Saratov JOURNAL of Medical and Scientific Research

Samoilov A.S.

Epidemiology, Professor, Doctor of Medical Science; A. S. Samoilov — Federal Medical and Biophysical Center n.a. A. I. Burnazyan, General Director, Assistant Professor, Doctor of Medical Science

Revisiting the Sports Medicine as a specialty in professional training of sports doctors

Year: 2017, volume 13 Issue: №4 Pages: 940-946
Heading: sports medicine Article type: Author's opinion
Authors: Razinkin S.M., Samoilov A.S., Petrova V.V.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

 This article discusses expediency of deducing sports medicine in a separate educational specialty. Also, there is a new concept of sports medicine offered. This concept includes the academic plan of postgraduate professional training of doctors in the direction of Sports Medicine specialty 08.31.39 Physical Therapy and Sports Medicine. Concept was developed by the department of experimental sports medicine of the A. I. Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency.

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Elaborating of a cryoconservation protocol of blood vessels for the tissue-specific matrices

Year: 2017, volume 13 Issue: №4 Pages: 891-900
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Rastorgueva A.A., Lomonosova Е.Е., Kobzeva I.V., Makhova A.E., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to create a protocol for complex cryoconservation of blood vessels in polydimethylsiloxane to create tissue-specific matrices. Material and Methods. The material ofthe study was the iliac arteries from postmortem donors, seized in the multi-organ donation, rejected for unclaimed and disposed of later. Polydimethylsiloxane was used as a coolant (PDMS) with a viscosity of 5-25 centistokes (Spectroplast, Russia). Bench testing of cooling and heating ofthe blood vessel in PDMS, testing of heating and cooling of PDMS up to operating temperatures (-80°C) in different ways and variants of its thermal insulation, assessment of different cooling rates ofthe vessel during cryoconservation, modeling of 3 types of working chambers for cryoconservation, sterilization and defrosting of up to 10 vessels, modeling of cryoconservation and thawing of vessels in PDMS under different conditions, selection of substrates from silicone and plastic for uniform fixation of vessels which are of size or size in the working chambers or without them. Results. The developed protocol allows cryopreserved from 1 to 10 or more vessels of all basic sizes both immediately after removal and after transportation to the laboratory, to vary the cooling rate from 1.5 to 300°C/min and above, to be used with different levels of laboratory equipment, as with liquid nitrogen, dry ice, and only with a medical freezer. Conclusion. The developed protocol allows to apply it in cases of complex cryoconservation of blood vessels to create tissue-specific matrices and can be recommended for preclinical testing.

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Physical factors impact on the status of regional hemodynamics and processes of microcirculation in patients with chronic bacterial prostatitis

Year: 2017, volume 13 Issue: №3 Pages: 537-540
Heading: Urology Article type: Original article
Authors: Samoylov A.S., Kulchitskaya D.B., Kolbakhova S.N., Goloborodko E.M., Bokov A.l., Konchugova T.V., Kiyatkin V.A.
Organization: Russian Scientific Center for Medical Rehabilitation and Balneology, State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The goal of the research is to study the impact of vacuum interference therapy on the state of the regional hemodynamics and the processes of microcirculation among patients with chronic bacterial prostatitis. Material and Meth- ods. The study included 90 patients with average age 32.5 years with chronic bacterial prostatitis in a latent phase of inflammation. The study was conducted on a comparative basis using three randomized groups of patients (n=30), comparable on all criteria, divided depending on the methods applied into two treatment groups and one control group. Besides, the originally assigned basic medication treatment was an obligatory component in all three groups. Results. After therapy course the more significant results in hemodynamic changes were shown in the group of patients who received vacuum-interference therapy. The second group, which received interference currents, there was also positive dynamics in the hemodynamic parameters, but to a smaller extent, while in the control group there were no vivid changes found in the studied parameters. Conclusion. The course treatment using vacuum interference therapy among patients with chronic bacterial prostatitis had a significant positive corrective effect on the system of hemodynamics.

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Risk of cataract in the context of acute and chronic exposure

Year: 2016, volume 12 Issue: №4 Pages: 678-684
Heading: radiation medicine Article type: Original article
Authors: Tukov A.R., Shafransky I.L., Kapitonova N.V., Prokhorova O.N., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: estimation of the risk of cataract using doses of different types of radiation. Material and methods. The study is carried out using the information database of the NP, recovery workers of the accident at the Chernobyl NP. Professional exposure and dose received during 30 km zone were used to calculate the risk. Results. The study shows the use of one of their parts of the total radiation dose of man, leads to obtaining of different levels of the risk of disease. Conclusion. Only use of a total radiation dose can lead to obtaining of the correct results of evaluating the risk of the emergence of the radiation- induced diseases.

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Application evaluation of a polydimethylsiloxane low-viscosity for cryopreservation and radiation sterilization of human cadaveric vascular allografts.

