Saratov JOURNAL of Medical and Scientific Research

Comparative analysis of long noncoding RNA NEAT1, MALAT1, GAS5, RoR, HOTAIR in the blood of prostate cancer patients before and after radiotherapy

Year: 2019, volume 15 Issue: №4 Pages: 1004-1008
Heading: Тhematic supplement Article type: Original article
Authors: Shulenina L.V., Mikhailov V.F., Neznanova M.V., Saleeva D.V., Zasukhina G.D.
Organization: State Research Center— Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, The Vavilov Institute of General Genetics of Russian Academy of Sciences

Purpose: to study the content of long non-coding RNA regulating the activity of the tumor suppressor P53 in the peripheral blood of prostate cancer patients negatively. Material and Methods. The object of study was venous blood of patients diagnosed with prostate cancer (from T1N0M0 to T3N0M0 stages), collected before and after the full course of radiotherapy. The content of long noncoding RNA NEAT1, MALAT1, GAS5, RoR, HOTAIR in the blood of cancer patients was determined using the real-time PCR method combined with reverse transcription and specific primers. The data are presented as the median and quartiles relative to the median of indicators of the control group healthy donors, conditionally taken as 1. Statistical processing of the results was carried out using the non-parametric Mann — Whitney test. Results. An increase of the content of NEAT1, MALAT1, GAS5, HOTAIR in the blood of prostate cancer patients in comparison with the group donors was found. It was revealed that the course of radiotherapy did not affect the magnitude of the indicators we studied. Conclusion. Analysis of data and reference literature suggests that NEAT1, MALAT1 and HOTAIR levels in the blood of prostate cancer patients reflect their levels in tumor cells. NEAT1, MALAT1, GAS5 and HOTAIR can be potential markers for cancer diagnosis and treatment.

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