Saratov JOURNAL of Medical and Scientific Research

Bioflm formation by Staphylococcus strains isolated from various biological materials at infectious complications following total knee replacement

Year: 2019, volume 15 Issue: №2 Pages: 261-266
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Babushkina I.V., Bondarenko A.S., Mamonova I.A., Ulyanov V.Yu.
Organization: Saratov State Medical University

Objective: to study Staphylococcus spp. Strains bioflm forming ability in vitro. These strains are infectious agents for implant-associated infection in patients following total knee replacement depending on the biomaterial under study. Material and Methods. Bioflm forming ability in vitro of 275 Staphylococcus spp. strains has been studied. Bioflm formation was performed using modiefed method of G. D. Christensen. Results. Bioflm forming ability was discovered in 51.2 % of the studied strains, at that it was reliably (p<0.05) higher forcoagulase-positive staphylococci compared to coagulase-negative ones. Staphylococcus strains isolated from sonication fuid obtained after removed prothesiscom- ponents ultrasonication and soft tissue bioptates were chatracterised with high ability for bioflm forming. This ability was reliably (p<0.05) higher than in strains isolated from wounds and aspirate. Interspecies diferences were observed in a group of strains isolated from bioptates: optical density of the S. epidermidis stains extracts was reliably (p<0.05) higher than that of S. aureus strains. Conclusion.Bioflm forming ability of Staphylococcus spp. strains isolated from patients with implant-associated infection depends on the biomaterial under study thus defning the most informative methods of microbiological study.

1 Preobrazhenskii PM, Kazemirskii AV, Goncharov MYu. Current views on diagnosing and treatment of patients with periprosthetic infection after the knee arthroplasty. Orthopedic Genius 2016; 3: 94–104
2 Tikhilov RM, Shubnyakov II, Kovalenko AN, et al. Data of hip arthroplasty registry of Vreden Institute for the period 2007–2012 years. Traumatology and Orthopedics of Russia 2013; 3: 167–90
3 Bozhkova SA, Poliakova EM, Borisov AM, Rukina AN. MRSA at prosthesis joint infection: a target for antibacterial therapy. Pharmateca 2015; 4: 65–9
4 Yarets YuI, Shevchenko NI, Rubanov LN. Вynamics of microbal content of chronic wound adjusted for preoperative preparation features. Problems of Health and Ecology 2012; 32 (2): 108–14
5 Oliveira A. Comparison of methods for the detection of bioflm production in coagulase-negative staphylococci. BMC Research Notes 2010; 260 (3): 1–8
6 Ilyina TS, Romanova YuM, Gintsburg AL. Bioflms as a mode of existence of bacteria in external environment and host body: The phenomenon, genetic control, and regulation systems of development. Russian Journal of Genetics 2004; 40 (11): 1189–98
7 Christensen DG, et al. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of Staphylococci to medical devices. Journal of clinical microbiology 1985; 22 (6): 996–1006
8 Afnogenova AG, Darovskaya EN. Microbial bioflms of wounds: status of the issue. Traumatology and Orthopedics in Russia 2011; 3 (61): 119–25
9 Mayansky AN, Chebotar IV. Saphylococcal bioflms: structure, regulation, rejection. Microbiology 2011; 1: 101–8
10 Smirnova TA, et al. Structural and functional characteristics of bacterial bioflms. Microbiology 2010; 79 (4): 413–23
11Bozhkova SA, Krasnova MV, Polyakova EM, et al. Bioflm formation by clinical isolates of S. aureus and S. epidermidis in prosthetic joint infection. Clinical microbiology and antimicrobial chemotherapy 2014; 2 (16): 149–56.

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