№3, 2017, volume 13

Objective: to study the psycho-emotional state of pregnant women with syphilis. Materials and Methods. The study included 53 pregnant women who had newly registered syphilis. The mean age of patients was 25±2.3 years. Diagnostics was done by Beck's depression inventory, the technique of diagnostics of social frustration level of L. I. Was-serman (modified by V. V. Boiko). Results. Patients had high level of depression on the Beck's method, the technique of diagnostics of social frustration level of L. I. Wasserman (modified by V. V. Boiko) revealed that dissatisfaction with social outcomes in major aspects of life in the majority of patients causes a "position in society" and "life in General" after conducting corrective treatment psycho-emotional state of patients improved. Conclusion. While the use of psychotherapy in combination with drug treatment, syphilis treatment becomes more efficient: decreases the level of depression decreases the level of social frustration in the problematic spheres of life.

The purpose was to study the depth and nature of the zones of thermal damage to the skin under radio wave and laser skin dissection during experiment. Material and Methods. The model of acute thermal damage was full-liner skin wounds of 20 nonlinear rats that were divided into 2 groups and operated by different methods. In the 1st group, the incisions were made by the apparatus of radio wave surgery (Surgitron DF S5), in the 2nd group the animals were operated with a laser surgical apparatus. The magnitude and structure of the lateral thermal damage was evaluated when analyzing the biopsy material. Results. During the study of experimental wounds, the extent of carbonation in the first group (operated with Surgitron DF S5) was 11.56±3.056 urn, coagulation necrosis 116.5±26.78 urn, and the hyper-thermiazone 148.42±60.171 urn. In the group of animals operated with a laser apparatus, the carbonization zone was 22.58±6.62 urn, the coagulation necrosis zone was 331.1±79.08 urn, and the hyperthermia extent was 376.2±53.27 urn. Conclusion. A comparative study of lateral skin damage in radio wave and laser skin dissection revealed a deeper thermal change in the skin and an increase in the extent of thermally altered structures under laser action: the carbonization zone was larger than for radio waves by 11.02 urn, coagulation necrosis by 214.6 urn, and the hyperthermia zone by 227.78 urn.

Objective: to investigate the effect of phototherapy on the level of DNA damage in mononuclear blood cells in patients with psoriasis using DNA Comet Assay. Material and Methods. 87 patients with psoriasis were included into the group treated by photochemotherapy; and 58 patients composed the group receiving 311 nm narrow-band phototherapy. Results. The median mean values for the median of DNA damage in the mononuclear cells under phototherapy exposure do not exceed the standard reference range. While performing a visual study of the dynamics of DNA damage level in the nucleated cells, the patients with the parameters exceeded the initial values and upper level of the reference range have been identified. UV-induced increase of DNA damage level in the mononuclear blood cells may indicate the slowing down the activity of DNA repair system processes. A predictive model for DNA damage final level from the initial values under phototherapy has been obtained, allowing identifying the patients with increased sensitivity to ultraviolet irradiation and slowing down the processes of DNA excision nucleotide repair. Conclusion. Biomonitoring of DNA damage level in the nucleated blood cells under photochemotherapy exposure and UVB-311nm demonstrates the efficacy of nucleotide excision repair processes in the studied groups of patients with psoriasis and the safety of phototherapy in compliance with medical technology.

Objective: to conduct a clinical study of the effectiveness of the gel based on chitosan in skin care healthy people with different manifestations of signs of aging and patients with pigmented lesions of the skin of those aged 34 to 63 years. Material and Methods. The study was performed in the group of patients consisting of 10 people ranging in age from 34 to 63 years. They have been estimated by the following biophysical parameters: the moisture level and elasticity of the skin, the melanin content, the level of erythema, as well as the width and depth of wrinkles. Results. The application of hydrogel with chitosan marked hydration of the skin (in particular immediate increase in skin moisture immediately afterthe first application), reducing the depth and width of wrinkles, restoring elasticity and improving muscle tone. Conclusion. Course application of the hydrogel during the month allows you to obtain a lasting effect, OMO application.

Aim: preliminary studies have made it possible to establish for the polymer a broad spectrum of antimicrobial activity and to attribute it to low-toxic compounds. Material and Methods, the paper presents a comparative evaluation of the treatment of mild and moderate acne with the standard preparation adapalen+clindamycin and cosmetic product Mellisol to change the indices of the dermatological acne index (DAI). Results. The use of the cosmetic product "Mel-lisol" contributed to a significant reduction in the indices of CFU of bacteria. Conclusions. Using of the cosmetic product "Mellisol" is effective in the treatment of acne and showed its high efficacy and safety in the treatment of patients with mild and moderate acne.

The violation of the angiogenesis is associated with pathogenesis of many diseases, but especially pronounced pathological angiogenesis underlies the growth of tumors and metastasis. The physiological control of the angiogenesis is carried out by many growth factors, one main factor — the vascular endothelial growth factor (VEGF), realizing their effects by receptors — proteinkinases. The target therapy of various neoplastic diseases, based on the suppression of angiogenesis, aimed at blockade of receptors for VEGF and other pro-angiogenic growth factors. However, the medicine blockade of receptors for growth factors and suppression of tumor angiogenesis leads to inappropriate exposure are the main cells of the dermis — fibroblasts. As a result of changing the regulatory mechanisms of inhibition of the physiological renewal of epidermis, suppression of angiogenesis and repair when damaged skin there are various skin toxic reactions that are dependent on receptor targets of angiogenesis inhibitors. The study of the mechanisms of adverse events of targeted therapy is an important way of oncoimmunology and dermatology, which will further help to determine the optimal scheme of correction of dermal toxicity and maximize the effectiveness of the antitumor therapy.

