Saratov JOURNAL of Medical and Scientific Research

self-organization

Different reaction of core histones H2A and H2B to the red laser radiation

Year: 2017, volume 13 Issue: №3 Pages: 469-472
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Brill G.E., Egorova A.V., Bugaeva I.O., Ushakova O.V., Matyushkina O.L.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Aim: to investigate the influence of red laser irradiation on the processes of self-assembly of core histones H2A and H2B. Material and Methods. Solutions of human histone proteins were used in the work. Self-assembly was studied by the method of wedge dehydration. Image facies analysis consisted in their qualitative characterization and calculation of quantitative indicators with subsequent statistical processing. Results. It was established that linearly polarized laser light of the red region of the spectrum (A=660 nm, 1 J/cm2) significantly modifies the process of self-assembly of core histone H2B, while the structure of the facies of H2A histone changing to a lesser extent. Conclusion. Red laser radiation influences on the on the processes of self-assembly of core histones H2A and H2B. There is a differential sensitivity of different classes of histones to laser action. Histone proteins used in the experiments are present in the form of aqueous salt solutions. Red light realizes the effect seems to be due to the formation of singlet oxygen by direct laser excitation of molecular oxygen.

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Effect of low-intensity electromagnetic radiation on structurization properties of bacterial lipopolysaccharide

Year: 2013, volume 9 Issue: №4 Pages: 637-640
Heading: Microbiology Article type: Original article
Authors: Brill G.E., Egorova А.V., Bugaeva I.О, Dubovitsky S.A., Vlaskin S.V.
Organization: Research and production firm “Telemak”, Russia, Saratov, Saratov State Medical University
Summary:

Purpose: to investigate the effects of low-intensity electromagnetic radiation on the process of dehydration self-organization of bacterial lipopolysaccharide. Materials and Methods. The method of wedge dehydration has been used to study the structure formation of bacterial lipopolysaccharide. Image-phases analysis included their qualitative characteristics, as well as the calculation of quantitative indicators, followed by statistical analysis. Results. UHF-Radiation (1GHz, 0,1 uW/cm2, 10 min) has led to the changes in the suspension system of the LPS-saline reflected in the kinetics of structure formation. Conclusion. 1 GHz corresponds to the natural frequency of oscillation of water clusters and, presumably, the effect of UHF on structure of LPS mediates through the changes in water-salt environment. Under these conditions, properties of water molecules of hydration and possibly the properties of hydrophobic and hydrophilic regions in the molecule of LPS, which can affect the ability of toxin molecules to form aggregates change. Therefore the lipopolysaccharide structure modification may result in the change of its toxic properties.

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