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Application of cell technologies in thermal burn damage to skin (Practical experience in State Research Center— Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency of Russia)

Year: 2019, volume 15 Issue: №4 Pages: 999-1004
Heading: Тhematic supplement Article type: Original article
Authors: Samoilov A.S., Astrelina Т.А., Aksenenko A.V., Kobzeva I.V., Suchkova Yu.B., Nikitina V.A., Usupzhanova D.Yu., Brunchukov V.A., Brumberg V.A., Rastorgueva A.A., Makhova A.E., Karaseva T.V., Lomonosova Е.Е., Dobrovolskaya E.l., Udalov Yu.D.
Organization: Moscow Helmholtz Research Institute of Eye Diseases, State Research Center— Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to present the results of the use of autologous regenerative cells of adipose tissue (AT) in treatment of patients with deep thermal burns. Material and Methods. The use of cellular technologies was carried out on 5 patients (4 men and 1 woman) aged 23-45 years, with deep thermal burns of III—IV degree of various etiology and localization, lesions from 1 % to 80%. Patients received conventional conservative therapy for burn wounds. After stabilization of the patient's general condition and positive dynamics of the local process in the wound during the planned surgical treatment of burn surfaces, the biological material of the AT was obtained. The volume of lipoaspirate AT was 195.0±74.2 ml. For clinical use of AT, the stromal-vascular fraction (SVF) of AT was obtained. The patients received intradermal injection of autologous SVF in the amount of 10 million to 50 million cells in a volume of 5 ml in 10-15 points around the damage to skin. Results. Upon examination (2.5 months after the point of introduction of SVF of AT), patients showed good cosmetic and functional results. The skin is soft, elastic, there are no rough hypertrophic and keloid scars, no contractures. Scars do not stand out. Movement in the joints is saved. Conclusion. The use of SVF AT in the early periods after injury contributed to activation of reparative processes in the dermis, which reduced the local inflammatory response, accelerated epithelialization, restoring skin elasticity with a decrease in the severity of fibrous scars and resulted in the lack of mobility in the joints.

Keywords: autologous regenerative adipose tissue cells, burn injury, cellular therapy

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All articles of the authors: Aksenenko A.V., Astrelina T.A. , Brumberg V.A. , Brunchukov V.A., Dobrovol'skaya E.I., Karaseva T.V. , Kobzeva I.V. , Lomonosova E.E. , Makhova A.E. , Nikitina V.A. , Rastorgueva A.A. , Samoilov A.S. , Suchkova Y.B. , Udalov U.D. , Usupzhanova D.Yu.

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