Saratov JOURNAL of Medical and Scientific Research

The effect of intraoperative application of Cyclisporin A on the regeneration of the tissues of the anterior segment of the eye in experimentally induced glaucoma

Year: 2018, volume 14 Issue: №4 Pages: 833-837
Heading: Ophtalmology Article type: Original article
Authors: Zhigalskaya Т.А., Krivosheina O.I., Dzyman A.N.
Organization: Siberian State Medical University

Purpose: to study in vivo the features of regeneration of a conjunctiva and sclera after surgery with application of a 0.05% Cyclosporin A against the background of experimentally induced glaucoma. Material and Methods. The experiment was performed in 29 rabbits. At the first stage, the animals were induced with a steroid model of glaucoma by subconjunctival injections of 0.5 ml 0.4% Dexamethasone 1 time per week for 3 months. At the second stage rabbits (24 animals) were subjected to a through incision of the conjunctiva and an incision of the surface layers of the sclera of one of the eyes. Depending on the course of the operation, the animals were divided into the main group and the comparison group. Rabbits of the main group (16 animals) were subjected to local application of a 0.05% Cyclosporin A fora duration of 3 minutes in subgroup "a" (8 animals) and 6 minutes in subgroup "b" (8 animals). In rabbits of the comparison group (8 animals), wasn't used cytostatic. Results. In animals of the comparison group, the inflammatory and reparative reaction, despite the presence of dystrophic changes due to an increase in intraocular pressure, proceeds with the stereotypic dynamics of the change of cellular phases. Using a 0.05% Cyclosporin A suppresses the migration of cells from different populations to the surgical zone, increasing the duration of the macrophage reaction phase and slowing down the transition to the regeneration phase. Conclusion. Using of 0.05% Cyclosporin A during surgical intervention slows down the repair of the tissues of the conjunctiva and sclera, preventing pronounced fibroblation in the area of surgical trauma, which can be an effective way to prevent scarring during antiglaucoma operations.

1 Alekseev VN, Malevannaya OA, Tubadzhi Essam, at al. Progressive glaucoma — inevitability or failure? Glaukoma 2012; (3): 26-32
2 Mamikonyan VR, Petrov SYu, Mazurova YuV, Postoperative use of ranibizumab in increasing the effectiveness sinusstrabeculectomy. Glaukoma 2016; 15 (2): 61-73
3 Salim S. Current variations of glaucoma filtration surgery. Curr Opin Ophthalmol 2012; 23 (2): 89-95
4 Axteryakova IA. Directions of surgical treatment of refractory glaucoma. Ophtalmology: Eastern Europe 2014; 4 (23): 96-104
5 Kladko MA, Takhtaev YuV. Surgery of refractory glaucoma: problems and solutions. Tochka zreniya: East — West 2016; (1): 83-5
6 Fabrikantov OL, Nikolashin SI, Pirogova ES. Surgery of refractory glaucoma: indications, complications, outcomes. Medicina 2016; 21 (1): 204-7
7 Сахнов С. H., Науменко В. В., Волик С. А. и др. Способ хирургического лечения рефрактерной глаукомы. Глаукома 2013; (1): 29-34
8 Petrov SYu, Antonova АА, Makarova AS. Possibilities of prolongation of the hypotensive effect of trabeculectomy. Vestnik oftalmologii 2015; 131 (1): 75-81
9 Al Nemer DM. Results of combined drainage surgery of refractory forms of glaucoma. Medicinskij vestnik Bashkortostana 2014; 9 (2): 28-30
10 Bettin Р, Di Matteo F. Glaucoma: present challenges and future trends. Ophthalmic Res 2013; 50 (4): 197-208

2018_4-2_833-837.pdf278.29 KB

No votes yet