Saratov JOURNAL of Medical and Scientific Research

Shapoval O.G.

Saratov State Medical University n.a. VI. Razumovsky, Senior Teacher of Department of Microbiology, Virology and Immunology PhD

Department of Microbiology of Saratov State Medical University name after V. I. Razumovsky: the origins and the history of development (1918-2018)

Year: 2019, volume 15 Issue: №1 Pages: 127-131
Heading: Scientific schools and memorable dates Article type: Short message
Authors: Mikerov A..N, Raykova S.V., Shvidenko I.G., Khodakova N.G., Soboleva E.F., Shapoval O.G., Allyanova M.S., Kutyrev V.V.
Organization: Saratov State Medical University
Summary:

Department of Microbiology, Virology and Immunology is one of the oldest departments of Saratov State Medical University n.a. V. I. Razumovsky. It has the rich historical experience in the organizing of studies and professional activities of students, which is an integral part in training and education of a modern doctor.

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Assessment of antimicrobial activity of polyazolidinammonium modified by iodine hydrate ions depending on its physical and chemical characteristics.

Year: 2015, volume 11 Issue: №3 Pages: 255-257
Heading: Microbiology Article type: Original article
Authors: Vakaraeva M.M., Uljanov V.Yu., Nechaeva O.V., Luneva I.0., Tikhomirova E.l., Shapoval O.G., Zayarskiy D.A.
Organization: Saratov State Medical University, Saratov state university
Summary:

Aim: to study antimicrobial activity of variants of polymeric compound polyazolidinammonium modified by iodine hydrate-ions (PAAG-M), depending on the polymeric chain length and concentration of the iodine hydrate-ions against standard strains of gram-positive and gram-negative bacteria as well as microscopic fungi. Materials and methods. Minimum inhibitory concentrations of all variants of the polymeric compound for tested microbial strains have been determined by using serial dilution method. Results. High sensitivity of Eschehchia coli 113-13 and Pseudomonas aeruginosa ATCC 27853 was shown to the variants of PAAG-M with molecular weight

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Antimicrobial activity of peppermint essential oil (Mentha piperita L.)

Year: 2011, volume 7 Issue: №4 Pages: 787-790
Heading: Clinical Pharmacology Article type: Original article
Authors: Raikova S.V., Golikov A.G., Shub G.M., Durnova N.A., Shapoval O.G., Rakhmetova A.Yu.
Organization: Saratov State Medical University
Summary:

Рurposе. To study antimicrobial activity of fume of the essential oil of peppermint against gram-positive and gram-negative bacteria. Materials and methods: The screening study of antimicrobial activity of solutions of essential oil by disk-diffusion method and activity of essential oil fume of own preparation and pharmaceutical form of oil according to standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Esсherichia coli and 12 clinical strains of staphylococci (6 methicillin-resistant and 6 methicillin-sensitive) has been carried out. Results: Essential oil of own preparation and pharmaceutical form showed equal antimicrobial activity against strains of staphylococci. Essential oil of own preparation has been determined to reveal higher activity against gram-negative strains. Conclusion: Received data have proved the presence of antimicrobial activity against all strains of microorganisms and mostly against staphy-lococci

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Application of new original ointments for treatment of burns with experimental pseudomonas infection

Year: 2011, volume 7 Issue: №2 Pages: 523-525
Heading: Surgery Article type: Original article
Authors: Shub G. М., Alipov V. V., Lebedev М. S., Dobreikin Е.А., Alipov N. V., Pronina E.A., Raikova S. V., Shapoval O. G.
Organization: Saratov State Medical University
Summary:

The research goal is to assess the efficacy of treatment with two new original ointments for burns with experimental pseudomonas infection. Burns have been caused by laser to three groups of white rats (20 animals in each group). In 3 days the wounds were infected with clinical strain of Pseudomonas aeruginosa. The wounds of the first group of rats were treated with ointments № 1, the wounds of the second group of rats — with ointments № 2. The third group did not receive any treatment. In 3, 10 and 14 days period the number of microbial cells in purulent discharge was studied. Then they were grown on meat-peptone agar. The number of colonies was counted. The number of cells in purulent discharge of treated wounds was less, than it was in purulent discharge of untreated wounds. The number of cells in purulent discharge of treated with ointments №1 wounds was higher, than it was in purulent discharge of treated with ointments №2 wounds. The article concludes that the local antimicrobial effect of both ointments is explained by their main components — some essential volatile oils and chloramphenicol

