Saratov JOURNAL of Medical and Scientific Research

induced immunosuppression

Immuno-morphological features of the rat's thymus at the experimental administration of cytostatics

Year: 2016, volume 12 Issue: №1 Pages: 12-14
Heading: Macro- and Micromorphology Article type: Original article
Authors: Lebedinskaya Е.А., Lebedinskaya O.V., Godovalov А.Р., Prokudin V.S.
Organization: Perm State University of Medicine n.a. academician E.A. Vagner

The aim is to identify the changes in the rat's thymus structure under immunosuppression induced injection of cyclophosphamide with the use of morpho-histochemical and morphometric methods. Material and methods. For immunosuppression of animals cyclophosphamide at 100mg/kg body weight 4-fold with a 24-hour interval was injected intraperitoneally. Thymus serial paraffin sections were stained with hematoxylin and eosin, azure ll-eosin, methyl green pyronin according to Brachet, and periodic acid-Schiff before and after treatment with amylase. Results. Significant morphological changes in the thymus at rats with injection of cyclophosphamide were established. Morpho-histochemical changes in the rat's thymus characterize the immunosuppressive state of the experimental animals' organisms. Conclusion. It was shown the necessity of complex morphological studies including macrophage activity and characteristics of the subpopulation of mononuclear leukocytes spleen, changes in the structure of hematopoietic and lymphoid organs under immunosuppression induced by cyclophosphamide.

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