Saratov JOURNAL of Medical and Scientific Research

Shub G.M.

Saratov State Medical University, Head of Department of Microbiology, Virology and Immunology, Doctor of Medical Science, Professor

The study of antimicrobial activity of stonecrop extracts (Sedum maximum (L.) Hoffm., S. telephium I.)

Year: 2013, volume 9 Issue: №4 Pages: 640-643
Heading: Microbiology Article type: Original article
Authors: Plastun V.0., Raikova S.V., Durnova N.A., Shub G.M., Komarova E.A.
Organization: Saratov State Medical University
Summary:

The purpose of the work is to investigate the antimicrobial activity of aqueous alcoholic extract of large stonecrop and purple stonecrop. Material and Methods. The effects of large stonecrop (Sedum maximum (L.) Hoffm.) and purple stonecrop (S. telepium L.) on aqueous solution of alcoholic extracts on Staphylococcus aureus АТС С 209R, Pseu-domonas aeruginosa ATCC 27835, Escherichia coli ATCC 25922 have been studied by the method of double serial dilution followed by seeding on solid culture media. As a result of the work the minimum inhibitory concentrations for all extracts to all the studied cultures have been found. Stonecrop extracts have bactericidal action. Conclusion. Antimicrobial activity has been determined for the extracts of both species for all researched samples of microbial strains.

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Antimicrobial activity of peppermint essential oil (Mentha piperita L.)

Year: 2011, volume 7 Issue: №4 Pages: 787-790
Heading: Clinical Pharmacology Article type: Original article
Authors: Raikova S.V., Golikov A.G., Shub G.M., Durnova N.A., Shapoval O.G., Rakhmetova A.Yu.
Organization: Saratov State Medical University
Summary:

Рurposе. To study antimicrobial activity of fume of the essential oil of peppermint against gram-positive and gram-negative bacteria. Materials and methods: The screening study of antimicrobial activity of solutions of essential oil by disk-diffusion method and activity of essential oil fume of own preparation and pharmaceutical form of oil according to standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Esсherichia coli and 12 clinical strains of staphylococci (6 methicillin-resistant and 6 methicillin-sensitive) has been carried out. Results: Essential oil of own preparation and pharmaceutical form showed equal antimicrobial activity against strains of staphylococci. Essential oil of own preparation has been determined to reveal higher activity against gram-negative strains. Conclusion: Received data have proved the presence of antimicrobial activity against all strains of microorganisms and mostly against staphy-lococci

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Application of new original ointments for treatment of burns with experimental pseudomonas infection

Year: 2011, volume 7 Issue: №2 Pages: 523-525
Heading: Surgery Article type: Original article
Authors: Shub G. М., Alipov V. V., Lebedev М. S., Dobreikin Е.А., Alipov N. V., Pronina E.A., Raikova S. V., Shapoval O. G.
Organization: Saratov State Medical University
Summary:

The research goal is to assess the efficacy of treatment with two new original ointments for burns with experimental pseudomonas infection. Burns have been caused by laser to three groups of white rats (20 animals in each group). In 3 days the wounds were infected with clinical strain of Pseudomonas aeruginosa. The wounds of the first group of rats were treated with ointments № 1, the wounds of the second group of rats — with ointments № 2. The third group did not receive any treatment. In 3, 10 and 14 days period the number of microbial cells in purulent discharge was studied. Then they were grown on meat-peptone agar. The number of colonies was counted. The number of cells in purulent discharge of treated wounds was less, than it was in purulent discharge of untreated wounds. The number of cells in purulent discharge of treated with ointments №1 wounds was higher, than it was in purulent discharge of treated with ointments №2 wounds. The article concludes that the local antimicrobial effect of both ointments is explained by their main components — some essential volatile oils and chloramphenicol

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Electromagnetic radiation influence on clinical course of experimental wound infection

Year: 2010, volume 6 Issue: №3 Pages: 500-503
Heading: Microbiology Article type: Original article
Authors: Pronina Е.А., Raykova S.B., Shvidenko I.G., Shub G.M.
Organization: Saratov State Medical University
Summary:

The article gives close attention to the study of electromagnetic radiation influence (EMR) at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide (150 GHz) and atmospheric oxygen (129 GHz) on the clinical course of experimental wound infection caused by antibiotic-sensitive and antibiotic-resistant strains of Pseudomonas aeruginosa. The panoramic spectrometric measuring complex, developed in Saratov Scientific Research Institute of Measuring Equipment was used while carrying out the research. Electromagnetic vibrations of extremely high frequencies were stimulated in this complex imitating the atmospheric oxygen and nitric oxide absorption and radiation molecular spectrum structure. The experiments proved the fact that exposure to radiation at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide and atmospheric oxygen had positive impact on the course of traumatic process

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Elective course material (Microbial communities)

Year: 2010, volume 6 Issue: №2 Pages: 245-247
Heading: Microbiology Article type: Original article
Authors: G.M. Shub, I.G. Shvidenko, E.A. Pronina, N.V. Beloborodova
Organization: Saratov State Medical University
Summary:

This article gives an outline of data concerning formation and functioning peculiarities of microbial communities in the form of biofilms. Peculiarities of metabolic processes and virulent properties of bacteria existing in biofilms which are connected to those processes are highlighted in the article. The necessity of taking into account these peculiarities when carrying out antibacterial therapy is also stated

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