Saratov JOURNAL of Medical and Scientific Research

Belova S.V.

Saratov Research Institute of Traumatology and Orthopaedics, Department of Fundamental and Clinical Experimental Investigations, Senior Research Assistant, Candidate of Biological Science

The peculiarities of bone and cartilage tissue remodeling in patients after hip joint replacement

Year: 2017, volume 13 Issue: №3 Pages: 723-727
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Gladkova Е.М., Karyakina E.V., Tsareva Е.Е., Persova Е.А., Belova S.V.
Organization:
Summary:

The aim: to study the peculiarities of structural and metabolic state of bone and cartilage tissue in patients in the first 12 months after total hip replacement with respect to their immune state and lipid peroxidation activity. Material and Methods. Significant laboratory predictors of initial signs of aseptic instability were singled out allowing estimating the peculiarities of bone tissue remodeling as well as humoral and cellular immunity state. Results. The obtained results demonstrated that impairments in T-lymphocyte system in patients with initial signs of aseptic instability of en-doprosthesis were accompanied by reduction of its total number (CD3+), among that due to the population of cytotoxic (CD3+CD8+) cells with simultaneous increase of T-helpers (CD3+CD4+) number. Activation of serum proosteoclasto-genic factors (TNF-a and IL-1(3) was noticed on the background of anti-inflammatory humoral immunity failure (IL-10). Conclusion. The estimation of cellular immunity state, content of pro- and anti-inflammatory cytokines in blood serum combined with the bone tissue state markers and indexes of lipid peroxidation and antioxidant system as well as dual-energy absorptiometry allows identifying patients with high risk of further development of aseptic instability in hip joint implant in the period of up to 12 months postsurgically which can be useful while planning prevention management of implant or its components instability.

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Prognostic opportunities for infectious inflammatory complications in primary endoprosthesis of large joints

Year: 2017, volume 13 Issue: №3 Pages: 715-719
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Belova S.V., Mamonova I.A., Puchinyan D.M., Babushkina I.V., Gladkova E.M., Adilov R.G.
Organization: Saratov State Medical University
Summary:

The aim: to study prognostic and diagnostic opportunities for infectious inflammatory complications of primary endoprosthesis of hip and knee joints on the basis of cellular immunity indices. Material and Methods. 174 patients with large joint osteoarthrosis aged 61 ±13 yrs which had undergone total joint replacement were enrolled into the study. The experimental group consisted of 22 patients with infectious inflammatory complications emerging from 6 up to 12 months postsurgically. The comparison group included 152 patients with favorable postsurgical period. We carried out WBC differentiation by subpopulations (lymphocytes, monocytes, granulocytes) and their immunophenotyping. 40 conventionally healthy individuals comprised the control group. The outcomes were estimated using the worked-out score system. Results. In patients with favorable postsurgical period total value of indices equaled not more than 4-6 points. In all the other cases the risk of infectious inflammatory complications is significantly higher and reaches its maximal values in patients with 10-12 points. Conclusion. The assessment of laboratory indices reflecting immune response of the body allows estimating the risk of infectious inflammatory postsurgical complications and diagnosing them in III grade large joint osteoarthorisis patients after total replacement.

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Comparative study of complex powdery preparations for soft tissue regeneration

Year: 2017, volume 13 Issue: №3 Pages: 705-710
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Babushkina I.V., Gladkova Е.М., Mamonova I.A., Belova S.V.
Organization:
Summary:

The aim: comparative study of regenerative activity of powdery preparations for local treatment of soft tissue injuries developed from copper and silver nanoparticles in comparison with traditional powders containing antibiotics within the conditions of an experimental conditionally aseptic wound. Material and Methods. The investigation was carried out on 80 albino rats with an experimental conditionally aseptic wound. Copper and silver nanoparticles obtained by the plasma-chemical method, sterile starch, zinc oxide, Baneocin were used. Planimetric and bacteriological research methods were applied. Results. Treatment with the developed preparations in comparison with the traditional ones containing antibiotics supplied more effective course of reparative processes as evidenced by the data of the planimetric indices. Conclusion. Besides the developed preparations due to the evident antibacterial action supplied reliable prevention of the secondary infection which is a compulsory condition for the course of reparative regeneration processes. The use of metal nanoparticles for this purpose allowed to prevent the adverse reactions which are characteristic of similar preparations containing antibiotics.

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The characteristics of cellular element of immunity before and after endoprosthesis of large joints

Year: 2016, volume 12 Issue: №2 Pages: 182-185
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Mamonova I.A., Gladkova Е.М., Uljanov V.Yu., Babushkina I.V., Belova S.V., Puchinyan D.M., Kuzmin E.S., Bezrokov L.Kh.
Organization:
Summary:

The aim of the research is to study the indices of cellular immunity in patients before and after hip joint endoprosthesis. Materials and methods. The indices of cellular element of immunity (quantitative and qualitative composition of T-lymphocytes (CD3+), T-helpers (CD3+CD4+), cytotoxic T-lymphocytes (CD3+CD8+), B-lymphocytes (CD3-CD19+), natural killers (CD3-CD16+CD56)) in 20 patients with post-traumatic osteoarthrosis of III grade preoperatively and in the dynamic follow-up on the 3rd, 7th and 21st day post-surgically. Results. Patients with osteoarthrosis showed expressed immunological impairments both before and after endoprosthesis mostly affecting T-cellular immunity element and manifesting in immunoregulatory subpopulations imbalance as well as in T-suppressors decrement, T-helpers increment and NK-cells enlargement in patients after endoprosthesis. Conclusion. Patients with osteoarthrosis showed expressed immunological impairments both before and after joint endoprosthesis. Surgical intervention resulted in the aggravation of the pre-surgical disturbances in immunodeficiency.

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Cytologic assessment of dynamics of reparative processes in the experimental conditionally aseptic wound under the action of complex preparation based on chitosan and metal nanoparticles

Year: 2013, volume 9 Issue: №4 Pages: 629-632
Heading: Macro- and Micromorphology Article type: Original article
Authors: Konyuchenko Е.А.. Zhandarova L.F., Babushkina I.V., Gladkova E.V., Mamonova I.A., Belova S.V., Karyakina E.V.
Organization: Saratov State Medical University
Summary:

Objective: assessment of dynamics of reparative processes with the help of cytological examination of the preparations obtained from a conditionally aseptic wound under the action of a complex preparation based on biopolymer chitosan and metal nanoparticles. Material and Methods. A conditionally aseptic wound was simulated in 40 white outbred rats. In comparison and experimental groups cytologic assessment of dynamics of reparative processes was carried out. A number of neutrophilic leukocytes and fibroblastic row perl 00 cells in the preparations is recorded on the 3rd, 5th, 7th, 10th, 14th day from the moment of formation of the wound with the use of the immersion objective. Results. It is established, that in the comparison group the cytologic pattern of impression smear was characterized by decrease in neutrophilic leukocytes and increase in fibroblastic row cells by the 14th day from the moment of formation of the wound. In the experimental group (the use of a complex preparation) more intensive reduction of neutrophilic leukocytes and a significant increase in fibroblastic row cells by the 10th day and a complete healing by the 14th day from the moment of formation of the wound has been determined. Conclusion. When treated by a complex preparation based of biopolymer chitosan and metal nanoparticles earlier occurrence of fibroblastic row cells in preparations and a significant decrease in quantity of neutrophilic leukocytes have been observed.

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