 Pages: 379-38
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Popykhova E.B., Ivanov A.N., Stepanova T.V., Pronina E.A., Lagutina D.D.
Organization: Saratov State Medical University
Summary:

The purpose of this work is to study the relationship of carbohydrate metabolism disorders and markers of endo-thelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap. Material and Methods. The study was conducted on 60 male white outbred rats, which were divided into: an intact control group, a two comparative and an experimental group. In animals of the comparison group, alloxan diabetes mellitus (DM) was caused, in rats from the experimental group, an autotransplantation of the skin fap was performed against the background of developed alloxan diabetes. The levels of glycated hemoglobin (HbA1c), glucose, C-reactive protein (CRP) and vascular endothelial growth factor (VEGF) were determined. Results. Autotransplantation of the skin fap causes a normalization of the glycemic profle in animals of the experimental group, and also reduces subclinical infammation caused by hyperglycemia and stabilizes the expression of VEGF.Diabetes causes an increase in HbA1c by 69 % and CRP by 11 %, while аutotransplantation of the skin fap leads to a decrease in the concentration of HbA1c by 28 % and CRP by 7 %. Conclusion. Autotransplantation of the skin fap reduces the risk of microcirculatory disorders due to hyperglycemia and chronic subclinical infammation.

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Features of angiogenesis in skin tissue regeneration

Year: 2019, volume 15 Issue: №1 Pages: 104-107
Heading: Physiology and Pathophysiology Article type: Review
Authors: Pronina Е.А., Stepanova T.V., Kiriyazi T.S., Maslyakov V.V., Ivanov A.N.
Organization: Saratov Medical University "Reaviz", Saratov State Medical University, Saratov State Technical University n.a. Yu.A. Gagarin
Summary:

Restoration and regeneration of tissues are complex processes involving a large number of signaling mechanisms. In this study possible mechanisms of angiogenesis during tissue regeneration are presented. One of these mechanisms is the creation of a new capillary layer by angiogenesis. Previously it was believed that high and rapid levels of capillary growth are necessary for optimal healing of tissues. But several studies have demonstrated that a period of vascular regression occurs after the growth of blood vessels in a wound, and the number of the newly formed vessels decreases until the number of the blood vessels is equal to the density of the vascular network in the intact tissue. Reduced inflammation and angiogenesis are features of optimal healing.

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Application of new original ointments for treatment of burns with experimental pseudomonas infection

Year: 2011, volume 7 Issue: №2 Pages: 523-525
Heading: Surgery Article type: Original article
Authors: Shub G. М., Alipov V. V., Lebedev М. S., Dobreikin Е.А., Alipov N. V., Pronina E.A., Raikova S. V., Shapoval O. G.
Organization: Saratov State Medical University
Summary:

The research goal is to assess the efficacy of treatment with two new original ointments for burns with experimental pseudomonas infection. Burns have been caused by laser to three groups of white rats (20 animals in each group). In 3 days the wounds were infected with clinical strain of Pseudomonas aeruginosa. The wounds of the first group of rats were treated with ointments № 1, the wounds of the second group of rats — with ointments № 2. The third group did not receive any treatment. In 3, 10 and 14 days period the number of microbial cells in purulent discharge was studied. Then they were grown on meat-peptone agar. The number of colonies was counted. The number of cells in purulent discharge of treated wounds was less, than it was in purulent discharge of untreated wounds. The number of cells in purulent discharge of treated with ointments №1 wounds was higher, than it was in purulent discharge of treated with ointments №2 wounds. The article concludes that the local antimicrobial effect of both ointments is explained by their main components — some essential volatile oils and chloramphenicol

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Electromagnetic radiation influence on clinical course of experimental wound infection

Year: 2010, volume 6 Issue: №3 Pages: 500-503
Heading: Microbiology Article type: Original article
Authors: Pronina Е.А., Raykova S.B., Shvidenko I.G., Shub G.M.
Organization: Saratov State Medical University
Summary:

The article gives close attention to the study of electromagnetic radiation influence (EMR) at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide (150 GHz) and atmospheric oxygen (129 GHz) on the clinical course of experimental wound infection caused by antibiotic-sensitive and antibiotic-resistant strains of Pseudomonas aeruginosa. The panoramic spectrometric measuring complex, developed in Saratov Scientific Research Institute of Measuring Equipment was used while carrying out the research. Electromagnetic vibrations of extremely high frequencies were stimulated in this complex imitating the atmospheric oxygen and nitric oxide absorption and radiation molecular spectrum structure. The experiments proved the fact that exposure to radiation at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide and atmospheric oxygen had positive impact on the course of traumatic process

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Elective course material (Microbial communities)

Year: 2010, volume 6 Issue: №2 Pages: 245-247
Heading: Microbiology Article type: Original article
Authors: G.M. Shub, I.G. Shvidenko, E.A. Pronina, N.V. Beloborodova
Organization: Saratov State Medical University
Summary:

This article gives an outline of data concerning formation and functioning peculiarities of microbial communities in the form of biofilms. Peculiarities of metabolic processes and virulent properties of bacteria existing in biofilms which are connected to those processes are highlighted in the article. The necessity of taking into account these peculiarities when carrying out antibacterial therapy is also stated

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Changing of Bacteria Catalase Activity Under the Influence of Electro-Magnetic Radiation on a Frequency of Nitric Oxide Absorption and Radiation Molecular Spectrum

Year: 2009, volume 5 Issue: №3 Pages: 321-323
Heading: Microbiology Article type: Original article
Authors: E.A. Pronina, G.M. Shub, I.G Shvidenko
Organization: Saratov State Medical University
Summary:

The dynamics of catalase activity degree changing in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa is described under the influence of electro-magnetic radiation on a frequency of nitric oxide absorption and radiation molecular spectrum.
The panoramic spectrometric measuring complex, developed in Central Scientific Research Institute of measuring equipment Public corporation, Saratov, was used while carrying out the research. Electromagnetic vibrations of extremely high frequencies were stimulated in this complex imitating the structure of nitric oxide absorption and radiation molecular spectrum. The growth of activity of the mentioned enzyme of the strains under research was detected. The most significant changes were observed under 60-minutes exposure.

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Electromagnetic Radiation Influence With Molecular Spectrum Absorption and Nitric Oxide Radiation Frequency on Superoxide Dismutase Bacteria Activity

Year: 2009, volume 5 Issue: №2 Pages: 164-166
Heading: Microbiology Article type: Original article
Authors: E.A. Pronina, G.M. Shub, I.G. Shvidenko
Organization: Saratov State Medical University
Summary:

The dynamics of superoxide dismutase activity level under the influence of electromagnetic radiation with spectrum absorption and nitric oxide radiation frequency in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa has been described.
The panoramic spectrometric measuring complex, developed in Saratov Central Scientific Research Institute of Measuring Equipment Public Corporation has been used while carrying out the research. Electromagnetic vibrations of extremely high frequencies stimulated in this complex imitate the structure of molecular spectrum absorption and nitric oxide radiation. The activity of superoxide dismutase has been detected. The most significant changes have been observed under 45 and 60-minute exposition.

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