Saratov JOURNAL of Medical and Scientific Research

Egorova A.V.

Saratov State Medical University n.a. V. I. Razumovsky, Department of Histology, Assistant Professor, Candidate of Medical Science

Evaluation of the efcacy of neuroprotective therapy after endovitreal surgery for rhegmatogenous retinal detachment

Year: 2019, volume 15 Issue: №2 Pages: 460-464
Heading: Ophtalmology Article type: Original article
Authors: Egorov A.V., Egorov V.V., Smoliakova G.P.
Organization: The Khabarovsk branch of the S. Fedorov Eye Microsurgery Federal State Institution

 Purpose: to develop optimal approaches to the use of cytofavin for neuroprotection and to evaluate their efective-ness in patients after endovitreal surgery for rhegmatogenous retinal detachment (RRD) with anatomically complete retinal attachment. Material and Methods. 68 people (68 eyes) with primary RRD. Three-port vitrectomy with silicone oil tamponade in the vitreous cavity and its subsequent removal were performed. The patients were divided into 2 groups: the main group (38 people who received cytofavin added to standard therapy), and the control group (30 people with standard therapy only). Depending on visual prognosis calculated before surgery, the main and control groups consisted of patients with tolerable and low levels of visual prognosis. We used laser doppler fowmetry (to register microcirculation index (MI) and microcirculation efciency index (MEI)). Morphological signs of macular ischaemia were recorded by optical coherence tomography angiography (OCT-angiography). Results. Regardless of visual prognosis, the response dynamics of the chorioretinal microcirculation in patients was unidirectional. In patients of the main group 6 months after the surgery, more signifcant positive changes in growth of amplitude of MI and MEI, as well as positive dynamics of OCT-angiography were observed. Conclusion. Including cytofavin neuroprotection in postoperative treatment in patients after

2019_02-1_460-464.pdf310.21 KB

Erythrocyte system in elderly: ageing aspects

Year: 2018, volume 14 Issue: №2 Pages: 341-345
Heading: Gerontology and geriatrics Article type: Original article
Authors: Malinova L.I., Denisova Т.Р., Lipatova Т.Е., Khvostikova D.E., Yegorova A.V.
Organization: Saratov State Medical University

The purpose of the study was an assessment of erythrocyte system age-dependent characteristics in elderly population dwelling in the same territory during the time period equal to the average life span in the region. Material and Methods. We perform the compare of hematological parameters of 800 patients with hypertension and coronary heart disease hospitalized during 25-years period. Results. In senile patients, red blood cells and hemoglobin levels signifi- cantly decrease, but platelet characteristics remain intact. Revealed differenced are similar during all 25-year period of observation. Conclusion. Over the past 25 years, the trend of age-related decline in the number of red blood cells and hemoglobin has been preserved. The average hemoglobin content in erythrocyte (MCH) during the aging process tends to decrease, which has become statistically significant in recent years. The principal difference of the obtained data from the results of obtained 25 years ago is the absence of an age-related increase in the volume of erythrocytes and a higher level of the average concentration of hemoglobin in the erythrocyte.

2018_2-1_341-345.pdf291.29 KB

Interaction of atherosclerosis and ageing: is there any discussion questionable?

Year: 2018, volume 14 Issue: №2 Pages: 322-327
Heading: Gerontology and geriatrics Article type: Author's opinion
Authors: Denisova Т.Р., Lipatova Т.Е., Alipova L.N., Yegorova A.V.
Organization: Saratov Regional Hospital for Veterans of Wars, Saratov State Medical University

Age-related diseases and their link to involutionary processes in human organism remain as one of the burning problems in internal medicine. The article focuses on the discussable questions of the most common mechanisms of atherogenesis and ageing, their similarity and difference. Both processes mutually burdens each other, bringing the "fading" of the organism closer.

2018_2-1_322-327.pdf296.51 KB

Different reaction of core histones H2A and H2B to the red laser radiation

Year: 2017, volume 13 Issue: №3 Pages: 469-472
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Brill G.E., Egorova A.V., Bugaeva I.O., Ushakova O.V., Matyushkina O.L.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov

Aim: to investigate the influence of red laser irradiation on the processes of self-assembly of core histones H2A and H2B. Material and Methods. Solutions of human histone proteins were used in the work. Self-assembly was studied by the method of wedge dehydration. Image facies analysis consisted in their qualitative characterization and calculation of quantitative indicators with subsequent statistical processing. Results. It was established that linearly polarized laser light of the red region of the spectrum (A=660 nm, 1 J/cm2) significantly modifies the process of self-assembly of core histone H2B, while the structure of the facies of H2A histone changing to a lesser extent. Conclusion. Red laser radiation influences on the on the processes of self-assembly of core histones H2A and H2B. There is a differential sensitivity of different classes of histones to laser action. Histone proteins used in the experiments are present in the form of aqueous salt solutions. Red light realizes the effect seems to be due to the formation of singlet oxygen by direct laser excitation of molecular oxygen.

2017_03_469-472.pdf413.56 KB

Effect of low-intensity electromagnetic radiation on structurization properties of bacterial lipopolysaccharide

Year: 2013, volume 9 Issue: №4 Pages: 637-640
Heading: Microbiology Article type: Original article
Authors: Brill G.E., Egorova А.V., Bugaeva I.О, Dubovitsky S.A., Vlaskin S.V.
Organization: Research and production firm “Telemak”, Russia, Saratov, Saratov State Medical University

Purpose: to investigate the effects of low-intensity electromagnetic radiation on the process of dehydration self-organization of bacterial lipopolysaccharide. Materials and Methods. The method of wedge dehydration has been used to study the structure formation of bacterial lipopolysaccharide. Image-phases analysis included their qualitative characteristics, as well as the calculation of quantitative indicators, followed by statistical analysis. Results. UHF-Radiation (1GHz, 0,1 uW/cm2, 10 min) has led to the changes in the suspension system of the LPS-saline reflected in the kinetics of structure formation. Conclusion. 1 GHz corresponds to the natural frequency of oscillation of water clusters and, presumably, the effect of UHF on structure of LPS mediates through the changes in water-salt environment. Under these conditions, properties of water molecules of hydration and possibly the properties of hydrophobic and hydrophilic regions in the molecule of LPS, which can affect the ability of toxin molecules to form aggregates change. Therefore the lipopolysaccharide structure modification may result in the change of its toxic properties.

2013-04_637-640.pdf1.35 MB