Saratov JOURNAL of Medical and Scientific Research

Immunogenetic markers of Crohn's disease in adults population of the Moscow region

Year: 2014, volume 10 Issue: №4 Pages: 743-749
Heading: Genetics Article type: Original article
Authors: Stavtsev D.S., Astrelina Т.А., Azova M.M., Lebedeva L.L., Pukhlikova T.V., Chumak A.A., Knyazev O.V., Eremin I.I., Gilmutdinova I.R., Kotenko K.V.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, Peoples' Friendship University of Russia, Blood transfusion station of the Department of Health of the City of Moscow

Aim: to study immunogenetic markers of predisposition to the development and protection for Crohn's disease in adults population of the Moscow region. Material and methods. The study included 53 samples of peripheral blood of patients with Crohn's disease in the Moscow region. The control group was represented by 1,700 samples of umbilical cord blood is healthy newborns. Revealing HLA antigens at low level performed by SSO method on DynalRELI 48 processor. The results received with ambiguous interpretation was using PCR-SSP method (Ivitrogen). Results. Were found the positive and negative associations of groups of HLA alleles with clinical form, the course of Crohn's disease and response to steroid treatment, in particular revealed that, predisposition to the development for Crohn's disease in women and with sensitivity to steroid treatment in this disease associated allele group C*12, to the characteristic restricting markers such as Crohn's disease include the В 38 and A*11 markers nonrestricting, nonpenetrating noninflammatory type groups are alleles B*56 and C*14 and C*14 is also associated with the risk of Crohn's disease in men, characteristic markers of protection to the development of the disease crown with chronic relapsing and severe clinical course are DQB1*02 and DQB1*03, respectively. Conclusion. These results demonstrate the need for studies of gene polymorphism HLA-system, not only in relation to the disease in general, but in selected patients with clinical groups.

