Saratov JOURNAL of Medical and Scientific Research

Dynamics of laser speckle imaging of blood flow and morphological changes in tissues with a full time local ischemia of pancreas

Year: 2014, volume 10 Issue: №4 Pages: 596-600
Heading: Macro- and Micromorphology Article type: Original article
Authors: Alexandrov D.A., Timoshina P.A., Tuchin V.V., Maslyakova G.N., Palatova T.V., Skorokhod A.A., Lukashevich K.A., Yarovoy A.S..
Organization: Saratov State Medical University, Saratov state university

The purpose: to establish influence of a full ischemia of different duration and the subsequent reperfusionon pathology development in pancreas of rats by means of laser speckle-visualization and lifetime digital microscopy. Materials and Methods. The work has been performed on 42 white rats of line Wistar in weight of 200-250 Research of properties of a blood-groove was made by means of methods laser Doppler flowmetry, digital biomicroscopy and a method of laser speckle-contrast visualization. Results. After the termination of a 5-minute full ischemia the speed of bloodflow has been increased in 2-3 times, clinic pancreatic necrosis is marked does not develop. After the termination of 20-minute full ischemia the increase in speed of a bloodflow did not occur, there were morphological and clinical signs of pancreatic necrosis. Conclusion, the efficiency of monitoring of microhemodynamics of pancreas in rats by the method of speckle-capillary of full field has been shown. Multidirectional phase of perfusion changes in pancreas have been revealed after reversible infringement of blood supply of different duration.

1. Vashetko R.V., Tolstoy A.D., Kurygin A.A., et al. Acute pancreatitis and injury of the pancreas. SPb.: Piter, 2000; 320 p.
2. Briers J.D. Laser Doppler, speckle and related techniques for blood perfusion mapping and imaging. Physiological Measurement 2001; 22: 35-66
3. Goodman J.W. Speckle Phenomena in Optical: Theory and Applications Englewood. Colorado: Roberts and Company, 2007
4. Hoffmann T.F., Kubler J., Mebmer K. Bradykinin-Antagonis-iering bei Ischamie und Reperfusion des PankreasZentralbl. Chir 1996; 121:412-422
5. Saveliev V.S., Buyanov V.M., Ognev J.V. Acute pancreatitis. M: Medicine, 1983. 240 p.
6. Broe P.J., Zuidema G.D., Cameron J.L. The role of ischemia in acute pancreatitis: Studies with an isolated perfused canine pancreas Surgery 1982; 91: 377-382
7. Douzdjian V., Abecassis M.M., Cooper J.L., et al. Incidence, management and significance of surgical complication after pancreatic transplantation. Surg Gynecol Obstet 1993; 177: 451-456
8. Dunn A.K., Bolay H., Moskowitz M.A., Boas D.A. Impact of velocity distribution assumption on simplified laser speckle imaging equation. J Cereb Blood Flow Metab 2001; 21: 195-201
9. Hoffmann T.F., Leiderer R., Waldner H., et al. Ischemia reper-fusion of the pancreas: a new in vivo model for acute pancreatitis in rats. Res Exp Med 1995; 195: 125-144
10. Fernandes-Cruz L., Sabater L., Gialbert R., et al. Native and graft pancreatitis following combined pancreas-renal transplantation. Br J Surg 1993; 80: 1429-1432
11. Vilensky M.A., Agafonov D.N., Zimnyakov D.A., et al. Speckle-correlation analysis of microcirculation of nail bed. Quantum electronics 2011; 41 (4): 324-328
12. Luo Q., Jiang С., Li Р., Cheng Н., Wang Z., Wang Z., Tuchin V.V. Laser speckleimaging of cerebral blood flow. Chapter 5. In: Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring and Material Science, V.1. Ed. by Tuchin V.V., 2ndedition. Springer-Verlag, Berlin, Heidelberg, N.Y., 2013;p. 167-211
13. Busing M., Hopt U.T., Quacken M., et al. Morphological studies of graft pancreatitis following pancreas transplantation. Br J Surg 1993; 80: 1170-1173
14. Menger M.D., Bonkhoff H., Vollmar B. Ischemia-reperfu-sion-induced pancreatic microvascular injury: An intravital fluorescence microscopic study. Dig Dig Sci 1996; 41: 823-830
15. Slater D.N., Bardsley D., Magnall Y., et al. Pancreatic ischemia: sensitivity and reversibility of the changes. Br J Exp Patol 1975;56:530-536.

2014_04_596-600.pdf572.11 KB

No votes yet