Saratov JOURNAL of Medical and Scientific Research

Dynamics of laser speckle imaging of blood flow in the tissues at partial temporary pancreatic ischemia of hungry, fed and alcoholized rats

Year: 2016, volume 12 Issue: №2 Pages: 106-109
Heading: Macro- and Micromorphology Article type: Original article
Authors: Alexandrov D.A., Timoshina P.A., Tuchin V.V., Maslyakova G.N., Palatova T.V., Sedov D.S., Izmailov R.R.
Organization: Saratov State Medical University

The aim of the study is to determine the influence of a partial temporary ischemia and reperfusion on the development of pancreatitis in laboratory rats. Material and Methods. The experiments were performed on 73 white rats. Occluded blood vessels and blood flow were recorded by digital biomicroscopy and laser speckle contrast imaging. Statistical data processing was carried out the program Statistica for Windows 6.0 using the Student's criterion. Results. Laser speckle imaging and digital microscopes provide the monitoring of blood flow in the vessels of the pancreas. After a five-minute partial ischemia reactive hyperemia occurs, changes are invertible. After a twenty-minute partial ischemic reactive hyperemia does not occur, without developing pancreatitis necrosis. Pancreas of alcoholized rats has been found out to be more sensitive to ischemia. Conclusion. The method of laser speckle imaging and intravital microscopy after digital five- and twenty-minute partial ischemia of the pancreas revealed phase opposite directed changes in its tissue perfusion and varying severity of postischemic pancreatitis.

1. Beger HG, Isenmann R. Surgical manangement of necrotizing pancreatitis. Surg Clin N Amer 1999; 79 (4): 793-800
2. Todd H, Baron MD, Desiree E, Morgan MDN. Acute Necrotizing Pancreatitis. Engl J Med 1999; 341 (6): 460
3. Menger MD, Bonkhoff H, Vollmar B. Ischemia-reperfusion-induced pancreatic microvascular injury: an intravital fluorescence microscopic study. Dig Dig Sci 1996; 41: 823-830
4. Briers JD. Laser Doppler, speckle and related techniques for blood perfusion mapping and imaging. Physiological Measurement 2001; 22: 35-66
5. Goodman JW. Speckle Phenomena in Optical: Theory and Applications. Englewood, Colorado: Roberts and Company, 2007; 387 p.
6. Hoffmann TF, Kubler J, Mebmer K. Bradykinin — Antagonisiering bei Ischamie und Reperfusion des Pankreas. ZentralblChir1996; 121:412-422
7. Luo Q, Jiang C, Li P, et al. Laser speckle imaging of cerebral blood flow: Chapter 5. In: Tuchin W, ed. Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring and Material Science. 2nd ed. Vol. 1. Springer-Verlag, Berlin, Heidelberg, N. Y, 2013; 167-211 p.
8. Saveliev V.S., Buyanov V.M., Ognev J.V. Acute pancreatitis. M: Medicine, 1983; 240 p.
9. Uhl W, WarshawA, Clement. Imrie and al IAP Guidelines for the Surgical: Management of Acute Pancreatitis. Pancreatology 2002; (2): 565-573
10. Alexandrov D.A., Timoshina P.A., Tuchin V.V., Maslyakova G.N., et al. Dynamics of laser speckle imaging of blood flow and morphological changes in tissues with a full time local ischemia of pancreas. Saratov Journal of Medical Scientific Research 2014; 10 (4): 596-600.

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