1. Belousova EA Resistant forms of inflammatory bowel disease. DSc: abstract. Moscow, 1998; 38 p.
2. Satsangi J, Morecroft NB, Shah, et al. Genetics of inflammatory bowel disease: scientific and clinical implications. Best Pract Res Clin Gastroenterol 2003; 17 (1): 3-18
3. Molodecky NA, Soon IS, Rabi DM. Increasing incidence and prevalence of the inflammatory bowel diseases with time, based on systematic review. Gastroenterology 2012; 142 (1): 46-54
4. Ponder AA, Long MD. Clinical review of recent findings in the epidemiology of inflammatory bowel disease. Clinical Epidemiology 2013; 5: 237-247
5. Nikulina IV. Clinical and epidemiological characteristics of inflammatory bowel disease in the Moscow region: PhD abstract. Moscow, 1997; 24 p.
6. Belousova EA. Ulcerative colitis and Crohn's disease. Tver: LLC «Publisher» triad», 2002; 128 p.
7. Nikolaev NN, Chechetkina ID, Nikolaev LV. Epidemiology of ulcerative colitis and Crohn's disease in the Krasnoyarsk Territory. Russian Journal of Gastroenterology, Hepatology Coloproctology 2004; 5 (XIV, 23): 1.
8.TkachevAV, DevlikamovaTA, RosenbergTG.Assessingthe prevalence of inflammatory bowel disease (IBD) in the Rostov region. Gastroenterology. Symposium in Southern Russia, Rostov-on-Don, 2009; p. 103
9. Trachtenberg EA, Yang Н, Hayes Е, et al. HLAclass II hap-lotype associations with inflammatory bowel disease in Jewish (Ashkenazi) and non-Jewish Caucasian populations. Hum Immunol 2000; 61 (3): 326-33
10. Silverberg MS, Mirea L, Bull SB. A Population- and Family-Based Study of Canadian Families Reveals Association of HLA DRB1
0103 With Colonic Involvement in Inflammatory Bowel Disease. Inflammatory Bowel Diseases 2003; 9 (1): 1-9
11. Fernandez L, Mendoza JL, Martinez A. IBD1 and IBD3 Determine Location of Crohn's Disease in the Spanish Population. Inflammatory Bowel Diseases 2004; 10 (6): 715-722
12. Lappalainen M, Halme L, Turunen U, et al. Association of IL23R, TNFRSF1A, and HLA-DRB1
0103 allele variants with inflammatory bowel disease phenotypes in the Finnish population. Inflamm Bowel Dis 2008; 14(8): 1118-24
13. GulwaniDAkolkar B, Akolkar PN, Lin XY, et al. HLA Class II Alleles Associated with Susceptibility and Resistance to Crohn's Disease in the Jewish Population. Inflammatory Bowel Diseases 2000; 6 (2): 71-6
14. Adler G. Crohn's disease and ulcerative colitis. M.: GEOTAR-Med, 2001; 500 p.
15. Okada Y, Yamazaki К, Umeno J. HLA-Cw
1502 haplotype increases risk for ulcerative colitis but reduces risk for Crohn's disease. Gastroenterology 2011; 141 (3): 864-71
16. Kawasaki A, Tsuchiya N, Hagiwara K, et al. Independent contribution of HLA-DRB1 and TNF alpha promoter polymorphisms to the susceptibility to Crohn's disease. Genes Im-mun2000; 1 (6): 351-7
17. Annese V, Lombardi G, Perri F. Variants of CARD15 are associated with an aggressive clinical course of Crohn's disease: an IG-IBD study. American Journal of Gastroenterology 2005; 100:84-92
18. Hoentjen F, Tonkonogy L, Dieleman L, et al. CD4 T lymphocytes mediate colitis induced by non-pathogenic Bacte-roides vulgatus in HLA-27 transgenic rats. Gastroenterol 2005; 128(4):A-206
19. Morozova NA. Clinical and genetic relationship with inflammatory diseases of the colon (ulcerative colitis and Crohn's disease): PhD abstract. Moscow, 1997; 24 p.
20. Khalif IL, Loranskaya ID. Inflammatory bowel disease (ulcerative colitis and Crohn's disease): clinical features, diagnosis and treatment. M.: Miklosh, 2004; 88 p.
21. Russian Gastroenterological Association recommendations for the treatment of Crohn's disease in adults (draft). Russian Journal of Gastroenterology, Hepatology, Col-oproctology 2012; 23 (6): 68-82
22. Silverberg MS, Satsangi J, Ahmad T, et al. Toward an integrated clinical, molecular and serological classification of inflammatory bowel disease: report of a working party of the 2005 Montreal World Congress of Gastroenterology. Can J Gastroen-terol 2005; 19 (Suppl. A); 5-36
23. Sokolova El. Clinical Immunology. M.: Medicine, 1998; 45 p.
24. Haitov RM. Immunology. М.: GEOTAR-Med, 2009; 320 p.
25. Gough SCL, Simmonds MJ. The HLA Region and Autoimmune Disease: Associations and Mechanisms of Action. Current Genomics 2007; 8: 453-465
26. Bugawan TL, Mack SJ, Stoneking M, et al. HLA class I distributions in six Pacific/Asian populations: evidence of selection at the HLA-A locus. Tissue Antigens 1999; 53: 311-319
27. Begovich AB, Moonsamy PV, Mack SJ, et al. Genetic variability and linkage disequilibrium within the HLA-DP region: analysis of 15 different populations. Tissue Antigens 2001; 57: 424-439
28. Sanchez-Mazas A, Meyer D. The Relevance of HLA Sequencing in Population Genetics Studies. Journal of Immunology Research 2014; article ID 971818. http://dx.doi.Org/10.1155/2014/971818
29. Boldyreva MN. HLA (class II) and natural selection: "Functional" genotype hypothesis advantages of "functional" heterozygosity: DSc abstract. Moscow, 2007; 47 p.
30. Khromova NA Polymorphism of HLA system in representatives of different ethnic groups of Slavic (Russian, Belaru-sian and Ukrainian): PhD abstract. Moscow, 2006; 29 p.
31. Loranskaya ID, Khalif IL, Dolbin AG, Yazdovs-kiy VV. Genetic markers HLA-nonspecific inflammatory and functional diseases of the colon. Russian medical news 2001; 2: 43-46.

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