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Doxorubicine and antibiotics influence on dynamics of population development of Staphylococcus aureus and Escherichia coli

Year: 2010, volume 6 Issue: №1 Pages: 040-042
Heading: Microbiology Article type: Original article
Authors: O.G. Shapoval
Organization: Saratov State Medical University
Summary:

Infectious complications in oncologic patients often occur as a result of antitumor therapy that demands the prescription of antimicrobic preparations. one of the main infectious agents in oncology is Staphylococci and Escherichia coli. The aim of this work is to study doxorubicine and ceftriaxon influence on dynamics of population development of strains of Staphylococcus aureus, doxorubicine and amikacine - on dynamics of population development of strains of Escherichia coli. Five strains of each type (one-standard and four-clinical) cultivated in meatpeptone broth, which contains non-inhibitory concentrations of experimental preparations. in any period of time after the sowing the measurement of optical density of growing cultures has been carried out. it has been determined, that doxorubicine in non-inhibitory concentrations increases overwhelming effect of these antibiotics on dynamics of population development of experimental strains

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АНТИМИКРОБНАЯ АКТИВНОСТЬ ПРОИЗВОДНЫХ ЦИКЛОГЕКСАНОЛА И 5 – НИТРОТИОФЕНОВОГО АЛЬДЕГИДА (RUS)

Year: 2006, volume 2 Issue: №2 Pages: 63-66
Heading: Microbiology Article type: Original article
Authors: O.G. Shapoval, L.A. Popaduk, Yu.A. Fomina, A.G. Golikov
Organization: Saratov State Medical University, Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

Antimicrobial activity of new heterocyclic compounds on standart strains grampositive Staphylococcus aureus ATCC 29213 and gramnegative – Esherichia coli ATCC 25992 (M-17) Proteus mirabilis 20 was study/
Compounds №6 and №7 have shown high overwhelming action (minimum inhibitory concentration – MIC 39,58 +/- 6,8mkg/ml accordingly. This lower exceeds MIC of ceftrixon in two times ( MIC 39,58+/- 6,8 mkg/ml) and is near to MIC of furamag (11,51/-0,99 mkg/ml).
Compounds №6 activity on methicillinsesitive strains Staphylococcus aureus was two times higher (MIC 12,4 /- 4,34) than on methicillinstable strains Staphylococcus auerus (MIC 25,6+/- 5,02 mkg/ml). Antimicrobial action of compound №7 was the same both on methicillinstable strains (MIC 17,3+/-1,77 mkg/ml) and on methicillinsensitive strains (MIC 12,53+/-3,78 mkg/ml) Staphylococcus aureus. The same regularity was mark for chemical furamag. Activity of chemical ceftriaxon on methicillinstable strains Staphylococcus aureus was greatly lower.

INFLUENCE OF DOXORUBICIN ON ADHESIVE PROPERTIES OF E.COLI

Year: 2008, volume 4 Issue: №3 Pages: 33-36
Heading: Microbiology Article type: Original article
Authors: O.G. Shapoval
Organization: Saratov State Medical University
Summary:

Influence ofantineoplastic drug doxorubicin and amikacin, the aminoglycoside family on adhesive activity of Escherichia coli was studied. Antimicrobialactivity(minimum inhibitory concentration-MIC) ofboth drugs against experimental strains using serial two-fold dilution method was determined. Susceptibility of E.coli to amikacin in the presence of Sand j MIC doxorubicin was studied. After 10 passages in beef-extract broth with constant and increasing doxorubicin concentrations in the presence of Sand j MIC doxorubicin, the adhesive activity of initial and passage variants according to theirability to absorb human erythrocytes 1(0) Rh+ was determined. Itwas observed that experimental strains were susceptible to amikacin (MIC 1,5-6,2 mkg/ml) butwere resistantto doxorubicin (MIC 1000 mkg/ml). Subinhibitory concentrations of this cytostatic (S and j MIC) raised the sensitivity of experimental strains to amikacin and differently effected on adhesive activity of passage variants of E.coli.

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