Saratov JOURNAL of Medical and Scientific Research

Peritoneal fluid cells reactivity in modeling of aseptic inflammation

Year: 2020, volume 16 Issue: №3 Pages: 810-813
Heading: Рathophysiology Article type: Original article
Authors: Godovalov А.Р., Morozov I.A., Karpunina T.N.
Organization: Perm State University of Medicine n.a. Academician Ye.A. Vagner of Ministry of Health of the Russian Federation

The aim of investigation was to evaluate the characteristics of the response of rat peritoneal leukocytes and mast cells (MC) when using experimental aseptic inflammation. Material and Methods: Rats of the 1st group (n=7) were intra-peritoneally injected with 1 % thioglycolate broth, animals ofthe2nd(n=7)— the same broth, diluted 1:10. Control group rats (n=8) received an equal volume of saline. After removing animals from the experiment in the peritoneal fluid, the number of leukocytes was counted and their viability was evaluated. MC degranulation was detected by toluidine blue technique. All MCs were divided according to the degree of degranulation. Results: A model of aseptic inflammation of the peritoneum was reproduced. It was found that the number of leukocytes of peritoneal fluid, especially the number of non-viable cells, increases by more than 2 times with an increase in the concentration of the eluting agent (p<0.05). In experimental animals, the number of MCs with signs of degranulation increased to 89.5±2.2% compared with their level in the control group (49.0±3.9%; p<0.05). Conclusion: With the introduction of thioglycolate broth into the rats peritoneal cavity, an adequate model of inflammation is reproduced, suitable for studying the activity of different types of cells and humoral factors. The clinical picture of inflammation varies depending on the degree of degranulation of MC, which confirms their participation in the development of the pathological process.

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