Year: 2016, volume 12 Issue: №4 Pages: 662-670
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Fedyunin A.A., Kamyshnikov O.Yu., Vostrukhin S.V., Gorde'yev A.V., Paklina O.V., Kobzeva I.V., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, City Clinical Hospital n.a. S.P. Botkin
Summary:

Aim: to investigate potential cryo- and radioprotective properties of a polydimethylsiloxane of low viscosity to provide safe and reliable cryopreservation and radiation sterilization of human cadaveric vascular allografts. Material and methods. A consecutive experimental set-up was carried out to establish polydimethylsiloxane (further — PDMS) protective endocellular properties. For this purpose a high-densitymesenchymal stem cells suspension was cryopreserved with 10% DMSO solution; PDMS exocellular protective properties were assessed by cyclic cryoconservation of ^femoral artery segments, while PDMS potential radioprotectiveproperties were assessed by gamma irradiation — induced sterilization of 3 cryopreserved femoral artery segments with gross length about 3cm, and for control grafts a slightly changed protocol with glycerin was used. In this study the dynamic viscosity of all cryopreserved vessels was analyzed with using specially designed experimental device, also electron scanning microscopy with lanthanide cells staining and routine hematoxylin — eosin cell staining were applied. Results. We have demonstrated a prominentexocellular protective effect of PDMS confirmed by a SEM and histological results, and also its mediated radioprotective effect, in particular because of its safer preliminary cryopreservation procedure. Conclusion. Use of PDMS for a cryopreservation and gamma sterilization showed its rationality, however requires further modification of protocols and additional researches.

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The question of a 'New Era in the Low Dose Radiation Epidemiology' approach

Year: 2016, volume 12 Issue: №4 Pages: 654-662
Heading: radiation medicine Article type: Review
Authors: Koterov A.N., Ushenkova L.N., Biryukov А.Р., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The historical and recent years data on cancer and/or leukemia rate increasing after exposure of people in low dose (up to 100 mGy) radiation with a low LET, attributiveness of effects and the possibility of their experimental confirmation were considered. Previously, information about the carcinogenic effects of low doses was not clearly interpreted due to the presence of uncertainty, biases and confounders. The biological mechanism was also absent. In the last 5-7 years the situation has changed dramatically: more significant data were obtained: irradiation at computed tomography in dentistry, for residents of a high natural background radiation, and others. Simultaneously it was obtained radiobio-logical data on increased DNA double strand breaks level after exposure to doses from few milligrays and it can be considered as a possible molecular mechanism of these effects. As a result, was declared 'New Era in the Low Dose Radiation Epidemiology' (Kitahara С M., et al., 2015), which can lead to costs in terms of tightening of radiation risks and to fear of medical exposure. The conducted in the review analysis of recent epidemiological and radiobiological evidence suggests, however, that there is no unambiguous evidence of attributed to radiation carcinogenic effects, identified during the 'New Epidemiology of Low Doses', and there is no a proven molecular mechanism that could provide biological plausibility of such effects.

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Optimal frequency characteristics of impulse laser in treatment of patients with atherosclerosis of blood vessels in lower extremities

Year: 2016, volume 12 Issue: №4 Pages: 599-601
Heading: Psysiotherapy Article type: Original article
Authors: Samoilov A.S., Kulchitskaya D.B., Kolbakhova S.N.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The research goal is to assess on a comparative basis the effects of the infrared low-intensity laser emission with the frequency of 80 Hz and 1500 Hz on the state of microcirculation and peripheral blood circulation among 30 patients with atherosclerosis of the vessels of the lower extremities. Material and Methods. The patients were divided into 2 groups: in the 1st group (15 patients) patients received pulse infrared low-intensity laser radiation with the frequency of 80 Hz; in the 2nd group (15 patients) infrared low-intensity laser radiation with the frequency of 1500 Hz was used. Results. As a result of the conducted investigation, it was found that optimum pulse repetition rate was 80 Hz because more significant changes in the system of microcirculation and peripheral blood circulation among patients with atherosclerosis of the vessels of lower extremities were revealed. Conclusion. The optimal frequency pulse is determined to be 80 Hz.

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Comprehensive radiation survey and public health assessment in the vicinity of NPP

Year: 2015, volume 11 Issue: №4 Pages: 633-637
Heading: Hygiene Article type: Original article
Authors: Samoilov A.S., Shandala N.K., Korenkov I.P., Romanov V.V., Filonova A.A., Novikova N.Ya.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim. For many years, SRC-FMBC carried out comprehensive monitoring of radiation-hygienic situation in order to assess the dynamics of the measurement of the environmental media, public health and to develop health and epidemiological measures to assure the public welfare. Matherial and methods. The radiation-hygienic conditions of the environment are assessed by comparing the obtained results with the regional background values. Result. The radionuclide concentrations in the environmental media within the NPP supervision area do not exceed typical regional background values. We studied the radionuclide concentrations in some foodstuffs of plant and animal origin, drinking water and other environmental media. The concentrations of 137Cs and 90Sr in foodstuffs are the same as in the similar foodstuffs from other regions and the whole Russia. This is due to global precipitation of 137Cs and 90Sr following the nuclear weapons tests and accident at the Chernobyl NPP of 1986. Conclusion. According to analysis of the main
health indicators of the public living around the NPP, general state of the adult and children public is healthy. There is no impact of the NPP on negative trends in some changing indicators of the adult and children public health.

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