Aim: to determine the effectiveness of the photosensitizer "Curcumin" in various concentrations (0.15%, 1%, 1.5%) in combination with visible spectrum radiation in the treatment of nail psoriasis. Material and Methods. 6 patients (4 women and 2 men) of different age groups (from 20 to 50 years) with the established diagnosis of "Psoriasis" were observed. The study was carried out using the "Curcumin" photosensitizer solution, the broadband spectrometer "Ocean Optics USB4F01891", the LS-55 spectrometer, the PhotoDyn-750 and the THORLABS PM100D. To assess the dynamics of the pathological process, the N-NAIL Index was used. Results. The most pronounced and clinically significant changes were detected when using a 1.5% solution of the "Curcumin" photosensitizer. Conclusion. The photosensitive agent "Curcumin" is effective in photodynamic therapy of psoriasis of the nails.

 Objective: to evaluate the structure and volume of expenditures on treatment of patients with different clinical forms of psoriasis using the method of analysis of "cost of illness". Material and Methods. The study was conducted on the basis of the Clinic for Skin and Venereal Diseases Medical University of the Saratov State Medical University n.a. V. I. Razumovsky. To determine the costs of treating patients with different clinical forms of psoriasis, 702 medical records of a stationary patient have been analyzed. To assess and analyze the costs of treating patients with psoriasis, one of the methods of clinical and economic analysis was used-the "cost of illness". In the analysis of costs, the costs of inpatient treatment were included, which included direct medical costs, including the payment of medical personnel, the costs of diagnostic, medical manipulation, medicines, the use of medical equipment, inventory, etc.; indirect costs, including the costs of ensuring the activities of the medical institution. The comparative characteristic of the evaluation of the cost of treatment of patients with different forms of psoriasis in accordance with the program of state guarantees and the actual costs of the clinic. Results. It was revealed that the actual cost of the treatment for the completed case of patients with severe forms of psoriasis is 3 times higher than the cost of the treatment under the state guarantees program. The study of the structure of expenditures showed that the main costs are for the purchase of medicines and supplies in severe forms of psoriasis. Conclusion. The difference between the cost of treatment for patients with different forms of psoriasis according to the territorial program of state guarantees of the Saratov region and the actual costs is explained, because the state guarantees program does not take into account comorbid pathology and concomitant diseases, patient's age, which requires additional diagnostic and treatment methods. Analysis of the structure of actual expenditures showed that the maximum share of costs is accounted for by medicines and supplies in severe forms of psoriasis.

Objective: to study the factors of increased risk of hyperpigmentation, with the polymorphism of MMP1, XRCC1, HFE (2), GSTT genes and analysis of their association in patients with various clinical forms of non-neoplastic melanin hyperpigmentation. Material and methods. The study included 38 patients with hyperpigmentation (melasma / chlo-asma, post-traumatic/ post-inflammatory hyperpigmentation, solar lentigo). All patients were underwent buccal swabs with sterile cotton buds to take the material for the study. All patients were underwent genetic analysis of polymorphism of HFE (2), XRCC1, MMP1, and GSTT genes by genotyping single-nucleotide substitutions. Results. In patients with melasma / chloasma, the high value of the alleles cipher of MMP1, XRCC1, and GSTT genes was noted, while the cipher of HFE (rs1799945) and HFE (rs1800562) allele index showed average values. In patients with post-traumatic / post-inflammatory pigmentation, the high value ofthe alleles cipher of MMP1, HFE (rs1799945) and HFE (rs1800562), GSTT genes was noted, while XRCC1 allele cipher showed average values. In patients with solar lentigo, the high value ofthe alleles cipher of MMP1, XRCC1, and GSTT genes was noted, while the cipher of HFE (rs1799945) and HFE (rs1800562) allele index showed average values. Conclusion. Was revealed the similarity ofthe results of genetic research in patients with melasma / chloasma and in patients with solar lentigo.

The purpose of the research was to study the diagnostic possibilities of the use of the active radiometry method for localized scleroderma (LS), both for monitoring the results of therapy, and for a comprehensive assessment of the functional state of body tissues in the study area, as well as the possibility of determining new foci of the disease. Material and Methods. In a pilot study, patients were presented with different forms of lesions and their different localization, as well as with different disease activity. The method of resonance wave diagnostics is implemented in the diagnostic complex "Aquafon". Results. The primary registration of wave activity in different types of foci may differ. The activity of the foci and the possibility of the appearance of new foci can be said not so much by the data of the initial measurements as by the integral data on their difference before and after the illumination procedure, which requires further study. Conclusion. It is necessary to improve the measurement technique, avoid places where the sensor is not well attached to the skin or minimize this effect. The research also aimed to conduct studies with simultaneous use of other methods.