Saratov JOURNAL of Medical and Scientific Research

Fedyunin A.A.

Federal Medical and Biophysical Center n. a. A. I. Burnazyan, a Surgeon Surgical Department of Coordination of Organ Donation and (or) Human Tissue

Application evaluation of a polydimethylsiloxane low-viscosity for cryopreservation and radiation sterilization of human cadaveric vascular allografts.

Year: 2016, volume 12 Issue: №4 Pages: 662-670
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Fedyunin A.A., Kamyshnikov O.Yu., Vostrukhin S.V., Gorde'yev A.V., Paklina O.V., Kobzeva I.V., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, City Clinical Hospital n.a. S.P. Botkin
Summary:

Aim: to investigate potential cryo- and radioprotective properties of a polydimethylsiloxane of low viscosity to provide safe and reliable cryopreservation and radiation sterilization of human cadaveric vascular allografts. Material and methods. A consecutive experimental set-up was carried out to establish polydimethylsiloxane (further — PDMS) protective endocellular properties. For this purpose a high-densitymesenchymal stem cells suspension was cryopreserved with 10% DMSO solution; PDMS exocellular protective properties were assessed by cyclic cryoconservation of ^femoral artery segments, while PDMS potential radioprotectiveproperties were assessed by gamma irradiation — induced sterilization of 3 cryopreserved femoral artery segments with gross length about 3cm, and for control grafts a slightly changed protocol with glycerin was used. In this study the dynamic viscosity of all cryopreserved vessels was analyzed with using specially designed experimental device, also electron scanning microscopy with lanthanide cells staining and routine hematoxylin — eosin cell staining were applied. Results. We have demonstrated a prominentexocellular protective effect of PDMS confirmed by a SEM and histological results, and also its mediated radioprotective effect, in particular because of its safer preliminary cryopreservation procedure. Conclusion. Use of PDMS for a cryopreservation and gamma sterilization showed its rationality, however requires further modification of protocols and additional researches.

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A novel comprehensive approach for human vascular allografts cryopreservation and radiation sterilization for the tissue engineering industry

Year: 2015, volume 11 Issue: №4 Pages: 624-632
Heading: Genetics Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Saprykin V.P., Fediunin A.A., Paklina O.V., Gordeev A.V., Shakurov A.V., Belikov N.V., Khaidukova I.V., Burkov I.A., Antonov E.A., Ponomarev D.E., Kobzeva I.V., Savrasov G.V., Vorobyov G.V., Brumberg V.A., Nikitina V.A., Suchkova Y.B., Dobrovol'skaya E.I., Karaseva T.V., Gubarev K.K., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow State Technical University n.a. N.E. Bauman, City Clinical Hospital n.a. S.P. Botkin
Summary:

Aim: to verify new techniques for human cadaveric vascular allografts cryopreservation, thawing and sterilization for the tissue engineering purposes. We use polydimethylsiloxane (PDMS) as a well-known, promising coolant. This allowed us to completely omit any cryoprotective or vitrifying solutions. Using of PDMS also makes possible an applying these allografts directly after freezing and decellularization and also it will also provide an opportunity to develop secure protocols of tissue— engineered vascular conduits cryopreservation. Matherial and methods. After mathematical modeling of cooling process and its validation the experiment for sealed (isolated) freezing at low temperature conditions of 30 femoral arterial segments has been conducted. The segments were at least 10 cm in length and taken from 15 cadaveric donors in the age of 65-85 years. The freezing process was carried out using the abovementioned coolant— PDMS, and then physico-mechanical properties of these allografts were evaluated with the special Instron machine. According to the results obtained, a modeling of their sterilization conditions was conducted (the grafts were freezed). Results. By physico-mechanical properties validation and restricted histological analysis it was shown that there was an accordance between freezed/thawed allografts properties and native vessels. Conclusion. The abovementioned approach for allografts cryopreservation and thawing was efficient enough for further work in this direction.

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